1887

Abstract

Cationic antimicrobial agents may prevent device-associated infections caused by and . This study reports that the cationic antimicrobial polymer poly(2-(dimethylamino ethyl)methacrylate) (pDMAEMA) was more effective at antagonizing growth of clinical isolates of than of . Importantly, mature biofilms were significantly inactivated by pDMAEMA. The isolates tested were generally more hydrophobic than the isolates and had a less negative charge, although a number of individual and clinical isolates had similar surface hydrophobicity and charge values. Fluorescence spectroscopy and flow cytometry revealed that fluorescently labelled pDMAEMA interacted strongly with compared with . Δ and Δ mutants were less hydrophobic and therefore more susceptible to pDMAEMA than wild-type . Although the different susceptibility of and isolates to pDMAEMA is complex, influenced in part by surface hydrophobicity and charge, these findings nevertheless reveal the potential of pDMAEMA to treat infections.

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2011-07-01
2020-01-22
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References

  1. Adams J. L. , McLean R. J. . ( 1999; ). Impact of rpoS deletion on Escherichia coli biofilms. . Appl Environ Microbiol 65:, 4285–4287.[PubMed]
    [Google Scholar]
  2. Bleyer A. J. . ( 2007; ). Use of antimicrobial catheter lock solutions to prevent catheter-related bacteremia. . Clin J Am Soc Nephrol 2:, 1073–1078. [CrossRef].[PubMed]
    [Google Scholar]
  3. Bütün V. , Armes S. P. , Billingham N. C. . ( 2001; ). Synthesis and aqueous solution properties of near-monodisperse tertiary amine methacrylate homopolymers and diblock copolymers. . Polymer (Guildf) 42:, 5993–6008. [CrossRef]
    [Google Scholar]
  4. Ceri H. , Olson M. E. , Stremick C. , Read R. R. , Morck D. , Buret A. . ( 1999; ). The Calgary Biofilm Device: new technology for rapid determination of antibiotic susceptibilities of bacterial biofilms. . J Clin Microbiol 37:, 1771–1776.[PubMed]
    [Google Scholar]
  5. Christensen G. D. , Bisno A. L. , Parisi J. T. , McLaughlin B. , Hester M. G. , Luther R. W. . ( 1982; ). Nosocomial septicemia due to multiply antibiotic-resistant Staphylococcus epidermidis . . Ann Intern Med 96:, 1–10.[PubMed] [CrossRef]
    [Google Scholar]
  6. CLSI ( 2006; ). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard , , 7th edn.. M7–-A7. Wayne, PA:: Clinical and Laboratory Standards Institute;.
    [Google Scholar]
  7. Conlon K. M. , Humphreys H. , O’Gara J. P. . ( 2002; ). icaR encodes a transcriptional repressor involved in environmental regulation of ica operon expression and biofilm formation in Staphylococcus epidermidis . . J Bacteriol 184:, 4400–4408. [CrossRef].[PubMed]
    [Google Scholar]
  8. Evans E. , Brown M. R. , Gilbert P. . ( 1994; ). Iron chelator, exopolysaccharide and protease production in Staphylococcus epidermidis: a comparative study of the effects of specific growth rate in biofilm and planktonic culture. . Microbiology 140:, 153–157. [CrossRef].[PubMed]
    [Google Scholar]
  9. Haddleton D. M. , Crossman M. C. , Dana B. H. , Duncalf D. J. , Heming A. M. , Kukulj D. , Shooter A. J. . ( 1999; ). Atom transfer polymerization of methyl methacrylate mediated by alkylpyridylmethaninmine type ligands, copper(I) bromide, and alkyl halides in hydrocarbon solution. . Macromolecules 32:, 2110–2119. [CrossRef]
    [Google Scholar]
  10. Handke L. D. , Conlon K. M. , Slater S. R. , Elbaruni S. , Fitzpatrick F. , Humphreys H. , Giles W. P. , Rupp M. E. , Fey P. D. , O’Gara J. P. . ( 2004; ). Genetic and phenotypic analysis of biofilm phenotypic variation in multiple Staphylococcus epidermidis isolates. . J Med Microbiol 53:, 367–374. [CrossRef].[PubMed]
    [Google Scholar]
  11. Heimenz P. C. . ( 1986; ). Chapter 13.. In Principles of Colloid and Surface Chemistry, 2nd edn, vol. 9, pp. 737–790. New York:: Marcel Dekker;.
