1887

Abstract

Our objective was to analyse phenotypic and genetic data of extended-spectrum -lactamase (ESBL)-producing , , and that cause infections in our hospital. Over a 3 year period, 342 randomly selected clinical isolates were tested for ESBL production and evaluated for the presence of the -lactamase genes , and . The antibiotic susceptibilities of these isolates were also determined, and the clonality of the isolates was assessed by PFGE. Based on our analyses, 33/92 (35.9 %) , 31/87 (35.6 %) , 24/80 (30 %) and 17/83 (20.5 %) were identified as ESBL producers. The presence of TEM, SHV or CTX ESBL types was detected in 99/105 (94 %) of the isolates. TLA-1 was not detected in any of the 105 isolates. The dominant ESBL types were (=33), (=31) and (=30). The predominant ESBL identified in and isolates was CTX-M-15, whereas in and the predominant types were SHV-12 and SHV-5, respectively. PFGE genotyping revealed two main genetic patterns in the isolates, types SHV-12 and TEM-1+SHV-5. An outbreak caused by SHV-5+CTX-M-15 was detected. In contrast, most ESBL-producing isolates of and did not have similar PFGE banding patterns and thus were not genetically similar. are a concern in our hospital, especially and . Our results confirm that the CTX-M-15 ESBL type has spread rapidly in the hospital, and thus requires careful monitoring.

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2011-01-01
2020-02-24
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