1887

Abstract

In the present work we report for what we believe to be the first time the antifungal activity and mechanism of action of the essential oils of from Portugal. The essential oils were isolated by hydrodistillation and analysed by GC and GC/MS. The MIC and the minimal lethal concentration (MLC) of the essential oil and its major compounds were determined against several pathogenic fungi. The influence of subinhibitory concentrations of the essential oil on the dimorphic transition in was also studied, as well as propidium iodide and FUN-1 staining of cells by flow cytometry following short treatments with the essential oil. The oils were characterized by a high content of oxygen-containing monoterpenes, with 1,8-cineole being the main constituent. Monoterpene hydrocarbons were present at lower concentrations. According to the determined MIC and MLC values, the dermatophytes and were the most sensitive fungi (MIC and MLC values ranging from 0.32 to 0.64 µl ml), followed by species (at 0.64–2.5 µl ml). For most of these strains, MICs were equivalent to MLCs, indicating a fungicidal effect of the essential oil. The oil was further shown to completely inhibit filamentation in at concentrations well below the respective MICs (as low as MIC/16). Flow cytometry results suggested a mechanism of action ultimately leading to cytoplasmic membrane disruption and cell death. Our results show that essential oils may be useful in the clinical treatment of fungal diseases, particularly dermatophytosis and candidosis, although clinical trials are required to evaluate the practical relevance of our research.

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2011-05-01
2019-11-20
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References

  1. Adams R. P. . ( 1995; ). Identification of Essential Oil Components by Gas Chromatography/Mass Spectroscopy. Carol Stream, IL:: Allured Publishing Corporation;.
    [Google Scholar]
  2. Angioni A. , Barra A. , Coroneo V. , Dessi S. , Cabras P. . ( 2006; ). Chemical composition, seasonal variability, and antifungal activity of Lavandula stoechas L. ssp. stoechas essential oils from stem/leaves and flowers. . J Agric Food Chem 54:, 4364–4370. [CrossRef] [PubMed]
    [Google Scholar]
  3. Bakkali F. , Averbeck S. , Averbeck D. , Idaomar M. . ( 2008; ). Biological effects of essential oils – a review. . Food Chem Toxicol 46:, 446–475. [CrossRef] [PubMed]
    [Google Scholar]
  4. Cavaleiro C. , Salgueiro L. R. , Miguel M. G. , Proença da Cunha A. . ( 2004; ). Analysis by gas chromatography-mass spectrometry of the volatile components of Teucrium lusitanicum and Teucrium algarbiensis . . J Chromatogr A 1033:, 187–190. [CrossRef] [PubMed]
    [Google Scholar]
  5. Cavaleiro C. , Pinto E. , Gonçalves M. J. , Salgueiro L. R. . ( 2006; ). Antifungal activity of Juniperus essential oils against dermatophyte, Aspergillus and Candida strains. . J Appl Microbiol 100:, 1333–1338. [CrossRef] [PubMed]
    [Google Scholar]
  6. Cavanagh H. M. A. , Wilkinson J. M. . ( 2002; ). Biological activities of lavender essential oil. . Phytother Res 16:, 301–308. [CrossRef] [PubMed]
    [Google Scholar]
  7. CLSI (2008a). Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standard, 3rd edn. M27-A3. Wayne, PA: Clinical and Laboratory Standards Institute.
  8. CLSI (2008b). Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi; Approved Standard, 3rd edn. M38-A2. Wayne, PA: Clinical and Laboratory Standards Institute.
