The mouse disease model has the advantage of a broad array of immunological and genetic tools available for basic research. Some studies on transgenic and/or mutant mouse strains as models for experimental leptospirosis have been reported; however, the wider use of such models is hampered by a poor understanding of the outcome of experimental leptospiral infection among the different mouse strains available. Here, the outcome of infection by a virulent strain of Leptospira interrogans serogroup Icterohaemorrhagiae strain Cop was studied in four commonly used wild-type mouse strains: A, CBA, BALB/c and C57BL/6. The end points evaluated in this study were survival, presence of kidney lesions, leptospiral load in kidney samples, microscopic agglutination test titre and anti-leptospiral IgG antibody levels. As expected, none of the mouse strains were susceptible to lethal leptospirosis. However, these strains developed specific pathologies associated with sublethal leptospirosis. The A and C57BL/6 strains exhibited a high leptospiral load in kidney samples and the CBA and C57BL/6 strains developed severe inflammatory lesions, whilst the BALB/c strain proved to be the most resistant to subclinical leptospirosis.
AthanazioD. A.,
SilvaE. F.,
SantosC. S.,
RochaG. M.,
Vannier-SantosM. A.,
McBrideA. J. A.,
KoA. I.,
ReisM. G.2008b; Rattus norvegicus as a model for persistent renal colonization by pathogenic Leptospira interrogans
. Acta Trop 105:176–180[CrossRef]
Barral-NettoM.,
CardosoS. A.,
BarralA.1987; Different patterns of disease in two inbred mouse strains infected with a clone of Leishmania mexicana amazonensis
. Acta Trop 44:5–11
Chagas-JuniorA. D.,
McBrideA. J.,
AthanazioD. A.,
FigueiraC. P.,
MedeirosM. A.,
ReisM. G.,
KoA. I.,
McBrideF. C.2009; An imprint method for detecting leptospires in the hamster model of vaccine-mediated immunity for leptospirosis. J Med Microbiol 58:1632–1637[CrossRef]
ChassinC.,
PicardeauM.,
GoujonJ. M.,
BourhyP.,
QuellardN.,
DarcheS.,
BadellE.,
d'AndonM. F.,
WinterN.other authors2009; TLR4- and TLR2-mediated B cell responses control the clearance of the bacterial pathogen, Leptospira interrogans
. J Immunol 183:2669–2677[CrossRef]
da SilvaJ. B.,
RamosT. M.,
de FrancoM.,
PaivaD.,
HoP. L.,
MartinsE. A.,
PereiraM. M.2009; Chemokines expression during Leptospira interrogans serovar Copenhageni infection in resistant BALB/c and susceptible C3H/HeJ mice. Microb Pathog 47:87–93[CrossRef]
FaineS.1962; The growth of Leptospira australis B in the kidneys of mice in the incipient experimental carrier state. J Hyg (Lond) 60:435–442[CrossRef]
NallyJ. E.,
ChantranuwatC.,
WuX. Y.,
FishbeinM. C.,
PereiraM. M.,
Da SilvaJ. J.,
BlancoD. R.,
LovettM. A.2004; Alveolar septal deposition of immunoglobulin and complement parallels pulmonary hemorrhage in a guinea pig model of severe pulmonary leptospirosis. Am J Pathol 164:1115–1127[CrossRef]
NallyJ. E.,
FishbeinM. C.,
BlancoD. R.,
LovettM. A.2005a; Lethal infection of C3H/HeJ and C3H/SCID mice with an isolate of Leptospira interrogans serovar Copenhageni. Infect Immun 73:7014–7017[CrossRef]
NallyJ. E.,
ChowE.,
FishbeinM. C.,
BlancoD. R.,
LovettM. A.2005b; Changes in lipopolysaccharide O antigen distinguish acute versus chronic Leptospira interrogans infections. Infect Immun 73:3251–3260[CrossRef]
SilvaE. F.,
SantosC. S.,
AthanazioD. A.,
SeyffertN.,
SeixasF. K.,
CerqueiraG. M.,
FagundesM. Q.,
BrodC. S.,
ReisM. G.other authors2008; Characterization of virulence of Leptospira isolates in a hamster model. Vaccine 26:3892–3896[CrossRef]
SpichlerA.,
KoA. I.,
SilvaE. F.,
de BritoT.,
SilvaA. M.,
AthanazioD.,
SilvaC.,
SeguroA.2007; Reversal of renal tubule transporter down-regulation during severe leptospirosis with antimicrobial therapy. Am J Trop Med Hyg 77:1111–1119
ViriyakosolS.,
MatthiasM. A.,
SwancuttM. A.,
KirklandT. N.,
VinetzJ. M.2006; Toll-like receptor 4 protects against lethal Leptospira interrogans serovar Icterohaemorrhagiae infection and contributes to in vivo control of leptospiral burden. Infect Immun 74:887–895[CrossRef]
WHO2003; Serological techniques (MAT and ELISA). In Human Leptospirosis: Guidance for Diagnosis Surveillance and Control pp 63–66 Malta: World Health Organization;