1887

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Volume 60, Issue 7

Evaluation of PCR-based screening for vancomycin-resistant enterococci compared with a chromogenic agar-based culture method Free

Abstract

Rapid detection of vancomycin-resistant enterococci (VRE) infection is very important for control and prevention of nosocomial spread of these bacteria. A multiplex PCR method for rapid screening of VRE has recently been developed. We performed a prospective study of VRE screening tests to compare the performance of PCR to that of a chromogenic agar-based culture method. From January to December 2009, a total of 8815 rectal swab specimens were tested simultaneously for VRE by VRE selective culture and by PCR. The specimens were inoculated onto ChromID VRE agar containing 8 µg vancomycin ml and examined after 24 and 48 h of incubation. Identification and antibiotic susceptibility tests were performed using the automated VITEK-2 system and a supplementary E-test and disk diffusion test. Detection of the and genes was performed with the Seeplex VRE detection kit. Specimens were inoculated in enterococcosel broth for 16–24 h before PCR for enrichment of VRE. VRE were isolated from 741 of the 8815 specimens by chromogenic agar-based culture (8.4 %). and genotypes were detected in 758 (8.6 %) and 3 (0.03 %) specimens, respectively, by multiplex PCR. Sensitivity, specificity, positive predictive value and negative predictive value of PCR for detection of VRE were 98.2 %, 99.6 %, 95.7 %, and 99.8 %. No VRE were isolated from -positive specimens. The overall performance of PCR is comparable to that of a chromogenic agar-based culture method for screening of VRE, so PCR could be an alternative or supportive method for effective control of nosocomial VRE infection.

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© 2011 SGM
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2011-07-01
2024-04-19
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Evaluation of PCR-based screening for vancomycin-resistant enterococci compared with a chromogenic agar-based culture method
J. Med. Microbiol. 60, 945 (2011); https://doi.org/10.1099/jmm.0.029777-0
/content/journal/jmm/10.1099/jmm.0.029777-0
/content/journal/jmm/10.1099/jmm.0.029777-0
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