1887

Abstract

The immunogenicity of an infectious laryngotracheitis virus (ILTV) glycoprotein B (gB) plasmid DNA vaccine and the immunoregulatory activity of chicken interleukin-18 (IL-18) were investigated in a challenge model. Two recombinant plasmids, pcDNA3.1/gB (pgB) and pcDNA3.1/IL-18 (pIL-18), containing gB and IL-18 were constructed. Chickens were intramuscularly administered two immunizations 2 weeks apart, and challenged with the virulent CG strain of ILTV 2 weeks later. All animals vaccinated with pgB alone or with a combination of pgB plus pIL-18 developed a specific anti-ILTV ELISA antibody and splenocyte proliferation response. The ratios of CD4 to CD8 T lymphocytes in chickens immunized with pgB plus pIL-18 were significantly higher than in those immunized with pgB alone. Co-injection of pIL-18 significantly increased the production of gamma interferon and IL-2, indicating that IL-18 enhances the T helper 1-dominant immune response. Challenge experiments showed that the morbidity rate in the pgB group (25 %) was significantly higher than that in the pgB plus pIL-18 group (10 %). The mortality rates in the pgB and pgB plus pIL-18 groups were 10 and 0 %, respectively, and the corresponding protection rates were 60 and 80 %. These results indicate that IL-18 may be an effective adjuvant for an ILTV vaccine.

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2011-01-01
2021-01-23
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References