  9. Council of Europe ( 1997; ). European Pharmacopoeia, , 3rd edn.. Strasbourg:: Council of Europe;.
    [Google Scholar]
  10. Dadalioğlu I. , Evrendilek G. A. . ( 2004; ). Chemical compositions and antibacterial effects of essential oils of Turkish oregano (Origanum minutiflorum), bay laurel (Laurus nobilis), Spanish lavender (Lavandula stoechas L.), and fennel (Foeniculum vulgare) on common foodborne pathogens. . J Agric Food Chem 52:, 8255–8260. [CrossRef] [PubMed]
    [Google Scholar]
  11. Ferris D. G. , Nyirjesy P. , Sobel J. D. , Soper D. , Pavletic A. , Litaker M. S. . ( 2002; ). Over-the-counter antifungal drug misuse associated with patient-diagnosed vulvovaginal candidiasis. . Obstet Gynecol 99:, 419–425. [CrossRef] [PubMed]
    [Google Scholar]
  12. Garcia-Vallejo, M. I. (1992). Aceites esenciales de las Lavandulas Ibericas – Ensayo de la quimiotaxonomia. Tesis Doctoral, Universidad Complutense de Madrid.
  13. González-Coloma A. , Martín-Benito D. , Mohamed N. , García-Vallejo M. C. , Soria A. C. . ( 2006; ). Antifeedant effects and chemical composition of essential oils from different populations of Lavandula luisieri L. . Biochem Syst Ecol 34:, 609–616. [CrossRef]
    [Google Scholar]
  14. Joulain D. , König W. A. . ( 1998; ). The Atlas of Spectral Data of Sesquiterpene Hydrocarbons. Hamburg:: E. B. Verlag;.
    [Google Scholar]
  15. Koroch A. R. , Juliani H. R. , Zygadlo J. A. . ( 2007; ). Bioactivity of essential oils and their components. . In Flavours and Fragrances. Chemistry, Bioprocessing and Sustainability, pp. 87–115. Edited by Berger R. G. . . Berlin, Heidelberg:: Springer-Verlag;.
    [Google Scholar]
  16. Marichal P. , Gorrens J. , Van Cutsem J. , Vanden Bossche H. . ( 1986; ). Culture media for the study of the effects of azole derivatives on germ tube formation and hyphal growth of C. albicans . . Mykosen 29:, 76–81.[PubMed] [CrossRef]
    [Google Scholar]
  17. Matos F. , Miguel M. G. , Duarte J. , Venâncio F. , Moiteiro C. , Correia A. I. D. , Figueiredo A. C. , Barroso J. G. , Pedro L. G. . ( 2009; ). Antioxidant capacity of the essential oils from Lavandula luisieri, L. stoechas subsp. lusitanica, L. stoechas subsp. lusitanica x L. luisieri and L. viridis grown in Algarve (Portugal). . JEOR 21:, 327–336.[CrossRef]
    [Google Scholar]
  18. Millard P. J. , Roth B. L. , Thi H. P. , Yue S. T. , Haugland R. P. . ( 1997; ). Development of the FUN-1 family of fluorescent probes for vacuole labeling and viability testing of yeasts. . Appl Environ Microbiol 63:, 2897–2905.[PubMed]
    [Google Scholar]
  19. Mitchell A. P. . ( 1998; ). Dimorphism and virulence in Candida albicans . . Curr Opin Microbiol 1:, 687–692. [CrossRef] [PubMed]
    [Google Scholar]
  20. Moon T. , Wilkinson J. M. , Cavanagh H. M. A. . ( 2006; ). Antibacterial activity of essential oils, hydrosols and plant extracts from Australian grown Lavandula spp. . Int J Aromatherapy 16:, 9–14. [CrossRef]
    [Google Scholar]
  21. Pérez-Parra A. , Muñoz P. , Guinea J. , Martín-Rabadán P. , Guembe M. , Bouza E. . ( 2009; ). Is Candida colonization of central vascular catheters in non-candidemic, non-neutropenic patients an indication for antifungals?. Intensive Care Med 35:, 707–712. [CrossRef] [PubMed]
