1887

Journal of Medical Microbiology logo

Volume 60, Issue 6

Novel concentration method for the detection of norovirus and sapovirus from water using minute particles of amorphous calcium phosphate Free

Abstract

A novel concentration method using minute particles of amorphous calcium phosphate (ACP) was developed for the detection of caliciviruses including norovirus and sapovirus, agents of human gastroenteritis, from water. In seeding experiments with feline calicivirus (FCV), ACP particles were able to adsorb efficiently the viruses in water, and the FCV-concentrated solution was obtained by dissolution of the virus-adsorbing ACP particles with citric acid after centrifugation. By quantitative real-time RT-PCR, the recovery efficiencies from 300 ml ultrapure water seeded with 10, 10 and >10 copies of FCV were 48, 68 and >100 %, respectively. A comparative study showed that in the addition of viruses at <10 copies, the recovery efficiency of our method was significantly higher (<0.05) than that of the similar calcium flocculation–citrate dissolution method. Using our newly developed method, we successfully detected 2.1×10 copies l of norovirus (each of genogroups I and II) and 5.4×10 copies l of sapovirus (genogroups I, II, IV and V) from river water. The data suggest that our new viral concentration is a rapid, simple, cost efficient and high virus recovery method, and it can be used for routine monitoring of norovirus and sapovirus in water, especially environmental water.

  • Received:
  • Accepted:
  • Published Online:
© 2011 SGM
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.026260-0
2011-06-01
2022-01-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/60/6/780.html?itemId=/content/journal/jmm/10.1099/jmm.0.026260-0&mimeType=html&fmt=ahah