    [Google Scholar]
  12. Holder I. A. , Boyce S. T. . ( 1994; ). Agar well diffusion assay testing of bacterial susceptibility to various antimicrobials in concentrations non-toxic for human cells in culture. . Burns 20:, 426–429. [CrossRef].[PubMed]
    [Google Scholar]
  13. Horsburgh M. J. , Aish J. L. , White I. J. , Shaw L. , Lithgow J. K. , Foster S. J. . ( 2002; ). σB modulates virulence determinant expression and stress resistance: characterization of a functional rsbU strain derived from Staphylococcus aureus 8325-4. . J Bacteriol 184:, 5457–5467. [CrossRef].[PubMed]
    [Google Scholar]
  14. Iordanescu S. , Surdeanu M. . ( 1976; ). Two restriction and modification systems in Staphylococcus aureus NCTC8325. . J Gen Microbiol 96:, 277–281.[PubMed] [CrossRef]
    [Google Scholar]
  15. Jensen S. O. , Lyon B. R. . ( 2009; ). Genetics of antimicrobial resistance in Staphylococcus aureus . . Future Microbiol 4:, 565–582. [CrossRef].[PubMed]
    [Google Scholar]
  16. Jones D. S. , Adair C. G. , Mawhinney M. W. , Gorman S. P. . ( 1996; ). Standardization and comparison of methods employed for microbial cell surface hydrophobicity and charge determination. . Int J Pharm 131:, 83–89. [CrossRef]
    [Google Scholar]
  17. Jones R. A. , Poniris M. H. , Wilson M. R. . ( 2004; ). pDMAEMA is internalised by endocytosis but does not physically disrupt endosomes. . J Control Release 96:, 379–391. [CrossRef].[PubMed]
    [Google Scholar]
  18. Kinnari T. J. , Esteban J. , Martin-de-Hijas N. Z. , Sánchez-Muñoz O. , Sánchez-Salcedo S. , Colilla M. , Vallet-Regí M. , Gomez-Barrena E. . ( 2009; ). Influence of surface porosity and pH on bacterial adherence to hydroxyapatite and biphasic calcium phosphate bioceramics. . J Med Microbiol 58:, 132–137. [CrossRef].[PubMed]
    [Google Scholar]
  19. Kohler T. , Weidenmaier C. , Peschel A. . ( 2009; ). Wall teichoic acid protects Staphylococcus aureus against antimicrobial fatty acids from human skin. . J Bacteriol 191:, 4482–4484. [CrossRef].[PubMed]
    [Google Scholar]
  20. Kreiswirth B. N. , Löfdahl S. , Betley M. J. , O’Reilly M. , Schlievert P. M. , Bergdoll M. S. , Novick R. P. . ( 1983; ). The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage. . Nature 305:, 709–712. [CrossRef].[PubMed]
    [Google Scholar]
  21. Lewis K. . ( 2001; ). Riddle of biofilm resistance. . Antimicrob Agents Chemother 45:, 999–1007. [CrossRef].[PubMed]
    [Google Scholar]
  22. Limer A. J. , Rullay A. K. , Miguel V. S. , Peinado C. , Keely S. , Fitzpatrick E. , Carrington S. D. , Brayden D. J. , Haddleton D. M. . ( 2006; ). Fluorescently tagged star polymers by living radical polymerisation for mucoadhesion and bioadhesion. . Functional Polymers 66:, 51–64. [CrossRef]
    [Google Scholar]
  23. Lowe A. B. , Vamvakaki M. , Wassall M. A. , Wong L. , Billingham N. C. , Armes S. P. , Lloyd A. W. . ( 2000; ). Well-defined sulfobetaine-based statistical copolymers as potential antibioadherent coatings. . J Biomed Mater Res 52:, 88–94. [CrossRef].[PubMed]
    [Google Scholar]
