Chlamydia psittaci is an obligately intracellular Gram-negative bacterium causing respiratory disease (chlamydiosis) or asymptomatic carriage in birds. C. psittaci is a zoonotic agent causing psittacosis or parrot fever in humans. Vertical and/or horizontal transmission via eggs might have serious repercussions on the C. psittaci infection status of poultry flocks and thus on zoonotic risk for all workers along the poultry supply chain. We therefore studied the presence of C. psittaci in a hatchery. In addition, we examined all (n = 4) employees of the hatchery to evaluate the zoonotic risk. We could not detect C. psittaci on either eggshells or eggshell membranes. However, C. psittaci isolates of different outer-membrane protein A (ompA) genotypes were cultured from the air of both turkey (genotypes A and C) and chicken (genotype D) hatching chambers. Zoonotic transmission occurred in all employees and a mixed infection with up to three different genotypes (A, D and C), also found in air samples, was discovered. Diagnostic monitoring and reporting of C. psittaci infections in poultry workers should be promoted. Additionally, an efficient veterinary vaccine and information campaigns on zoonotic risk and preventive measures against C. psittaci transmission would be beneficial to public health.
ChahotaR.,
KatochR. C.,
SinghS. P.,
VermaS.,
MahajanA.2000; Concurrent outbreak of chlamydiosis and aflatoxicosis among chickens in Himachal Pradesh, India. Veterinarski Arhiv 70:207–213
CoxN. A.,
BerrangM. E.,
CasonJ. A.2000; Salmonella penetration of egg shells and proliferation in broiler hatching eggs – a review. Poult Sci 79:1571–1574[PubMed][CrossRef]
GaedeW.,
RecklingK. F.,
DresenkampB.,
KenkliesS.,
SchubertE.,
NoackU.,
IrmscherH. M.,
LudwigC.,
HotzelH.,
SachseK.2008; Chlamydophila psittaci infections in humans during an outbreak of psittacosis from poultry in Germany. Zoonoses Public Health 55:184–188 [View Article][PubMed]
GeensT.,
DewitteA.,
BoonN.,
VanrompayD.2005; Development of a Chlamydophila psittaci species-specific and genotype-specific real-time PCR. Vet Res 36:787–797 [View Article][PubMed]
HarkinezhadT.,
GeensT.,
VanrompayD.2009; Chlamydophila psittaci infections in birds: a review with emphasis on zoonotic consequences. Vet Microbiol 135:68–77 [View Article][PubMed]
HSE (2010). Infection at work: controlling the risks. A guide for employers and the self employed on identifying, assessing and controlling the risks of infection in the workplace. Advisory Committee on Dangerous Pathogens
LaroucauK.,
de BarbeyracB.,
VorimoreF.,
ClercM.,
BertinC.,
HarkinezhadT.,
VerminnenK.,
ObenicheF.,
CapekI. et al.2009a; Chlamydial infections in duck farms associated with human cases of psittacosis in France. Vet Microbiol 135:82–89[PubMed][CrossRef]
LaroucauK.,
VorimoreF.,
AazizR.,
BerndtA.,
SchubertE.,
SachseK.2009b; Isolation of a new chlamydial agent from infected domestic poultry coincided with cases of atypical pneumonia among slaughterhouse workers in France. Infect Genet Evol 9:1240–1247 [View Article][PubMed]
StingR.,
LerkeE.,
HotzelH.,
JodasS.,
PoppC.,
HafezH. M.2006; [Comparative studies on detection of Chlamydophila psittaci and Chlamydophila abortus in meat turkey flocks using cell culture, ELISA, and PCR]. Dtsch Tierarztl Wochenschr 113:50–54 (in German) [PubMed]
Van DroogenbroeckC. M.,
Van RisseghemM.,
BraeckmanL.,
VanrompayD.2009a; Evaluation of bioaerosol sampling techniques for the detection of Chlamydophila psittaci in contaminated air. Vet Microbiol 135:31–37[PubMed][CrossRef]
Van DroogenbroeckC.,
BeeckmanD. S.,
VerminnenK.,
MarienM.,
NauwynckH.,
BoesingheL. T.,
VanrompayD.2009b; Simultaneous zoonotic transmission of Chlamydophila psittaci genotypes D, F and E/B to a veterinary scientist. Vet Microbiol 135:78–81 [View Article][PubMed]
Van LoockM.,
GeensT.,
De SmitL.,
NauwynckH.,
Van EmpelP.,
NaylorC.,
HafezH. M.,
GoddeerisB. M.,
VanrompayD.2005a; Key role of Chlamydophila psittaci on Belgian turkey farms in association with other respiratory pathogens. Vet Microbiol 107:91–101 [View Article][PubMed]
Van LoockM.,
VerminnenK.,
MessmerT. O.,
VolckaertG.,
GoddeerisB. M.,
VanrompayD.2005b; Use of a nested PCR-enzyme immunoassay with an internal control to detect Chlamydophila psittaci in turkeys. BMC Infect Dis 5:76 [View Article][PubMed]
VanrompayD.,
DucatelleR.,
HaesebrouckF.1992; Diagnosis of avian chlamydiosis: specificity of the modified Giménez staining on smears and comparison of the sensitivity of isolation in eggs and three different cell cultures. Zentralbl Veterinarmed B 39:105–112[PubMed][CrossRef]
VanrompayD.,
Van NeromA.,
DucatelleR.,
HaesebrouckF.1994; Evaluation of five immunoassays for detection of Chlamydia psittaci in cloacal and conjunctival specimens from turkeys. J Clin Microbiol 32:1470–1474[PubMed]
VanrompayD.,
DucatelleR.,
HaesebrouckF.1995a; Chlamydia psittaci infections: a review with emphasis on avian chlamydiosis. Vet Microbiol 45:93–119 [View Article][PubMed]
VanrompayD.,
MastJ.,
DucatelleR.,
HaesebrouckF.,
GoddeerisB.1995b; Chlamydia psittaci in turkeys: pathogenesis of infections in avian serovars A, B and D. Vet Microbiol 47:245–256 [View Article][PubMed]
VerminnenK.,
Van LoockM.,
HafezH. M.,
DucatelleR.,
HaesebrouckF.,
VanrompayD.2006; Evaluation of a recombinant enzyme-linked immunosorbent assay for detecting Chlamydophila psittaci antibodies in turkey sera. Vet Res 37:623–632 [View Article][PubMed]
WittenbrinkM. M.,
MrozekM.,
BispingW.1993; Isolation of Chlamydia psittaci from a chicken egg: evidence of egg transmission. Zentralbl Veterinarmed B 40:451–452[PubMed]