References

  1. Beuret C., Kohler D., Baumgartner A., Lüthi T. M. 2002; Norwalk-like virus sequences in mineral waters: one-year monitoring of three brands. Appl Environ Microbiol 68:1925–1931 [View Article][PubMed]
     [Google Scholar]
  2. Cannon J. L., Papafragkou E., Park G. W., Osborne J., Jaykus L.-A., Vinjé J. 2006; Surrogates for the study of norovirus stability and inactivation in the environment: a comparison of murine norovirus and feline calicivirus. J Food Prot 69:2761–2765[PubMed]
     [Google Scholar]
  3. Chiba S., Sakuma Y., Kogasaka R., Akihara M., Horino K., Nakao T., Fukui S. 1979; An outbreak of gastroenteritis associated with calicivirus in an infant home. J Med Virol 4:249–254 [View Article][PubMed]
     [Google Scholar]
  4. Cliver D. O. 1965; Factors in the membrane filtration of enteroviruses. Appl Microbiol 13:417–431[PubMed]
     [Google Scholar]
  5. Fuhrman J. A., Liang X., Noble R. T. 2005; Rapid detection of enteroviruses in small volumes of natural waters by real-time quantitative reverse transcriptase PCR. Appl Environ Microbiol 71:4523–4530 [View Article][PubMed]
     [Google Scholar]
  6. Gilgen M., Germann D., Lüthy J., Hübner P. 1997; Three-step isolation method for sensitive detection of enterovirus, rotavirus, hepatitis A virus, and small round structured viruses in water samples. Int J Food Microbiol 37:189–199 [View Article][PubMed]
     [Google Scholar]
  7. Haramoto E., Katayama H., Ohgaki S. 2004; Detection of noroviruses in tap water in Japan by means of a new method for concentrating enteric viruses in large volumes of freshwater. Appl Environ Microbiol 70:2154–2160 [View Article][PubMed]
     [Google Scholar]
  8. Haramoto E., Katayama H., Utagawa E., Ohgaki S. 2009; Recovery of human norovirus from water by virus concentration methods. J Virol Methods 160:206–209 [View Article][PubMed]
     [Google Scholar]
  9. Hewitt J., Bell D., Simmons G. C., Rivera-Aban M., Wolf S., Greening G. E. 2007; Gastroenteritis outbreak caused by waterborne norovirus at a New Zealand ski resort. Appl Environ Microbiol 73:7853–7857 [View Article][PubMed]
     [Google Scholar]
  10. Kageyama T., Kojima S., Shinohara M., Uchida K., Fukushi S., Hoshino F. B., Takeda N., Katayama K. 2003; Broadly reactive and highly sensitive assay for Norwalk-like viruses based on real-time quantitative reverse transcription-PCR. J Clin Microbiol 41:1548–1557 [View Article][PubMed]
     [Google Scholar]
  11. Karim M. R., Rhodes E. R., Brinkman N., Wymer L., Fout G. S. 2009; New electropositive filter for concentrating enteroviruses and noroviruses from large volumes of water. Appl Environ Microbiol 75:2393–2399 [View Article][PubMed]
     [Google Scholar]
  12. Katayama H., Shimasaki A., Ohgaki S. 2002; Development of a virus concentration method and its application to detection of enterovirus and norwalk virus from coastal seawater. Appl Environ Microbiol 68:1033–1039 [View Article][PubMed]
     [Google Scholar]
  13. Katzenelson E., Fattal B., Hostovesky T. 1976; Organic flocculation: an efficient second-step concentration method for the detection of viruses in tap water. Appl Environ Microbiol 32:638–639[PubMed]
     [Google Scholar]
  14. Kim S.-H., Cheon D.-S., Kim J.-H., Lee D.-H., Jheong W. H., Heo Y.-J., Chung H.-M., Jee Y., Lee J.-S. 2005; Outbreaks of gastroenteritis that occurred during school excursions in Korea were associated with several waterborne strains of norovirus. J Clin Microbiol 43:4836–4839 [View Article][PubMed]
     [Google Scholar]
  15. Kukkula M., Maunula L., Silvennoinen E., Von Bonsdorff C.-H. 1999; Outbreak of viral gastroenteritis due to drinking water contaminated by Norwalk-like viruses. J Infect Dis 180:1771–1776 [View Article][PubMed]
     [Google Scholar]
  16. Lewis G. D., Metcalf T. G. 1988; Polyethylene glycol precipitation for recovery of pathogenic viruses, including hepatitis A virus and human rotavirus, from oyster, water, and sediment samples. Appl Environ Microbiol 54:1983–1988[PubMed]
     [Google Scholar]
  17. Liu J., Wu Q., Kou X. 2007; Development of a virus concentration method and its application for the detection of noroviruses in drinking water in China. J Microbiol 45:48–52[PubMed]
     [Google Scholar]
  18. Lucena F., Bosch A., Jofre J., Schwartzbrod L. 1985; Identification of viruses isolated from sewage, riverwater and coastal seawater in Barcelona. Water Res 19:1237–1239 [View Article]
     [Google Scholar]
  19. Maunula L., Miettinen I. T., Von Bonsdorff C.-H. 2005; Norovirus outbreaks from drinking water. Emerg Infect Dis 11:1716–1721[PubMed] [CrossRef]
     [Google Scholar]
  20. Mori K., Hayashi Y., Noguchi Y., Kai A., Ohe K., Sakai S., Hara M., Morozumi S. 2006; [Effects of handwashing on feline calicivirus removal as norovirus surrogate]. Kansenshogaku Zasshi 80:496–500 (in Japanese) [PubMed] [CrossRef]
     [Google Scholar]
  21. Nygård K., Torvén M., Ancker C., Knauth S. B., Hedlund K.-O., Giesecke J., Andersson Y., Svensson L. 2003; Emerging genotype (GGIIb) of norovirus in drinking water, Sweden. Emerg Infect Dis 9:1548–1552[PubMed] [CrossRef]
     [Google Scholar]
  22. Oka T., Katayama K., Hansman G. S., Kageyama T., Ogawa S., Wu F.-T., White P. A., Takeda N. 2006; Detection of human sapovirus by real-time reverse transcription-polymerase chain reaction. J Med Virol 78:1347–1353 [View Article][PubMed]
     [Google Scholar]
  23. Parshionikar S. U., Willian-True S., Fout G. S., Robbins D. E., Seys S. A., Cassady J. D., Harris R. 2003; Waterborne outbreak of gastroenteritis associated with a norovirus. Appl Environ Microbiol 69:5263–5268 [View Article][PubMed]
     [Google Scholar]
  24. Preston D. R., Vasudevan T. V., Bitton G., Farrah S. R., Morel J.-L. 1988; Novel approach for modifying microporous filters for virus concentration from water. Appl Environ Microbiol 54:1325–1329[PubMed]
     [Google Scholar]
  25. Shields P. A., Berenfeld S. A., Farrah S. R. 1985; Modified membrane-filter procedure for concentration of enteroviruses from tap water. Appl Environ Microbiol 49:453–455[PubMed]
     [Google Scholar]
  26. Sobsey M. D., Glass J. S. 1980; Poliovirus concentration from tap water with electropositive adsorbent filters. Appl Environ Microbiol 40:201–210[PubMed]
     [Google Scholar]
  27. Sobsey M. D., Glass J. S. 1984; Influence of water quality on enteric virus concentration by microporous filter methods. Appl Environ Microbiol 47:956–960[PubMed]
     [Google Scholar]
  28. Sobsey M. D., Jones B. L. 1979; Concentration of poliovirus from tap water using positively charged microporous filters. Appl Environ Microbiol 37:588–595[PubMed]
     [Google Scholar]
  29. Tokutake Y., Kobayashi M., Akiyama M., Aiki C., Nishio O. 2006; [Food borne outbreak caused by the well water contaminated norovirus]. Kansenshogaku Zasshi 80:238–242 (in Japanese) [PubMed] [CrossRef]
     [Google Scholar]
  30. Yamamoto T., Shimazu T., Endo Y., Shiraishi H., Kageyama T. 2005; Quantitative detection of norovirus in water samples collected from rivers flowing into oyster farms in Miyagi Prefecture, Japan. J Jap Soc Wat Environ 28:515–521 [View Article]
     [Google Scholar]
  31. Yano K., Yoshida Y., Shinkai T., Kaneko M. 1993; A practical method for the concentration of viruses from water using fibriform cellulose and organic coagulant. Water Sci Technol 27:295–298
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.026260-0
Loading
Novel concentration method for the detection of norovirus and sapovirus from water using minute particles of amorphous calcium phosphate
J. Med. Microbiol. 60, 780 (2011); https://doi.org/10.1099/jmm.0.026260-0
/content/journal/jmm/10.1099/jmm.0.026260-0
/content/journal/jmm/10.1099/jmm.0.026260-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error