1887

Abstract

Cationic antimicrobial agents may prevent device-associated infections caused by and . This study reports that the cationic antimicrobial polymer poly(2-(dimethylamino ethyl)methacrylate) (pDMAEMA) was more effective at antagonizing growth of clinical isolates of than of . Importantly, mature biofilms were significantly inactivated by pDMAEMA. The isolates tested were generally more hydrophobic than the isolates and had a less negative charge, although a number of individual and clinical isolates had similar surface hydrophobicity and charge values. Fluorescence spectroscopy and flow cytometry revealed that fluorescently labelled pDMAEMA interacted strongly with compared with . Δ and Δ mutants were less hydrophobic and therefore more susceptible to pDMAEMA than wild-type . Although the different susceptibility of and isolates to pDMAEMA is complex, influenced in part by surface hydrophobicity and charge, these findings nevertheless reveal the potential of pDMAEMA to treat infections.

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2011-07-01
2024-12-04
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References

  1. Adams J. L., McLean R. J. 1999; Impact of rpoS deletion on Escherichia coli biofilms. Appl Environ Microbiol 65:4285–4287[PubMed]
    [Google Scholar]
  2. Bleyer A. J. 2007; Use of antimicrobial catheter lock solutions to prevent catheter-related bacteremia. Clin J Am Soc Nephrol 2:1073–1078 [View Article][PubMed]
    [Google Scholar]
  3. Bütün V., Armes S. P., Billingham N. C. 2001; Synthesis and aqueous solution properties of near-monodisperse tertiary amine methacrylate homopolymers and diblock copolymers. Polymer (Guildf) 42:5993–6008 [View Article]
    [Google Scholar]
  4. Ceri H., Olson M. E., Stremick C., Read R. R., Morck D., Buret A. 1999; The Calgary Biofilm Device: new technology for rapid determination of antibiotic susceptibilities of bacterial biofilms. J Clin Microbiol 37:1771–1776[PubMed]
    [Google Scholar]
  5. Christensen G. D., Bisno A. L., Parisi J. T., McLaughlin B., Hester M. G., Luther R. W. 1982; Nosocomial septicemia due to multiply antibiotic-resistant Staphylococcus epidermidis . Ann Intern Med 96:1–10[PubMed] [CrossRef]
    [Google Scholar]
  6. CLSI 2006 Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard , 7th edn.M7–A7 Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  7. Conlon K. M., Humphreys H., O’Gara J. P. 2002; icaR encodes a transcriptional repressor involved in environmental regulation of ica operon expression and biofilm formation in Staphylococcus epidermidis . J Bacteriol 184:4400–4408 [View Article][PubMed]
    [Google Scholar]
  8. Evans E., Brown M. R., Gilbert P. 1994; Iron chelator, exopolysaccharide and protease production in Staphylococcus epidermidis: a comparative study of the effects of specific growth rate in biofilm and planktonic culture. Microbiology 140:153–157 [View Article][PubMed]
    [Google Scholar]
  9. Haddleton D. M., Crossman M. C., Dana B. H., Duncalf D. J., Heming A. M., Kukulj D., Shooter A. J. 1999; Atom transfer polymerization of methyl methacrylate mediated by alkylpyridylmethaninmine type ligands, copper(I) bromide, and alkyl halides in hydrocarbon solution. Macromolecules 32:2110–2119 [View Article]
    [Google Scholar]
  10. Handke L. D., Conlon K. M., Slater S. R., Elbaruni S., Fitzpatrick F., Humphreys H., Giles W. P., Rupp M. E., Fey P. D., O’Gara J. P. 2004; Genetic and phenotypic analysis of biofilm phenotypic variation in multiple Staphylococcus epidermidis isolates. J Med Microbiol 53:367–374 [View Article][PubMed]
    [Google Scholar]
  11. Heimenz P. C. 1986; Chapter 13.. In Principles of Colloid and Surface Chemistry, 2nd edn. vol. 9 pp. 737–790 New York: Marcel Dekker;
    [Google Scholar]
  12. Holder I. A., Boyce S. T. 1994; Agar well diffusion assay testing of bacterial susceptibility to various antimicrobials in concentrations non-toxic for human cells in culture. Burns 20:426–429 [View Article][PubMed]
    [Google Scholar]