  24. Mack D. , Siemssen N. , Laufs R. . ( 1992; ). Parallel induction by glucose of adherence and a polysaccharide antigen specific for plastic-adherent Staphylococcus epidermidis: evidence for functional relation to intercellular adhesion. . Infect Immun 60:, 2048–2057.[PubMed]
    [Google Scholar]
  25. National Nosocomial Infections Surveillance System ( 2004; ). National Nosocomial Infections Surveillance (NNIS) System Report, data summary from January 1992 through June 2004, issued October 2004. . Am J Infect Control 32, 470–485.[CrossRef]
    [Google Scholar]
  26. Nostro A. , Marino A. , Blanco A. R. , Cellini L. , Di Giulio M. , Pizzimenti F. , Sudano Roccaro A. , Bisignano G. . ( 2009; ). In vitro activity of carvacrol against staphylococcal preformed biofilm by liquid and vapour contact. . J Med Microbiol 58:, 791–797. [CrossRef].[PubMed]
    [Google Scholar]
  27. O'Neill E. , Pozzi C. , Houston P. , Smyth D. , Humphreys H. , Robinson D. A. , O’Gara J. P. . ( 2007; ). Association between methicillin susceptibility and biofilm regulation in Staphylococcus aureus isolates from device-related infections. . J Clin Microbiol 45:, 1379–1388. [CrossRef].[PubMed]
    [Google Scholar]
  28. Otto M. . ( 2009; ). Staphylococcus epidermidis – the ‘accidental’ pathogen. . Nat Rev Microbiol 7:, 555–567. [CrossRef].[PubMed]
    [Google Scholar]
  29. Peschel A. , Otto M. , Jack R. W. , Kalbacher H. , Jung G. , Götz F. . ( 1999; ). Inactivation of the dlt operon in Staphylococcus aureus confers sensitivity to defensins, protegrins, and other antimicrobial peptides. . J Biol Chem 274:, 8405–8410. [CrossRef].[PubMed]
    [Google Scholar]
  30. Peschel A. , Jack R. W. , Otto M. , Collins L. V. , Staubitz P. , Nicholson G. , Kalbacher H. , Nieuwenhuizen W. F. , Jung G. et al. ( 2001; ). Staphylococcus aureus resistance to human defensins and evasion of neutrophil killing via the novel virulence factor MprF is based on modification of membrane lipids with l-lysine. . J Exp Med 193:, 1067–1076. [CrossRef].[PubMed]
    [Google Scholar]
  31. Pettit R. K. , Weber C. A. , Kean M. J. , Hoffmann H. , Pettit G. R. , Tan R. , Franks K. S. , Horton M. L. . ( 2005; ). Microplate Alamar blue assay for Staphylococcus epidermidis biofilm susceptibility testing. . Antimicrob Agents Chemother 49:, 2612–2617. [CrossRef].[PubMed]
    [Google Scholar]
  32. Qu Y. , Istivan T. S. , Daley A. J. , Rouch D. A. , Deighton M. A. . ( 2009; ). Comparison of various antimicrobial agents as catheter lock solutions: preference for ethanol in eradication of coagulase-negative staphylococcal biofilms. . J Med Microbiol 58:, 442–450. [CrossRef].[PubMed]
    [Google Scholar]
  33. Raafat D. , von Bargen K. , Haas A. , Sahl H. G. . ( 2008; ). Insights into the mode of action of chitosan as an antibacterial compound. . Appl Environ Microbiol 74:, 3764–3773. [CrossRef].[PubMed]
    [Google Scholar]
  34. Rawlinson L. A. , O’Brien P. J. , Brayden D. J. . ( 2010; a). High content analysis of cytotoxic effects of pDMAEMA on human intestinal epithelial and monocyte cultures. . J Control Release 146:, 84–92. [CrossRef].[PubMed]
