1887

Abstract

A multiplex PCR assay was developed for the identification of major types and subtypes of staphylococcal cassette chromosome (SCC) in meticillin-resistant (MRSA) strains. The method uses a novel 9 valent multiplex PCR plus two primer pairs for identification and detection of meticillin resistance. All 389 clinical MRSA isolates from Malaysia and 18 European isolates from the Harmony collection harbouring different SCC types that we tested were correctly characterized by our PCR assay. SCC type III and V were by far the most common types among both hospital- and community-acquired Malaysian MRSA isolates, with an apparent emergence of MRSA harbouring the IVh type.

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2010-10-01
2019-10-22
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