1887

Abstract

is a major pathogen for immunologically intact humans and its pathogenesis is a model system for evasion of host defences. Antibodies and complement are essential elements of the humoral immune system for prevention and control of infections. The specific hypothesis for the proposed research is that modifies humoral host defences by cleaving IgG that has bound to the bacterial surface, thereby inhibiting opsonophagocytosis. was coated with pooled, purified human IgG and assayed for the shedding of cleaved IgG fragments using ELISA and Western blot analysis. Surface-bound IgG was shed efficiently from in the absence of host blood proteins. Broad-spectrum protease inhibitors prevented cleavage of IgG from the surface, suggesting that staphylococcal proteases are responsible for IgG cleavage. Serine protease inhibitors and cysteine protease inhibitors decreased the cleavage of surface-bound IgG; however, a metalloprotease inhibitor had no effect. Using protease inhibitors to prevent the cleavage of surface-bound IgG increased the binding of complement C3 fragments on the surface of , increased the association with human neutrophils and increased phagocytosis by human neutrophils.

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2011-10-01
2020-01-24
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References

  1. Boles B. R. , Horswill A. R. . ( 2008; ). agr-mediated dispersal of Staphylococcus aureus biofilms. . PLoS Pathog 4:, e1000052. [CrossRef] [PubMed]
    [Google Scholar]
  2. Brown E. J. , Hosea S. W. , Hammer C. H. , Burch C. G. , Frank M. M. . ( 1982; ). A quantitative analysis of the interactions of antipneumococcal antibody and complement in experimental pneumococcal bacteremia. . J Clin Invest 69:, 85–98. [CrossRef] [PubMed]
    [Google Scholar]
  3. Cunnion K. M. , Lee J. C. , Frank M. M. . ( 2001; ). Capsule production and growth phase influence binding of complement to Staphylococcus aureus . . Infect Immun 69:, 6796–6803. [CrossRef] [PubMed]
    [Google Scholar]
  4. Cunnion K. M. , Hair P. S. , Buescher E. S. . ( 2004; ). Cleavage of complement C3b to iC3b on the surface of Staphylococcus aureus is mediated by serum complement factor I. . Infect Immun 72:, 2858–2863. [CrossRef] [PubMed]
    [Google Scholar]
  5. Cunnion K. M. , Buescher E. S. , Hair P. S. . ( 2005; ). Serum complement factor I decreases Staphylococcus aureus phagocytosis. . J Lab Clin Med 146:, 279–286. [CrossRef] [PubMed]
    [Google Scholar]
  6. El-Gilany A. H. , Fathy H. . ( 2009; ). Risk factors of recurrent furunculosis. . Dermatol Online J 15:, 16.[PubMed]
    [Google Scholar]
  7. Fick R. B. Jr , Baltimore R. S. , Squier S. U. , Reynolds H. Y. . ( 1985; ). IgG proteolytic activity of Pseudomonas aeruginosa in cystic fibrosis. . J Infect Dis 151:, 589–598. [CrossRef] [PubMed]
    [Google Scholar]
  8. Hair P. S. , Ward M. D. , Semmes O. J. , Foster T. J. , Cunnion K. M. . ( 2008; ). Staphylococcus aureus clumping factor A binds to complement regulator factor I and increases factor I cleavage of C3b. . J Infect Dis 198:, 125–133. [CrossRef] [PubMed]
    [Google Scholar]
  9. Joiner K. A. , Goldman R. C. , Hammer C. H. , Leive L. , Frank M. M. . ( 1983; ). Studies on the mechanism of bacterial resistance to complement-mediated killing. VI. IgG increases the bactericidal efficiency of C5b-9 for E. coli 0111B4 by acting at a step before C5 cleavage. . J Immunol 131:, 2570–2575.[PubMed]
    [Google Scholar]
  10. Klein E. , Smith D. L. , Laxminarayan R. . ( 2007; ). Hospitalizations and deaths caused by methicillin-resistant Staphylococcus aureus, United States, 1999–2005. . Emerg Infect Dis 13:, 1840–1846.[PubMed] [CrossRef]
    [Google Scholar]
  11. Leijh P. C. , van den Barselaar M. T. , Daha M. R. , van Furth R. . ( 1981; ). Participation of immunoglobulins and complement components in the intracellular killing of Staphylococcus aureus and Escherichia coli by human granulocytes. . Infect Immun 33:, 714–724.[PubMed]
    [Google Scholar]
  12. Loomes L. M. , Kerr M. A. , Senior B. W. . ( 1993; ). The cleavage of immunoglobulin G in vitro and in vivo by a proteinase secreted by the urinary tract pathogen Proteus mirabilis . . J Med Microbiol 39:, 225–232. [CrossRef] [PubMed]
    [Google Scholar]
  13. Molla A. , Kagimoto T. , Maeda H. . ( 1988; ). Cleavage of immunoglobulin G (IgG) and IgA around the hinge region by proteases from Serratia marcescens . . Infect Immun 56:, 916–920.[PubMed]
    [Google Scholar]
  14. Peterson P. K. , Verhoef J. , Sabath L. D. , Quie P. G. . ( 1977; ). Effect of protein A on staphylococcal opsonization. . Infect Immun 15:, 760–764.[PubMed]
    [Google Scholar]
  15. Prokesová L. , Potuzníková B. , Potempa J. , Zikán J. , Radl J. , Hachová L. , Baran K. , Porwit-Bobr Z. , John C. . ( 1992; ). Cleavage of human immunoglobulins by serine proteinase from Staphylococcus aureus . . Immunol Lett 31:, 259–265. [CrossRef] [PubMed]
    [Google Scholar]
  16. Prokesová L. , Potuzníkovà B. , Potempa J. , Zikán J. , Radl J. , Porwit-Bóbr Z. , John C. . ( 1995; ). Cleavage of human immunoglobulins by proteinase from Staphylococcus aureus . . Adv Exp Med Biol 371A:, 613–616.[PubMed]
    [Google Scholar]
  17. Rogers B. A. , Drake A. K. , Spelman D. . ( 2009; ). Methicillin resistant Staphylococcus aureus endocarditis in an Australian tertiary hospital: 1991–2006. . Heart Lung Circ 18:, 208–213. [CrossRef] [PubMed]
    [Google Scholar]
  18. Rooijakkers S. H. , van Wamel W. J. , Ruyken M. , van Kessel K. P. , van Strijp J. A. . ( 2005; ). Anti-opsonic properties of staphylokinase. . Microbes Infect 7:, 476–484. [CrossRef] [PubMed]
    [Google Scholar]
  19. von Pawel-Rammingen U. , Björck L. . ( 2003; ). IdeS and SpeB: immunoglobulin-degrading cysteine proteinases of Streptococcus pyogenes . . Curr Opin Microbiol 6:, 50–55. [CrossRef] [PubMed]
    [Google Scholar]
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