  1. Barouch D. H., Letvin N. L., Seder R. A. 2004; The role of cytokine DNAs as vaccine adjuvants for optimizing cellular immune responses. Immunol Rev 202:266–274 [CrossRef]
    [Google Scholar]
  2. Cantin E. M., Hinton D. R., Chen J., Openshaw H. 1995; Gamma interferon expression during acute and latent nervous system infection by herpes simplex virus type 1. J Virol 69:4898–4905
    [Google Scholar]
  3. Cohen A. D., Boyer J. D., Weiner D. B. 1998; Modulating the immune response to genetic immunization. FASEB J 12:1611–1626
    [Google Scholar]
  4. Dinarello C. A. 1999; Interleukin-18. Methods 19:121–132 [CrossRef]
    [Google Scholar]
  5. Dufour-Zavala L. 2008; Epizootiology of infectious laryngotracheitis and presentation of an industry control program. Avian Dis 52:1–7 [CrossRef]
    [Google Scholar]
  6. Froebel K. S., Pakker N. G., Aiuti F., Bofill M., Choremi-Papadopoulou H., Economidou J., Rabian C., Roos M. T., Ryder L. P. other authors 1999; Standardization and quality assurance of lymphocyte proliferation assays for use in the assessment of immune function. European Concerted Action on Immunological and Virological Markers of HIV Disease Progression. J Immunol Methods 227:85–97 [CrossRef]
    [Google Scholar]
  7. Fuchs W., Veits J., Helferich D., Granzow H., Teifke J. P., Mettenleiter T. C. 2007; Molecular biology of avian infectious laryngotracheitis virus. Vet Res 38:261–279 [CrossRef]
    [Google Scholar]
  8. Gurunathan S., Klinman D. M., Seder R. A. 2000; DNA vaccines: immunology, application, and optimization. Annu Rev Immunol 18:927–974 [CrossRef]
    [Google Scholar]
  9. Landolfo S., Gribaudo G., Angeretti A., Gariglio M. 1995; Mechanisms of viral inhibition by interferons. Pharmacol Ther 65:415–442 [CrossRef]
    [Google Scholar]
  10. Lin Y., Qigai H., Xiaolan Y., Weicheng B., Huanchun C. 2005; The co-administrating of recombinant porcine IL-2 could enhance protective immune responses to PRV inactivated vaccine in pigs. Vaccine 23:4436–4441 [CrossRef]
    [Google Scholar]
  11. Marshall D. J., Rudnick K. A., McCarthy S. G., Mateo L. R., Harris M. C., McCauley C., Snyder L. A. 2006; Interleukin-18 enhances Th1 immunity and tumor protection of a DNA vaccine. Vaccine 24:244–253 [CrossRef]
    [Google Scholar]
  12. Min W., Iillehoj H. S., Burnside J., Weining K. C., Staeheli P., Zhu J. J. 2001; Adjuvant effects of IL-β , IL-2 , IL-8 , IL-15 , IFN-α , IFN-γ , TGF-β4 and lymphotactin on DNA vaccination against Eimeria acervulina . Vaccine 20:267–274 [CrossRef]
    [Google Scholar]
  13. Okamura H., Sakaguchi M., Honda T., Taneno A., Matsuo K., Yamada S. 1994; Construction of recombinant infectious laryngotracheitis virus expressing the LacZ gene of E. coli with thymidine kinase gene. J Vet Med Sci 56:799–801 [CrossRef]
    [Google Scholar]
  14. Salagianni M., Wong K. L., Thomas M. J., Noble A., Kemeny D. M. 2007; An essential role for IL-18 in CD8 T cell-mediated suppression of IgE responses. J Immunol 178:4771–4778 [CrossRef]
    [Google Scholar]
  15. Schnitzlein W. M., Winans R., Ellsworth S., Tripathy D. N. 1995; Generation of thymidine kinase-deficient mutants of infectious laryngotracheitis virus. Virology 209:304–314 [CrossRef]
    [Google Scholar]
  16. Sun H. L., Wang Y. F., Tong G. Z., Zhang P. J., Miao D. Y., Zhi H. D., Wang M., Wang M. 2008; Protection of chickens from Newcastle disease and infectious laryngotracheitis with a recombinant fowlpox virus co-expressing the F, HN genes of Newcastle disease virus and gB gene of infectious laryngotracheitis virus. Avian Dis 52:111–117 [CrossRef]
    [Google Scholar]
  17. Tacket C. O., Roy M. J., Widera G., Swain W. F., Broome S., Edelman R. 1999; Phase I safety and immune response studies of a DNA vaccine encoding hepatitis B surface antigen delivered by a gene delivery device. Vaccine 17:2826–2829 [CrossRef]
    [Google Scholar]
  18. Tang M. J., Wang H. N., Zhou S., Tian G. B. 2008; Enhancement of the immunogenicity of an infectious bronchitis virus DNA vaccine by a bicistronic plasmid encoding nucleocapsid protein and interleukin-2. J Virol Methods 149:42–48 [CrossRef]
    [Google Scholar]
  19. Tong G. Z., Zhang S. J., Wang L., Qiu H. J., Wang Y. F., Wang M. 2001; Protection of chickens from infectious laryngotracheitis with a recombinant fowlpox virus expressing glycoprotein B of infectious laryngotracheitis virus. Avian Pathol 30:143–148 [CrossRef]
    [Google Scholar]
  20. Tovey M. G., Lallemand C. 2010; Adjuvant activity of cytokines. Methods Mol Biol 626:287–309
    [Google Scholar]
  21. Yin R. L., Li C., Yang Z. T., Zhang Y. J., Bai W. L., Li X., Yin R. H., Liu H., Liu S. other authors 2009; Construction and immunogenicity of a DNA vaccine containing clumping factor A of Staphylococcus aureus and bovine IL18. Vet Immunol Immunopathol 132:270–274 [CrossRef]
    [Google Scholar]
  22. Yoon H. A., Aleyas A. G., George J. A., Park S. O., Han Y. W., Lee J. H., Kang H. Y., Kang S. H., Cho J. G., Eo S. K. 2006; Modulation of immune responses induced by DNA vaccine expressing glycoprotein B of pseudorabies virus via coadministration of IFN- γ -associated cytokines. J Interferon Cytokine Res 26:730–738 [CrossRef]
    [Google Scholar]
  23. York J. J., Fahey K. J. 1991; Vaccination with affinity-purified glycoproteins protects chickens against infectious laryngotracheitis herpesvirus. Avian Pathol 20:693–704 [CrossRef]
    [Google Scholar]
  24. Zhang H., Cheng C., Zheng M., Chen J. L., Meng M. J., Zhao Z. Z., Chen Q., Xie Z., Li J. L. other authors 2007; Enhancement of immunity to an Escherichia coli vaccine in mice orally inoculated with a fusion gene encoding porcine interleukin 4 and 6. Vaccine 25:7094–7101 [CrossRef]
    [Google Scholar]
  25. Zhu M., Xu X., Liu H., Liu X., Wang S., Dong F., Yang B., Song G. 2003; Enhancement of DNA vaccine potency against herpes simplex virus 1 by co-administration of an interleukin-18 expression plasmid as a genetic adjuvant. J Med Microbiol 52:223–228 [CrossRef]
    [Google Scholar]
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