1887

Abstract

We investigated the population structures of faecal in 30 healthy young adults (13 males and 17 females) aged between 20 and 45 years and 29 elderly adults (14 females and 15 males) aged between 65 and 77 years. In all, 1566 strains were typed with the PhPlate system and grouped into biochemical phenotypes (BPTs). Strains with shared BPTs were further typed using randomly amplified polymorphic DNA analysis. Forty-four per cent of the strains were shared between two or more age and gender groups. Elders had a significantly higher (<0.001) number of BPTs (mean±standard error 3.3±0.27) than younger groups (1.82±0.27). Phylogenetic affiliation and virulence-associated genes (VAGs) of the strains showed that more than 80 % of the strains belonging to dominant types belonged to phylogroups B2 and D. Amongst dominant BPTs, phylogenetic group A was significantly associated with females (<0.0001), and elders were more likely to carry group D (<0.0124). Elderly males had a higher prevalence of VAGs than young males (<0.0001) and young females (<0.0005). We conclude that there is a lower prevalence of with uropathogenic properties in healthy young adults than in elders.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.027037-0
2011-05-01
2019-12-11
Loading full text...

Full text loading...

/deliver/fulltext/jmm/60/5/574.html?itemId=/content/journal/jmm/10.1099/jmm.0.027037-0&mimeType=html&fmt=ahah

