1887

Abstract

spp. have recently been separated from the genus based on their molecular characteristics. It was discovered that can be misidentified as by commonly used phenotypic identification systems. Therefore, this study evaluated the ability of three phenotypic systems to identify compared with the genotypic methods sequence-specific primer PCR (SSP-PCR), 16S rRNA gene sequence analysis using the MicroSeq 500 system16S rDNA bacterial identification system or comparison with GenBank sequences using . The phenotypic systems examined in this study were the VITEK 2 GN ID card, the MicroScan Neg Combo 32 panel and API 20E. The SSP-PCR panel was able to distinguish the reference strain from other spp. and . Of the 27 isolates identified as by SSP-PCR, VITEK 2 identified all of them as MicroScan and API identified 25 isolates (92.6 %) and 24 isolates (88.9 %) as , respectively. These isolates were ornithine decarboxylase (ODC) negative in all three phenotypic systems. MicroSeq 500 identified 24 isolates (88.9 %) as , whereas GenBank identification was heterogeneous. Of the 68 isolates identified as by SSP-PCR, 66 isolates (97.1 %) were identified as by VITEK 2, MicroScan and API. MicroScan and API require additional biochemical tests to differentiate between ODC-negative and .

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.020768-0
2011-04-01
2019-09-18
Loading full text...

Full text loading...

/deliver/fulltext/jmm/60/4/492.html?itemId=/content/journal/jmm/10.1099/jmm.0.020768-0&mimeType=html&fmt=ahah

References

  1. Bagley, S. T., Seidler, R. J. & Brenner, D. J. ( 1981; ). Klebsiella planticola sp. nov.: a new species of Enterobacteriaceae found primarily in nonclinical environments. Curr Microbiol 6, 105–109.[CrossRef]
    [Google Scholar]
  2. Boye, K. & Hansen, D. S. ( 2003; ). Sequencing of 16S rDNA of Klebsiella: taxonomic relations within the genus and to other Enterobacteriaceae. Int J Med Microbiol 292, 495–503.[CrossRef]
    [Google Scholar]
  3. Drancourt, M., Bollet, C., Carlioz, A., Martelin, R., Gayral, J. P. & Raoult, D. ( 2000; ). 16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates. J Clin Microbiol 38, 3623–3630.
    [Google Scholar]
  4. Drancourt, M., Bollet, C., Carta, A. & Rousselier, P. ( 2001; ). Phylogenetic analyses of Klebsiella species delineate Klebsiella and Raoultella gen. nov., with description of Raoultella ornithinolytica comb. nov., Raoultella terrigena comb. nov. and Raoultella planticola comb. nov. Int J Syst Evol Microbiol 51, 925–932.[CrossRef]
    [Google Scholar]
  5. Ehrhardt, A. F., Sanders, C. C., Thomson, K. S., Watanakunakorn, C. & Trujillano-Martin, I. ( 1993; ). Emergence of resistance to imipenem in Enterobacter isolates masquerading as Klebsiella pneumoniae during therapy with imipenem/cilastatin. Clin Infect Dis 17, 120–122.[CrossRef]
    [Google Scholar]
  6. Granier, S. A., Plaisance, L., Leflon-Guibout, V., Lagier, E., Morand, S., Goldstein, F. W. & Nicolas-Chanoine, M. H. ( 2003; ). Recognition of two genetic groups in the Klebsiella oxytoca taxon on the basis of chromosomal β-lactamase and housekeeping gene sequences as well as ERIC-1R PCR typing. Int J Syst Evol Microbiol 53, 661–668.[CrossRef]
    [Google Scholar]
  7. Izard, D., Ferragut, C., Gavini, F., Kersters, K., De Ley, J. & Leclerc, H. ( 1981; ). Klebsiella terrigena, a new species from soil and water. Int J Syst Bacteriol 31, 116–127.[CrossRef]
    [Google Scholar]
  8. Kanki, M., Yoda, T., Tsukamoto, T. & Shibata, T. ( 2002; ). Klebsiella pneumoniae produces no histamine: Raoultella planticola and Raoultella ornithinolytica strains are histamine producers. Appl Environ Microbiol 68, 3462–3466.[CrossRef]
    [Google Scholar]
  9. Kovtunovych, G., Lytvynenko, T., Negrutska, V., Lar, O., Brisse, S. & Kozyrovska, N. ( 2003; ). Identification of Klebsiella oxytoca using a specific PCR assay targeting the polygalacturonase pehX gene. Res Microbiol 154, 587–592.[CrossRef]
    [Google Scholar]
  10. Krawczyk, B., Naumiuk, L., Lewandowski, K., Baraniak, A., Gniadkowski, M., Samet, A. & Kur, J. ( 2003; ). Evaluation and comparison of random amplification of polymorphic DNA, pulsed-field gel electrophoresis and ADSRRS-fingerprinting for typing Serratia marcescens outbreaks. FEMS Immunol Med Microbiol 38, 241–248.[CrossRef]
    [Google Scholar]
  11. Mollet, C., Drancourt, M. & Raoult, D. ( 1997; ). rpoB sequence analysis as a novel basis for bacterial identification. Mol Microbiol 26, 1005–1011.[CrossRef]
    [Google Scholar]
  12. Monnet, D. & Freney, J. ( 1994; ). Method for differentiating Klebsiella planticola and Klebsiella terrigena from other Klebsiella species. J Clin Microbiol 32, 1121–1122.
    [Google Scholar]
  13. Monnet, D., Freney, J., Brun, Y., Boeufgras, J. M. & Fleurette, J. ( 1991; ). Difficulties in identifying Klebsiella strains of clinical origin. Zentralbl Bakteriol 274, 456–464.[CrossRef]
    [Google Scholar]
  14. Sakazaki, R., Tamura, K., Kosako, Y. & Yoshizaki, E. ( 1989; ). Klebsiella ornithinolytica sp. nov., formerly known as ornithine-positive Klebsiella oxytoca. Curr Microbiol 18, 201–206.[CrossRef]
    [Google Scholar]
  15. Walckenaer, E., Poirel, L., Leflon-Guibout, V., Nordmann, P. & Nicolas-Chanoine, M. H. ( 2004; ). Genetic and biochemical characterization of the chromosomal class A β-lactamases of Raoultella (formerly Klebsiella) planticola and Raoultella ornithinolytica. Antimicrob Agents Chemother 48, 305–312.[CrossRef]
    [Google Scholar]
  16. Walckenaer, E., Leflon-Guibout, V. & Nicolas-Chanoine, M. H. ( 2008; ). How to identify Raoultella spp. including R. ornithinolytica isolates negative for ornithine decarboxylase? The reliability of the chromosomal bla gene. J Microbiol Methods 75, 405–410.[CrossRef]
    [Google Scholar]
  17. Westbrook, G. L., O'Hara, C. M., Roman, S. B. & Miller, J. M. ( 2000; ). Incidence and identification of Klebsiella planticola in clinical isolates with emphasis on newborns. J Clin Microbiol 38, 1495–1497.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.020768-0
Loading
/content/journal/jmm/10.1099/jmm.0.020768-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error