1887

Abstract

This study focused on identifying possible new options derived from natural sources for the treatment of bacterial infections. Several natural products were investigated for their potential in modulating –host-cell interactions. The proliferation of was effectively inhibited inside HEp-2 cells in the presence of 4-methoxycinnamic acid and propolin D. Propolin D also significantly reduced the apoptosis of infected macrophage-like U937 cells and moderately reduced the secretion of interleukin (IL)-1β and IL-18, which probably resulted from the inhibition of invasion plasmid antigen B secretion by this compound. Further characterization showed that propolin D did not prevent escape of from phagocytic vacuoles, as evidenced by actin-based motility and by the fact that addition of chloroquine did not further reduce the number of intracellular c.f.u. The role of propolin D in modulating autophagy could not be established under the experimental conditions used. As these compounds had no direct anti- activity , it was concluded that these compounds modulated –host-cell interactions by targeting yet-to-be defined mechanisms that provide benefits to host cells.

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2011-11-01
2019-10-23
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vol. , part 11, pp. 1626 - 1632

Influence of chloroquine and an autophagy inducer and inhibitor on proliferation inside HEp-2 cells

Detection of IpaB secretion

. Confocal fluorescent microscopy of intracellular [Single PDF file](142 KB)



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