1887

Abstract

is an opportunistic human pathogen that causes serious and chronic infections. Many secondary metabolites are secreted throughout its growth, among which phenazine is a known virulence factor and signalling molecule. Phenazine is coordinately controlled by the global regulatory quorum-sensing (QS) systems. Despite the detailed understanding of phenazine biosynthesis pathways in , the regulatory networks are still not fully clear. In the present study, the regulation of the operon () has been investigated. Screening of 5000 transposon mutants revealed 14 interrupted genes with altered expression, including (QteE), which has been identified as a novel regulator of the QS system. Overexpression of in significantly reduced the accumulation of homoserine lactone signals and affected the QS-controlled phenotypes such as the production of pyocyanin, rhamnolipids and LasA protease and swarming motility. Indeed, overexpression of in attenuated its pathogenicity in the potato and fruit fly infection models. These findings suggest that plays an important role in pathogenicity and is part of the regulatory networks controlling phenazine production.

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2011-01-01
2020-01-29
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