    [Google Scholar]
  35. Rawlinson L. A. , Ryan S. M. , Mantovani G. , Syrett J. A. , Haddleton D. M. , Brayden D. J. . ( 2010; b). Antibacterial effects of poly(2-(dimethylamino ethyl)methacrylate) against selected Gram-positive and Gram-negative bacteria. . Biomacromolecules 11:, 443–453. [CrossRef].[PubMed]
    [Google Scholar]
  36. Roberts M. E. , Stewart P. S. . ( 2004; ). Modeling antibiotic tolerance in biofilms by accounting for nutrient limitation. . Antimicrob Agents Chemother 48:, 48–52. [CrossRef].[PubMed]
    [Google Scholar]
  37. Robinson D. A. , Enright M. C. . ( 2003; ). Evolutionary models of the emergence of methicillin-resistant Staphylococcus aureus . . Antimicrob Agents Chemother 47:, 3926–3934. [CrossRef].[PubMed]
    [Google Scholar]
  38. Shanks R. M. , Sargent J. L. , Martinez R. M. , Graber M. L. , O’Toole G. A. . ( 2006; ). Catheter lock solutions influence staphylococcal biofilm formation on abiotic surfaces. . Nephrol Dial Transplant 21:, 2247–2255. [CrossRef].[PubMed]
    [Google Scholar]
  39. van der Mei H. C. , Léonard A. J. , Weerkamp A. H. , Rouxhet P. G. , Busscher H. J. . ( 1988; ). Surface properties of Streptococcus salivarius HB and nonfibrillar mutants: measurement of zeta potential and elemental composition with X-ray photoelectron spectroscopy. . J Bacteriol 170:, 2462–2466.[PubMed]
    [Google Scholar]
  40. Walencka E. , Rózalska S. , Sadowska B. , Rózalska B. . ( 2008; ). The influence of Lactobacillus acidophilus-derived surfactants on staphylococcal adhesion and biofilm formation. . Folia Microbiol (Praha) 53:, 61–66. [CrossRef].[PubMed]
    [Google Scholar]
  41. Wang J. , Huang N. , Yang P. , Leng Y. X. , Sun H. , Liu Z. Y. , Chu P. K. . ( 2004; ). The effects of amorphous carbon films deposited on polyethylene terephthalate on bacterial adhesion. . Biomaterials 25:, 3163–3170. [CrossRef].[PubMed]
    [Google Scholar]
  42. Wang H. , Wang L. , Zhang P. , Yuan L. , Yu Q. , Chen H. . ( 2011; ). High antibacterial efficiency of pDMAEMA modified silicon nanowire arrays. . Colloids Surf B Biointerfaces 83:, 355–359. [CrossRef].[PubMed]
    [Google Scholar]
  43. Wilson W. W. , Wade M. M. , Holman S. C. , Champlin F. R. . ( 2001; ). Status of methods for assessing bacterial cell surface charge properties based on zeta potential measurements. . J Microbiol Methods 43:, 153–164. [CrossRef].[PubMed]
    [Google Scholar]
  44. Yang S.-H. , Lee Y.-S. , Lin F.-H. , Yang J.-M. , Chen K.-S. . ( 2007; ). Chitosan/poly(vinyl alcohol) blending hydrogel coating improves the surface characteristics of segmented polyurethane urethral catheters. . J Biomed Mater Res B Appl Biomater 83:, 304–313.[PubMed] [CrossRef]
    [Google Scholar]
  45. Yousefi Rad A. , Ayhan H. , Kisa U. , Pişkin E. . ( 1998; ). Adhesion of different bacterial strains to low-temperature plasma treated biomedical PVC catheter surfaces. . J Biomater Sci Polym Ed 9:, 915–929. [CrossRef].[PubMed]
    [Google Scholar]
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