1887

Abstract

The antibiotic susceptibility of complex strains recovered from the intensive care unit (ICU) of West China Hospital, Sichuan, PR China, from 2006 to 2009 was investigated. The identification of and analysis of carbapenemase-encoding genes and their relationship with IS were performed by PCR. Furthermore, a DiversiLab repetitive extragenic palindromic sequence-based PCR (rep-PCR) microbial typing system and a multilocus sequence typing (MLST) scheme were applied to assess the genetic relationship of the isolates. The results showed that the antibiotic susceptibility of the complex isolates changed and imipenem resistance increased rapidly between 2006 and 2009. The and IS-associated genes were prevalent in the imipenem-resistant isolates. However, the gene was found in only one isolate and no metallo--lactamase genes were detected. The representative multidrug-resistant isolates were identified as one cluster by rep-PCR fingerprinting and belonged to the clonal complex 92 (CC92) according to MLST. These findings indicate a situation of increasing resistance and wide distribution of class D -lactamase genes, especially the acquired IS-associated gene, in isolates in the ICU of West China Hospital, probably caused by expansion of the CC92 clone.

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2011-03-01
2020-01-29
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