Evaluation of three phenotypic identification systems for clinical isolates of Free

Abstract

spp. have recently been separated from the genus based on their molecular characteristics. It was discovered that can be misidentified as by commonly used phenotypic identification systems. Therefore, this study evaluated the ability of three phenotypic systems to identify compared with the genotypic methods sequence-specific primer PCR (SSP-PCR), 16S rRNA gene sequence analysis using the MicroSeq 500 system16S rDNA bacterial identification system or comparison with GenBank sequences using . The phenotypic systems examined in this study were the VITEK 2 GN ID card, the MicroScan Neg Combo 32 panel and API 20E. The SSP-PCR panel was able to distinguish the reference strain from other spp. and . Of the 27 isolates identified as by SSP-PCR, VITEK 2 identified all of them as MicroScan and API identified 25 isolates (92.6 %) and 24 isolates (88.9 %) as , respectively. These isolates were ornithine decarboxylase (ODC) negative in all three phenotypic systems. MicroSeq 500 identified 24 isolates (88.9 %) as , whereas GenBank identification was heterogeneous. Of the 68 isolates identified as by SSP-PCR, 66 isolates (97.1 %) were identified as by VITEK 2, MicroScan and API. MicroScan and API require additional biochemical tests to differentiate between ODC-negative and .

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2011-04-01
2024-03-28
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