References

  1. Abraham S. N. , Shin J. S. , Malaviya R. . ( 2001; ). Type 1 fimbriated Escherichia coli-mast cell interactions in cystitis. . J Infect Dis 183: Suppl. 1 S51–S55. [CrossRef] [PubMed]
    [Google Scholar]
  2. Ansaruzzaman M. , Albert M. J. , Nahar S. , Byun R. , Katouli M. , Kühn I. , Möllby R. . ( 2000; ). Clonal groups of enteropathogenic Escherichia coli isolated in case-control studies of diarrhoea in Bangladesh. . J Med Microbiol 49:, 177–185.[PubMed]
    [Google Scholar]
  3. Arthur M. , Johnson C. E. , Rubin R. H. , Arbeit R. D. , Campanelli C. , Kim C. , Steinbach S. , Agarwal M. , Wilkinson R. , Goldstein R. . ( 1989; ). Molecular epidemiology of adhesin and hemolysin virulence factors among uropathogenic Escherichia coli . . Infect Immun 57:, 303–313.[PubMed]
    [Google Scholar]
  4. Bingen E. , Picard B. , Brahimi N. , Mathy S. , Desjardins P. , Elion J. , Denamur E. . ( 1998; ). Phylogenetic analysis of Escherichia coli strains causing neonatal meningitis suggests horizontal gene transfer from a predominant pool of highly virulent B2 group strains. . J Infect Dis 177:, 642–650. [CrossRef] [PubMed]
    [Google Scholar]
  5. Boyd E. F. , Hartl D. L. . ( 1998; ). Chromosomal regions specific to pathogenic isolates of Escherichia coli have a phylogenetically clustered distribution. . J Bacteriol 180:, 1159–1165.[PubMed]
    [Google Scholar]
  6. Chen J. , Griffiths M. W. . ( 1998; ). PCR differentiation of Escherichia coli from other gram-negative bacteria using primers derived from the nucleotide sequences flanking the gene encoding the universal stress protein. . Lett Appl Microbiol 27:, 369–371. [CrossRef] [PubMed]
    [Google Scholar]
  7. Clermont O. , Bonacorsi S. , Bingen E. . ( 2000; ). Rapid and simple determination of the Escherichia coli phylogenetic group. . Appl Environ Microbiol 66:, 4555–4558. [CrossRef] [PubMed]
    [Google Scholar]
  8. Czaja C. A. , Stamm W. E. , Stapleton A. E. , Roberts P. L. , Hawn T. R. , Scholes D. , Samadpour M. , Hultgren S. J. , Hooton T. M. . ( 2009; ). Prospective cohort study of microbial and inflammatory events immediately preceding Escherichia coli recurrent urinary tract infection in women. . J Infect Dis 200:, 528–536. [CrossRef] [PubMed]
    [Google Scholar]
  9. Diard M. , Garry L. , Selva M. , Mosser T. , Denamur E. , Matic I. . ( 2010; ). Pathogenicity-associated islands in extraintestinal pathogenic Escherichia coli are fitness elements involved in intestinal colonization. . J Bacteriol 192:, 4885–4893. [CrossRef] [PubMed]
    [Google Scholar]
  10. Duriez P. , Clermont O. , Bonacorsi S. , Bingen E. , Chaventré A. , Elion J. , Picard B. , Denamur E. . ( 2001; ). Commensal Escherichia coli isolates are phylogenetically distributed among geographically distinct human populations. . Microbiology 147:, 1671–1676.[PubMed]
    [Google Scholar]
  11. Foxman B. . ( 2003; ). Epidemiology of urinary tract infections: incidence, morbidity, and economic costs. . Dis Mon 49:, 53–70. [CrossRef] [PubMed]
    [Google Scholar]
  12. Foxman B. , Zhang L. , Tallman P. , Andree B. C. , Geiger A. M. , Koopman J. S. , Gillespie B. W. , Palin K. A. , Sobel J. D. et al. ( 1997; ). Transmission of uropathogens between sex partners. . J Infect Dis 175:, 989–992. [CrossRef] [PubMed]
    [Google Scholar]
  13. Foxman B. , Barlow R. , D’Arcy H. , Gillespie B. , Sobel J. D. . ( 2000; ). Urinary tract infection: self-reported incidence and associated costs. . Ann Epidemiol 10:, 509–515. [CrossRef] [PubMed]
    [Google Scholar]
  14. Gordon D. M. , Clermont O. , Tolley H. , Denamur E. . ( 2008; ). Assigning Escherichia coli strains to phylogenetic groups: multi-locus sequence typing versus the PCR triplex method. . Environ Microbiol 10:, 2484–2496. [CrossRef] [PubMed]
    [Google Scholar]
  15. Harrington R. D. , Hooton T. M. . ( 2000; ). Urinary tract infection risk factors and gender. . J Gend Specif Med 3:, 27–34.[PubMed]
    [Google Scholar]
  16. Hooton T. M. , Scholes D. , Hughes J. P. , Winter C. , Roberts P. L. , Stapleton A. E. , Stergachis A. , Stamm W. E. . ( 1996; ). A prospective study of risk factors for symptomatic urinary tract infection in young women. . N Engl J Med 335:, 468–474. [CrossRef] [PubMed]
    [Google Scholar]
  17. Johnson J. R. , Stell A. L. . ( 2000; ). Extended virulence genotypes of Escherichia coli strains from patients with urosepsis in relation to phylogeny and host compromise. . J Infect Dis 181:, 261–272. [CrossRef] [PubMed]
    [Google Scholar]
  18. Johnson J. R. , Delavari P. , Kuskowski M. , Stell A. L. . ( 2001; ). Phylogenetic distribution of extraintestinal virulence-associated traits in Escherichia coli . . J Infect Dis 183:, 78–88. [CrossRef] [PubMed]
    [Google Scholar]
  19. Johnson J. R. , Owens K. , Gajewski A. , Kuskowski M. A. . ( 2005; ). Bacterial characteristics in relation to clinical source of Escherichia coli isolates from women with acute cystitis or pyelonephritis and uninfected women. . J Clin Microbiol 43:, 6064–6072. [CrossRef] [PubMed]
    [Google Scholar]
  20. Kaye D. . ( 1980; ). Urinary tract infections in the elderly. . Bull N Y Acad Med 56:, 209–220.[PubMed]
