@article{mbs:/content/journal/jmm/10.1099/jmm.0.023465-0, author = "Wu, Dejing and Li, Xiangmei and Yang, Yonghong and Zheng, Yaojie and Wang, Chuanqing and Deng, Li and Liu, Lan and Li, Changcong and Shang, Yunxiao and Zhao, Changan and Yu, Sangjie and Shen, Xuzhuang", title = "Superantigen gene profiles and presence of exfoliative toxin genes in community-acquired meticillin-resistant Staphylococcus aureus isolated from Chinese children", journal= "Journal of Medical Microbiology", year = "2011", volume = "60", number = "1", pages = "35-45", doi = "https://doi.org/10.1099/jmm.0.023465-0", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.023465-0", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", keywords = "CC, clonal complex", keywords = "ST, sequence type", keywords = "MRSA, meticillin-resistant Staphylococcus aureus", keywords = "MLST, multilocus sequence typing", keywords = "SCCmec, staphylococcal cassette chromosome mec", keywords = "SAg, superantigen", keywords = "MGE, mobile genetic element", keywords = "CA, community acquired", keywords = "HA, hospital acquired", abstract = "This study aimed to evaluate the distribution of superantigen gene profiles and the presence of exfoliative toxin genes in community-acquired meticillin-resistant Staphylococcus aureus (CA-MRSA) isolated from Chinese children, and simultaneously to assess virulence gene profiles and genetic background. Of the CA-MRSA isolates, 88.9 % (88/99) harboured toxin genes, with sek as the most frequent toxin gene (62.6 %), followed by seq (61.6 %), seb (60.6 %) and sea (35.4 %). The eta gene was detected only in one ST398-IVa-spa t034 strain. The sed and etd genes were not found in any of the isolates tested. A total of 38 virulence genotypes were observed, of which the genotype seb-sek-seq (27.3 %, 24/88) comprised the majority, followed by sea-seb-sek-seq (18.2 %, 16/88). The enterotoxin gene cluster including seg-sei-sem-sen-seo-seu predominated at a rate of 15.1 %. The relationship among toxin genotypes, toxin genes encoding profiles of mobile genetic elements and genetic background was analysed. Among 66 clonal complex (CC) 59 isolates, 87.9 % (58/66) were positive for toxin genes, and 75.8 % (50/66) harboured the toxin gene combination seb-sek-seq. Among seb-sek-seq-positive CC59 strains, 42.0 % (21/50) also carried the sea gene. CC59 corresponded exclusively to accessory gene regulator 1 (agr-1). The data presented here enhance our current knowledge on the virulence determinants of CA-MRSA.", }