1887

Abstract

Two toxigenic isolates recovered from pharyngeal swabs of two patients from the same hospital in Japan during 2001–2002 were characterized by PFGE and ribotyping. Toxin production in different culture media was examined and serological analysis of patient sera was performed. The two isolates could not be distinguished by PFGE; however, their ribotypes were distinguishable. One of the isolates could represent a novel ribotype. Analysis of toxin production in different culture media demonstrated that the two isolates produced varying amounts of the diphtheria toxin. Serological analysis showed a greater than sevenfold increase in the serum antitoxin titre during the course of infection in one patient.

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2010-12-01
2019-10-14
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vol. , part 12, pp. 1497 - 1504

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PCR detection of the PLD-encoding gene in isolates and -activated haemolysis. (a) PCR detection of the PLD gene in clinical isolates, isolate ATCC 51799 and PW8. M, 100 bp DNA ladder marker. (b) -activated haemolysis by clinical isolates. clinical isolates and nontoxigenic strain ATCC 27010 [C7(–)] were streaked close to ATCC 6939 on a sheep blood agar plate. For the clinical isolates, haemolytic zones were visible on the plate.

Alignment of gene products. genes of PW8, 0102 and 0211 are aligned. Asterisks indicate amino acid residues identical to those of PW8.

Primers used in this study.



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