1887

Abstract

has been shown to produce more than one capsular polysaccharide (CPS). Analysis of the genome has revealed that the organism contains four CPS operons (I–IV). One of these operons (CPS III) was selected for further study. Comparative sequencing analysis revealed that the genes encoding CPS III are present in and but not in . In this study, CPS III was not found to contribute to the virulence of . Strains containing mutations in CPS III had the same LD value as the wild-type when tested in an animal infection model. Production of CPS III was shown to be induced in water but inhibited in 30 % normal human serum using a reporter fusion assay. Microarray analysis of capsule gene expression in infected hamsters revealed that the genes encoding CPS III were not significantly expressed compared with the genes encoding the previously characterized mannoheptose capsule (CPS I), which is an important virulence factor in . Glycosyl-composition analysis by combined GC/MS indicated that the CPS III genes are involved in the synthesis of a capsule composed of galactose, glucose, mannose and xylose.

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2010-12-01
2019-10-15
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vol. , part 12, pp. 1403 - 1414

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Glycosyl composition analysis of purified CPS III. The peaks for each polysaccharide present are indicated. This figure contains data supplementary to Table 6.

Glycosyl linkage analysis of purified CPS III. The peaks for each linkage are indicated. This figure contains data supplementary to Table 7.

Number of animals that perished following inoculation of strains after 48 h in the Syrian hamster model of melioidosis.



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