1887

Abstract

Strongyloidiasis is a neglected disease that is prevalent mainly in tropical and subtropical regions. It is caused by intestinal nematodes of the genus . Due to the rise in worldwide travel, infections are increasingly encountered in non-endemic regions. Diagnosis is hampered by insensitive and laborious detection methods. A universal species real-time PCR was developed with an internal competitive control system. The 95 % limit of detection as determined by probit analysis was one larva per PCR equivalent to 100 larvae per 200 mg stool. The assay proved to be 100 % specific as assessed using a panel of parasites and bacteria and thus might be useful in the diagnostic setting as well as for research.

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2011-04-01
2021-10-20
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