1887

Abstract

To obtain the genotype and antimicrobial susceptibility profiles of isolates from north China, 93 isolates (56 isolates from patients with diarrhoea, 7 isolates from Guillain–Barré syndrome patients and 30 isolates from chicken stools) were selected for multilocus sequence typing (MLST), PFGE and drug resistance testing. A total of 49 sequence types (STs) were identified from the entire panel of 93 isolates. Fifty-six isolates belonged to 14 clonal complexes, while 37 isolates could not be assigned to any known clonal complex. The most frequently observed clonal complexes were ST-21 (11 isolates), ST-353 (10 isolates) and ST-443 (6 isolates). Fifty-three PFGE I patterns were identified among 93 isolates. No erythromycin-, gentamicin- or streptomycin-resistant isolates were found among the 44 strains isolated in 2008. Resistance to nalidixic acid, levofloxacin and ciprofloxacin was observed in 100 % (44/44) of the tested isolates. This study has shown the genetic characteristics of isolates in north China. In addition, overlapping clonal groups were defined by both MLST and PFGE for human and chicken isolates.

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2010-10-01
2019-10-20
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References

  1. Altekruse, S. F., Hunt, J. M., Tollefson, L. K. & Madden, J. M. ( 1994; ). Food and animal sources of human Campylobacter jejuni infection. J Am Vet Med Assoc 204, 57–61.
    [Google Scholar]
  2. Blaser, M. J., Taylor, D. N. & Feldman, R. A. ( 1983; ). Epidemiology of Campylobacter jejuni infections. Epidemiol Rev 5, 157–176.
    [Google Scholar]
  3. Clark, C. G., Bryden, L., Cuff, W. R., Johnson, P. L., Jamieson, F., Ciebin, B. & Wang, G. ( 2005; ). Use of the Oxford multilocus sequence typing protocol and sequencing of the flagellin short variable region to characterize isolates from a large outbreak of waterborne Campylobacter sp. strains in Walkerton, Ontario, Canada. J Clin Microbiol 43, 2080–2091.[CrossRef]
    [Google Scholar]
  4. Dingle, K. E., Colles, F. M., Wareing, D. R., Ure, R., Fox, A. J., Bolton, F. E., Bootsma, H. J., Willems, R. J., Urwin, R. & Maiden, M. C. ( 2001; ). Multilocus sequence typing system for Campylobacter jejuni. J Clin Microbiol 39, 14–23.[CrossRef]
    [Google Scholar]
  5. Fitzgerald, C., Helsel, L. O., Nicholson, M. A., Olsen, S. J., Swerdlow, D. L., Flahart, R., Sexton, J. & Fields, P. I. ( 2001; ). Evaluation of methods for subtyping Campylobacter jejuni during an outbreak involving a food handler. J Clin Microbiol 39, 2386–2390.[CrossRef]
    [Google Scholar]
  6. Goddard, E. A., Lastovica, A. J. & Argent, A. C. ( 1997; ). Campylobacter 0:41 isolation in Guillain-Barré syndrome. Arch Dis Child 76, 526–528.[CrossRef]
    [Google Scholar]
  7. Islam, Z., van Belkum, A., Wagenaar, J. A., Cody, A. J., de Boer, A. G., Tabor, H., Jacobs, B. C., Talukder, K. A. & Endtz, H. P. ( 2009; ). Comparative genotyping of Campylobacter jejuni strains from patients with Guillain-Barré syndrome in Bangladesh. PLoS ONE 4, e7257.[CrossRef]
    [Google Scholar]
  8. Klena, J. D., Parker, C. T., Knibb, K., Ibbitt, J. C., Devane, P. M., Horn, S. T., Miller, W. G. & Konkel, M. E. ( 2004; ). Differentiation of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis by a multiplex PCR developed from the nucleotide sequence of the lipid A gene lpxA. J Clin Microbiol 42, 5549–5557.[CrossRef]
    [Google Scholar]
  9. Kuroki, S., Saida, T., Nukina, M., Haruta, T., Yoshioka, M., Kobayashi, Y. & Nakanishi, H. ( 1993; ). Campylobacter jejuni strains from patients with Guillain-Barré syndrome belong mostly to Penner serogroup 19 and contain beta-N-acetylglucosamine residues. Ann Neurol 33, 243–247.
    [Google Scholar]
  10. Nachamkin, I., Allos, B. M. & Ho, T. ( 1998; ). Campylobacter species and Guillain-Barré syndrome. Clin Microbiol Rev 11, 555–567.
    [Google Scholar]
  11. Rautelin, H. & Hanninen, M. L. ( 1999; ). Comparison of a commercial test for serotyping heat-stable antigens of Campylobacter jejuni with genotyping by pulsed-field gel electrophoresis. J Med Microbiol 48, 617–621.[CrossRef]
    [Google Scholar]
  12. Rees, J. H., Soudain, S. E., Gregson, N. A. & Hughes, R. A. ( 1995; ). Campylobacter jejuni infection and Guillain-Barré syndrome. N Engl J Med 333, 1374–1379.[CrossRef]
    [Google Scholar]
  13. Sheikh, K. A., Ho, T. W., Nachamkin, I., Li, C. Y., Cornblath, D. R., Asbury, A. K., Griffin, J. W. & McKhann, G. M. ( 1998; ). Molecular mimicry in Guillain-Barré syndrome. Ann N Y Acad Sci 845, 307–321.[CrossRef]
    [Google Scholar]
  14. Sopwith, W., Birtles, A., Matthews, M., Fox, A., Gee, S., Painter, M., Regan, M., Syed, Q. & Bolton, E. ( 2006; ). Campylobacter jejuni multilocus sequence types in humans, northwest England, 2003–2004. Emerg Infect Dis 12, 1500–1507.[CrossRef]
    [Google Scholar]
  15. Wang, G., Clark, C. G., Taylor, T. M., Pucknell, C., Barton, C., Price, L., Woodward, D. L. & Rodgers, F. G. ( 2002; ). Colony multiplex PCR assay for identification and differentiation of Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, and C. fetus subsp. fetus. J Clin Microbiol 40, 4744–4747.[CrossRef]
    [Google Scholar]
  16. Wilson, I. G. ( 2002; ). Salmonella and campylobacter contamination of raw retail chickens from different producers: a six year survey. Epidemiol Infect 129, 635–645.[CrossRef]
    [Google Scholar]
  17. Zhang, M., Li, Q., He, L., Meng, F., Gu, Y., Zheng, M., Gong, Y., Wang, P., Ruan, F. & other authors ( 2010; ). Association study between an outbreak of Guillain-Barre syndrome in Jilin, China, and preceding Campylobacter jejuni infection. Foodborne Pathog Dis 7, 913–919.[CrossRef]
    [Google Scholar]
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