    [Google Scholar]
  21. Landgren M. , Odén H. , Kühn I. , Österlund A. , Kahlmeter G. . ( 2005; ). Diversity among 2481 Escherichia coli from women with community-acquired lower urinary tract infections in 17 countries. . J Antimicrob Chemother 55:, 928–937. [CrossRef] [PubMed]
    [Google Scholar]
  22. Moreno E. , Andreu A. , Pigrau C. , Kuskowski M. A. , Johnson J. R. , Prats G. . ( 2008; ). Relationship between Escherichia coli strains causing acute cystitis in women and the fecal E. coli population of the host. . J Clin Microbiol 46:, 2529–2534. [CrossRef] [PubMed]
    [Google Scholar]
  23. Moreno E. , Johnson J. R. , Pérez T. , Prats G. , Kuskowski M. A. , Andreu A. . ( 2009; ). Structure and urovirulence characteristics of the fecal Escherichia coli population among healthy women. . Microbes Infect 11:, 274–280. [CrossRef] [PubMed]
    [Google Scholar]
  24. Öhman L. , Tullus K. , Katouli M. , Burman L. G. , Stendahl O. . ( 1995; ). Correlation between susceptibility of infants to infections and interaction with neutrophils of Escherichia coli strains causing neonatal and infantile septicemia. . J Infect Dis 171:, 128–133.[PubMed] [CrossRef]
    [Google Scholar]
  25. Ouwehand A. C. , Isolauri E. , Kirjavainen P. V. , Salminen S. J. . ( 1999; ). Adhesion of four Bifidobacterium strains to human intestinal mucus from subjects in different age groups. . FEMS Microbiol Lett 172:, 61–64. [CrossRef] [PubMed]
    [Google Scholar]
  26. Picard B. , Garcia J. S. , Gouriou S. , Duriez P. , Brahimi N. , Bingen E. , Elion J. , Denamur E. . ( 1999; ). The link between phylogeny and virulence in Escherichia coli extraintestinal infection. . Infect Immun 67:, 546–553.[PubMed]
    [Google Scholar]
  27. Ramos N. L. , Saayman M. L. , Chapman T. A. , Tucker J. R. , Smith H. V. , Faoagali J. , Chin J. C. , Brauner A. , Katouli M. . ( 2010; ). Genetic relatedness and virulence gene profiles of Escherichia coli strains isolated from septicaemic and uroseptic patients. . Eur J Clin Microbiol Infect Dis 29:, 15–23. [CrossRef] [PubMed]
    [Google Scholar]
  28. Sannes M. R. , Kuskowski M. A. , Owens K. , Gajewski A. , Johnson J. R. . ( 2004; ). Virulence factor profiles and phylogenetic background of Escherichia coli isolates from veterans with bacteremia and uninfected control subjects. . J Infect Dis 190:, 2121–2128. [CrossRef] [PubMed]
    [Google Scholar]
  29. Saunier K. , Doré J. . ( 2002; ). Gastrointestinal tract and the elderly: functional foods, gut microflora and healthy ageing. . Dig Liver Dis 34: Suppl. 2 S19–S24. [CrossRef] [PubMed]
    [Google Scholar]
  30. Schlager T. A. , Hendley J. O. , Bell A. L. , Whittam T. S. . ( 2002; ). Clonal diversity of Escherichia coli colonizing stools and urinary tracts of young girls. . Infect Immun 70:, 1225–1229. [CrossRef] [PubMed]
    [Google Scholar]
  31. Schlager T. A. , Whittam T. S. , Hendley J. O. , Bhang J. L. , Wobbe C. L. , Stapleton A. . ( 2003; ). Variation in frequency of the virulence-factor gene in Escherichia coli clones colonizing the stools and urinary tracts of healthy prepubertal girls. . J Infect Dis 188:, 1059–1064. [CrossRef] [PubMed]
    [Google Scholar]
  32. Scholes D. , Hooton T. M. , Roberts P. L. , Stapleton A. E. , Gupta K. , Stamm W. E. . ( 2000; ). Risk factors for recurrent urinary tract infection in young women. . J Infect Dis 182:, 1177–1182. [CrossRef] [PubMed]
    [Google Scholar]
  33. Tullus K. , Kühn I. , Ørskov I. , Ørskov F. , Möllby R. . ( 1992; ). The importance of P and type 1 fimbriae for the persistence of Escherichia coli in the human gut. . Epidemiol Infect 108:, 415–421. [CrossRef] [PubMed]
    [Google Scholar]
  34. Waite T. A. , Campbell L. G. . ( 2006; ). Controlling the false discovery rate and increasing statistical power in ecological studies. . Ecoscience 13:, 439–442. [CrossRef]
    [Google Scholar]
  35. Wold A. E. , Thorssén M. , Hull S. , Edén C. S. . ( 1988; ). Attachment of Escherichia coli via mannose- or Galα1→4Galβ-containing receptors to human colonic epithelial cells. . Infect Immun 56:, 2531–2537.[PubMed]
    [Google Scholar]
  36. Wold A. E. , Caugant D. A. , Lidin-Janson G. , de Man P. , Svanborg C. . ( 1992; ). Resident colonic Escherichia coli strains frequently display uropathogenic characteristics. . J Infect Dis 165:, 46–52.[PubMed] [CrossRef]
    [Google Scholar]
  37. Wright K. J. , Seed P. C. , Hultgren S. J. . ( 2007; ). Development of intracellular bacterial communities of uropathogenic Escherichia coli depends on type 1 pili. . Cell Microbiol 9:, 2230–2241. [CrossRef] [PubMed]
    [Google Scholar]
  38. Zhang L. , Foxman B. , Marrs C. . ( 2002; ). Both urinary and rectal Escherichia coli isolates are dominated by strains of phylogenetic group B2. . J Clin Microbiol 40:, 3951–3955. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.027037-0
Loading
/content/journal/jmm/10.1099/jmm.0.027037-0
Loading

Data & Media loading...

Supplements

vol. , part 5, pp. 574-581

RAPD-PCR comparison of the strains. [PDF](170 KB)



PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error