    [Google Scholar]
  22. Perrucci S. , Macchioni G. , Cioni P. C. , Flamini G. , Morelli I. , Taccini F. . ( 1996; ). The activity of volatile compounds from Lavandula angustifolia against Psoroptes cuniculi . . Phytother Res 10:, 5–8. [CrossRef]
    [Google Scholar]
  23. Pfaller M. A. , Pappas P. G. , Wingard J. R. . ( 2006; ). Invasive fungal pathogens: current epidemiological trends. . Clin Infect Dis 43:, S3–S14. [CrossRef]
    [Google Scholar]
  24. Pina-Vaz C. , Sansonetty F. , Rodrigues A. G. , Costa-Oliveira S. , Tavares C. , Martinez-de-Oliveira J. . ( 2001; ). Cytometric approach for a rapid evaluation of susceptibility of Candida strains to antifungals. . Clin Microbiol Infect 7:, 609–618. [CrossRef] [PubMed]
    [Google Scholar]
  25. Pina-Vaz C. , Gonçalves Rodrigues A. , Pinto E. , Costa-de-Oliveira S. , Tavares C. , Salgueiro L. R. , Cavaleiro C. , Gonçalves M. J. , Martinez-de-Oliveira J. . ( 2004; ). Antifungal actvity of Thymus oils and their major compounds. . J Eur Acad Dermatol 18:, 73–78. [CrossRef]
    [Google Scholar]
  26. Pinto E. , Pina-Vaz C. , Salgueiro L. , Gonçalves M. J. , Costa-de-Oliveira S. , Cavaleiro C. , Palmeira A. , Rodrigues A. , Martinez-de-Oliveira J. . ( 2006; ). Antifungal activity of the essential oil of Thymus pulegioides on Candida, Aspergillus and dermatophyte species. . J Med Microbiol 55:, 1367–1373. [CrossRef] [PubMed]
    [Google Scholar]
  27. Pinto E. , Vale-Silva L. , Cavaleiro C. , Salgueiro L. . ( 2009; ). Antifungal activity of the clove essential oil from Syzygium aromaticum on Candida, Aspergillus and dermatophyte species. . J Med Microbiol 58:, 1454–1462. [CrossRef] [PubMed]
    [Google Scholar]
  28. Ríos J. L. , Recio M. C. . ( 2005; ). Medicinal plants and antimicrobial activity. . J Ethnopharmacol 100:, 80–84. [CrossRef] [PubMed]
    [Google Scholar]
  29. Romani L. . ( 2007; ). Immunity to fungi. . In New Insights in Medical Mycology, pp. 1–18. Edited by Kavanagh K. . . Dordrecht:: Springer;. [CrossRef]
    [Google Scholar]
  30. Salgueiro L. R. , Pinto E. , Gonçalves M. J. , Pina-Vaz C. , Cavaleiro C. , Rodrigues A. G. , Palmeira A. , Tavares C. , Costa-de-Oliveira S. , Martinez-de-Oliveira J. . ( 2004; ). Chemical composition and antifungal activity of the essential oil of Thymbra capitata . . Planta Med 70:, 572–575. [CrossRef] [PubMed]
    [Google Scholar]
  31. Saville S. P. , Lazzell A. L. , Bryant A. P. , Fretzen A. , Monreal A. , Solberg E. O. , Monteagudo C. , Lopez-Ribot J. L. , Milne G. T. . ( 2006; ). Inhibition of filamentation can be used to treat disseminated candidiasis. . Antimicrob Agents Chemother 50:, 3312–3316. [CrossRef] [PubMed]
    [Google Scholar]
  32. Upson T. M. , Andrews S. . ( 2004; ). The Genus Lavandula. Kew, London:: The Royal Botanical Gardens;.
    [Google Scholar]
  33. Zuzarte M. , Gonçalves M. J. , Cavaleiro C. , Dinis A. M. , Canhoto J. M. , Salgueiro L. R. . ( 2009; ). Chemical composition and antifungal activity of the essential oils of Lavandula pedunculata (Miller) Cav. . Chem Biodivers 6:, 1283–1292. [CrossRef] [PubMed]
    [Google Scholar]
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