1887

Abstract

Novel real-time PCR assays targeting the insertion sequence IS, the toxin promoter region and insertion sequence IS were designed. PCR assays were capable of detecting ≤10 copies of target DNA per reaction, with an amplification efficiency of ≥90 %. From September 2003 to December 2009, per-nasal swabs and nasopharyngeal aspirates submitted for culture from patients ≤1 month to >15 years of age were examined by real-time PCR. Among 1324 patients, 76 (5.7 %) were culture positive and 145 (10.95 %) were PCR positive. Of the PCR-positive patients, 117 (81 %) were aged 6 months or less. A total of 1548 samples were examined, of which 87 (5.6 %) were culture positive for and 169 (10.92 %) were PCR positive. All culture-positive samples were PCR positive. Seven specimens (0.5 %) were culture positive and 10 (0.8 %) were PCR positive, with all culture-positive samples yielding PCR-positive results. A review of patient laboratory records showed that of the 1324 patients tested for pertussis 555 (42 %) had samples referred for respiratory syncytial virus (RSV) testing and 165 (30 %) were positive, as compared to 19.4 % of the total 5719 patients tested for RSV in this period. Analysis of the age distribution of RSV-positive patients identified that 129 (78 %) were aged 6 months or less, similar to the incidence observed for pertussis in that patient age group. In conclusion, the introduction of the real-time PCR assays for the routine detection of resulted in a 91 % increase in the detection of the organism as compared to microbiological culture. The incidence of infection with is low while the incidence of RSV infection in infants suspected of having pertussis is high, with a similar age distribution to infection.

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2011-06-01
2020-01-28
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