1887

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Volume 59, Issue 9

Molecular characterization of -related strains based on the sequence analysis of , internal transcribed spacer and 16S rRNA genes Free

Abstract

We investigated the molecular epidemiological features of 94 -related strains, isolated from Korean patients, using sequence analysis targeting 3 independent chronometer molecules, , the internal transcribed spacer 1 region and the 16S rRNA gene. By collective consideration of these three gene-based approaches, the 94 strains were divided into 5 groups (INT1, INT2, INT3, INT4 and INT5). The frequencies of genotype INT1, 2, 3, 4 and 5 in the 94 isolates were 57.4 % (54), 27.7 % (26), 6.4 % (6), 5.3 % (5) and 3.2 % (3), respectively. When correlations between genotypes and clinical parameters (age, sex, radiological type and the presence of a cavity) were analysed in 78 patients with non-tuberculous mycobacteria pulmonary diseases, no relationships were observed with respect to age, sex and radiological type, but genotype and the presence of a cavity tended to be related (=0.051).

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Keyword(s): ITS1, internal transcribed spacer 1 , MAC, Mycobacterium avium complex , NTM, non-tuberculous mycobacteria , PRA, PCR restriction analysis and SNP, single nucleotide polymorphism
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2010-09-01
2024-04-20
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Molecular characterization of Mycobacterium intracellulare-related strains based on the sequence analysis of hsp65, internal transcribed spacer and 16S rRNA genes
J. Med. Microbiol. 59, 1037 (2010); https://doi.org/10.1099/jmm.0.020727-0
/content/journal/jmm/10.1099/jmm.0.020727-0
/content/journal/jmm/10.1099/jmm.0.020727-0
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