  13. Horsburgh M. J., Aish J. L., White I. J., Shaw L., Lithgow J. K., Foster S. J. 2002; σB modulates virulence determinant expression and stress resistance: characterization of a functional rsbU strain derived from Staphylococcus aureus 8325-4. J Bacteriol 184:5457–5467 [View Article][PubMed]
    [Google Scholar]
  14. Iordanescu S., Surdeanu M. 1976; Two restriction and modification systems in Staphylococcus aureus NCTC8325. J Gen Microbiol 96:277–281[PubMed] [CrossRef]
    [Google Scholar]
  15. Jensen S. O., Lyon B. R. 2009; Genetics of antimicrobial resistance in Staphylococcus aureus . Future Microbiol 4:565–582 [View Article][PubMed]
    [Google Scholar]
  16. Jones D. S., Adair C. G., Mawhinney M. W., Gorman S. P. 1996; Standardization and comparison of methods employed for microbial cell surface hydrophobicity and charge determination. Int J Pharm 131:83–89 [View Article]
    [Google Scholar]
  17. Jones R. A., Poniris M. H., Wilson M. R. 2004; pDMAEMA is internalised by endocytosis but does not physically disrupt endosomes. J Control Release 96:379–391 [View Article][PubMed]
    [Google Scholar]
  18. Kinnari T. J., Esteban J., Martin-de-Hijas N. Z., Sánchez-Muñoz O., Sánchez-Salcedo S., Colilla M., Vallet-Regí M., Gomez-Barrena E. 2009; Influence of surface porosity and pH on bacterial adherence to hydroxyapatite and biphasic calcium phosphate bioceramics. J Med Microbiol 58:132–137 [View Article][PubMed]
    [Google Scholar]
  19. Kohler T., Weidenmaier C., Peschel A. 2009; Wall teichoic acid protects Staphylococcus aureus against antimicrobial fatty acids from human skin. J Bacteriol 191:4482–4484 [View Article][PubMed]
    [Google Scholar]
  20. Kreiswirth B. N., Löfdahl S., Betley M. J., O’Reilly M., Schlievert P. M., Bergdoll M. S., Novick R. P. 1983; The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage. Nature 305:709–712 [View Article][PubMed]
    [Google Scholar]
  21. Lewis K. 2001; Riddle of biofilm resistance. Antimicrob Agents Chemother 45:999–1007 [View Article][PubMed]
    [Google Scholar]
  22. Limer A. J., Rullay A. K., Miguel V. S., Peinado C., Keely S., Fitzpatrick E., Carrington S. D., Brayden D. J., Haddleton D. M. 2006; Fluorescently tagged star polymers by living radical polymerisation for mucoadhesion and bioadhesion. Functional Polymers 66:51–64 [View Article]
    [Google Scholar]
  23. Lowe A. B., Vamvakaki M., Wassall M. A., Wong L., Billingham N. C., Armes S. P., Lloyd A. W. 2000; Well-defined sulfobetaine-based statistical copolymers as potential antibioadherent coatings. J Biomed Mater Res 52:88–94 [View Article][PubMed]
    [Google Scholar]
  24. Mack D., Siemssen N., Laufs R. 1992; Parallel induction by glucose of adherence and a polysaccharide antigen specific for plastic-adherent Staphylococcus epidermidis: evidence for functional relation to intercellular adhesion. Infect Immun 60:2048–2057[PubMed]
    [Google Scholar]
  25. National Nosocomial Infections Surveillance System 2004; National Nosocomial Infections Surveillance (NNIS) System Report, data summary from January 1992 through June 2004, issued October 2004. Am J Infect Control 32470–485 [CrossRef]
    [Google Scholar]
  26. Nostro A., Marino A., Blanco A. R., Cellini L., Di Giulio M., Pizzimenti F., Sudano Roccaro A., Bisignano G. 2009; In vitro activity of carvacrol against staphylococcal preformed biofilm by liquid and vapour contact. J Med Microbiol 58:791–797 [View Article][PubMed]
    [Google Scholar]
  27. O'Neill E., Pozzi C., Houston P., Smyth D., Humphreys H., Robinson D. A., O’Gara J. P. 2007; Association between methicillin susceptibility and biofilm regulation in Staphylococcus aureus isolates from device-related infections. J Clin Microbiol 45:1379–1388 [View Article][PubMed]
    [Google Scholar]
  28. Otto M. 2009; Staphylococcus epidermidis – the ‘accidental’ pathogen. Nat Rev Microbiol 7:555–567 [View Article][PubMed]
    [Google Scholar]
  29. Peschel A., Otto M., Jack R. W., Kalbacher H., Jung G., Götz F. 1999; Inactivation of the dlt operon in Staphylococcus aureus confers sensitivity to defensins, protegrins, and other antimicrobial peptides. J Biol Chem 274:8405–8410 [View Article][PubMed]
    [Google Scholar]
