1887

Abstract

This study was designed to investigate the molecular epidemiology and antibiotic-resistance characteristics of 11 carbapenem-resistant clinical isolates of obtained in Norway between 2004 and 2009. Interestingly, all the isolates were linked with recent hospitalization outside Norway. The epidemiological status was investigated by multilocus sequence typing (MLST), multiplex PCR assays for major international clones, typing of -like variants and PFGE. The genotypic-resistance characteristics, including the occurrence of OXA-carbapenemase-encoding and 16S rRNA methylase-encoding genes and class 1 integrons, were investigated by PCR assays and sequencing. Seven isolates were found to harbour and belong to MLST clonal complexes (CCs) CC2 (Pasteur Institute scheme) and CC92 (Bartual scheme), and international clone II. One isolate harboured , and belonged to CC1, CC109 and international clone I. Two isolates belonged to sequence group 9, probably a subgroup of international clone I, and one isolate belonged to sequence group 4, a proposed novel international clone. All isolates contained an acquired OXA-carbapenemase-encoding gene: -like (=9), -like (=1) and -like (=1). Four isolates with high-level aminoglycoside-resistance contained the 16S rRNA methylase-encoding gene. Class 1 integrons with six different variable regions were detected. Sequence analysis of gene cassettes identified four aminoglycoside (, , and ), two chloramphenicol ( and ), one -lactamase ( ) and one rifampicin () resistance gene in various combinations. In conclusion, the occurrence of isolates producing OXA carbapenemase and 16S rRNA methylase in Norway was related to the worldwide distribution of international clones I and II, and the emergence of novel international clones.

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2011-04-01
2019-12-08
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vol. , part 4, pp. 515 - 521

[ PDF file] (144 KB) Primers used for molecular characterization of the isolates.



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