  30. Peschel A., Jack R. W., Otto M., Collins L. V., Staubitz P., Nicholson G., Kalbacher H., Nieuwenhuizen W. F., Jung G. et al. 2001; Staphylococcus aureus resistance to human defensins and evasion of neutrophil killing via the novel virulence factor MprF is based on modification of membrane lipids with l-lysine. J Exp Med 193:1067–1076 [View Article][PubMed]
    [Google Scholar]
  31. Pettit R. K., Weber C. A., Kean M. J., Hoffmann H., Pettit G. R., Tan R., Franks K. S., Horton M. L. 2005; Microplate Alamar blue assay for Staphylococcus epidermidis biofilm susceptibility testing. Antimicrob Agents Chemother 49:2612–2617 [View Article][PubMed]
    [Google Scholar]
  32. Qu Y., Istivan T. S., Daley A. J., Rouch D. A., Deighton M. A. 2009; Comparison of various antimicrobial agents as catheter lock solutions: preference for ethanol in eradication of coagulase-negative staphylococcal biofilms. J Med Microbiol 58:442–450 [View Article][PubMed]
    [Google Scholar]
  33. Raafat D., von Bargen K., Haas A., Sahl H. G. 2008; Insights into the mode of action of chitosan as an antibacterial compound. Appl Environ Microbiol 74:3764–3773 [View Article][PubMed]
    [Google Scholar]
  34. Rawlinson L. A., O’Brien P. J., Brayden D. J. 2010a). High content analysis of cytotoxic effects of pDMAEMA on human intestinal epithelial and monocyte cultures. J Control Release 146:84–92 [View Article][PubMed]
    [Google Scholar]
  35. Rawlinson L. A., Ryan S. M., Mantovani G., Syrett J. A., Haddleton D. M., Brayden D. J. 2010b). Antibacterial effects of poly(2-(dimethylamino ethyl)methacrylate) against selected Gram-positive and Gram-negative bacteria. Biomacromolecules 11:443–453 [View Article][PubMed]
    [Google Scholar]
  36. Roberts M. E., Stewart P. S. 2004; Modeling antibiotic tolerance in biofilms by accounting for nutrient limitation. Antimicrob Agents Chemother 48:48–52 [View Article][PubMed]
    [Google Scholar]
  37. Robinson D. A., Enright M. C. 2003; Evolutionary models of the emergence of methicillin-resistant Staphylococcus aureus . Antimicrob Agents Chemother 47:3926–3934 [View Article][PubMed]
    [Google Scholar]
  38. Shanks R. M., Sargent J. L., Martinez R. M., Graber M. L., O’Toole G. A. 2006; Catheter lock solutions influence staphylococcal biofilm formation on abiotic surfaces. Nephrol Dial Transplant 21:2247–2255 [View Article][PubMed]
    [Google Scholar]
  39. van der Mei H. C., Léonard A. J., Weerkamp A. H., Rouxhet P. G., Busscher H. J. 1988; Surface properties of Streptococcus salivarius HB and nonfibrillar mutants: measurement of zeta potential and elemental composition with X-ray photoelectron spectroscopy. J Bacteriol 170:2462–2466[PubMed]
    [Google Scholar]
  40. Walencka E., Rózalska S., Sadowska B., Rózalska B. 2008; The influence of Lactobacillus acidophilus-derived surfactants on staphylococcal adhesion and biofilm formation. Folia Microbiol (Praha) 53:61–66 [View Article][PubMed]
    [Google Scholar]
  41. Wang J., Huang N., Yang P., Leng Y. X., Sun H., Liu Z. Y., Chu P. K. 2004; The effects of amorphous carbon films deposited on polyethylene terephthalate on bacterial adhesion. Biomaterials 25:3163–3170 [View Article][PubMed]
    [Google Scholar]
  42. Wang H., Wang L., Zhang P., Yuan L., Yu Q., Chen H. 2011; High antibacterial efficiency of pDMAEMA modified silicon nanowire arrays. Colloids Surf B Biointerfaces 83:355–359 [View Article][PubMed]
    [Google Scholar]
  43. Wilson W. W., Wade M. M., Holman S. C., Champlin F. R. 2001; Status of methods for assessing bacterial cell surface charge properties based on zeta potential measurements. J Microbiol Methods 43:153–164 [View Article][PubMed]
    [Google Scholar]
  44. Yang S.-H., Lee Y.-S., Lin F.-H., Yang J.-M., Chen K.-S. 2007; Chitosan/poly(vinyl alcohol) blending hydrogel coating improves the surface characteristics of segmented polyurethane urethral catheters. J Biomed Mater Res B Appl Biomater 83:304–313[PubMed] [CrossRef]
    [Google Scholar]
  45. Yousefi Rad A., Ayhan H., Kisa U., Pişkin E. 1998; Adhesion of different bacterial strains to low-temperature plasma treated biomedical PVC catheter surfaces. J Biomater Sci Polym Ed 9:915–929 [View Article][PubMed]
    [Google Scholar]
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