1887

Abstract

We investigated the molecular epidemiological features of 94 -related strains, isolated from Korean patients, using sequence analysis targeting 3 independent chronometer molecules, , the internal transcribed spacer 1 region and the 16S rRNA gene. By collective consideration of these three gene-based approaches, the 94 strains were divided into 5 groups (INT1, INT2, INT3, INT4 and INT5). The frequencies of genotype INT1, 2, 3, 4 and 5 in the 94 isolates were 57.4 % (54), 27.7 % (26), 6.4 % (6), 5.3 % (5) and 3.2 % (3), respectively. When correlations between genotypes and clinical parameters (age, sex, radiological type and the presence of a cavity) were analysed in 78 patients with non-tuberculous mycobacteria pulmonary diseases, no relationships were observed with respect to age, sex and radiological type, but genotype and the presence of a cavity tended to be related (=0.051).

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.020727-0
2010-09-01
2021-02-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/59/9/1037.html?itemId=/content/journal/jmm/10.1099/jmm.0.020727-0&mimeType=html&fmt=ahah

References

  1. Alvarez J., García I. G., Aranaz A., Bezos J., Romero B., de Juan L., Mateos A., Gómez-Mampaso E., Domínguez L. 2008; Genetic diversity of Mycobacterium avium isolates recovered from clinical samples and from the environment: molecular characterization for diagnostic purposes. J Clin Microbiol 46:1246–1251 [CrossRef]
    [Google Scholar]
  2. Bai G. H., Park K. S., Kim S. J. 1993; Clinically isolated mycobacteria other than Mycobacterium tuberculosis from 1980 to 1990 in Korea. J Korean Soc Microbiol 28:1–5
    [Google Scholar]
  3. Bang D., Herlin T., Stegger M., Andersen A. B., Torkko P., Tortoli E., Thomsen V. O. 2008; Mycobacterium arosiense sp. nov., a slowly growing, scotochromogenic species causing osteomyelitis in an immunocompromised child. Int J Syst Evol Microbiol 58:2398–2402 [CrossRef]
    [Google Scholar]
  4. Ben Salah I., Cayrou C., Raoult D., Drancourt M. 2009; Mycobacterium marseillense sp. nov., Mycobacterium timonense sp. nov. and Mycobacterium bouchedurhonense sp. nov., members of the Mycobacterium avium complex. Int J Syst Evol Microbiol 59:2803–2808 [CrossRef]
    [Google Scholar]
  5. Böddinghaus B., Wolters J., Heikens W., Böttger E. C. 1990; Phylogenetic analysis and identification of different serovars of Mycobacterium intracellulare at the molecular level. FEMS Microbiol Lett 58:197–203
    [Google Scholar]
  6. Falkinham J. O. 1996; Epidemiology of nontuberculous mycobacteria. Clin Microbiol Rev 9:177–215
    [Google Scholar]
  7. Frothingham R., Wilson K. H. 1993; Sequence-based differentiation of strains in the Mycobacterium avium complex. J Bacteriol 175:2818–2825
    [Google Scholar]
  8. Griffith D. E., Aksamit T., Brown-Elliott B. A., Catanzaro A., Daley C., Gordin F., Holland S. M., Horsburgh R., Huitt G. other authors 2007; An official ATS/IDSA statement: diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases. Am J Respir Crit Care Med 175:367–416 [CrossRef]
    [Google Scholar]
  9. Han X. Y., Tarrand J. J., Infante R., Jacobson K. L., Truong M. 2005; Clinical significance and epidemiologic analyses of Mycobacterium avium and Mycobacterium intracellulare among patients without AIDS. J Clin Microbiol 43:4407–4412 [CrossRef]
    [Google Scholar]
  10. Inderlied C. B., Kemper C. A., Bermudez L. E. 1993; The Mycobacterium avium complex. Clin Microbiol Rev 6:266–310
    [Google Scholar]
  11. Kim H., Kim S. H., Shim T. S., Kim M. N., Bai G. H., Park Y. G., Lee S. H., Cha C. Y., Kook Y. H., Kim B. J. 2005; PCR restriction fragment length polymorphism analysis (PRA)-algorithm targeting 644 bp heat shock protein 65 ( hsp65 ) gene for differentiation of Mycobacterium spp. J Microbiol Methods 62:199–209 [CrossRef]
    [Google Scholar]
  12. Kim H. J., Mun H. S., Kim H., Oh E. J., Ha Y., Bai G. H., Park Y. G., Cha C. Y., Kook Y. H., Kim B. J. 2006; Differentiation of mycobacterial species by hsp65 duplex PCR followed by duplex-PCR-based restriction analysis and direct sequencing. J Clin Microbiol 44:3855–3862 [CrossRef]
    [Google Scholar]
  13. Koh W. J., Kwon O. J., Lee K. S. 2005; Diagnosis and treatment of nontuberculous mycobacterial pulmonary diseases: a Korean perspective. J Korean Med Sci 20:913–925 [CrossRef]
    [Google Scholar]
  14. Koh W. J., Kwon O. J., Jeon K., Kim T. S., Lee K. S., Park Y. K., Bai G. H. 2006; Clinical significance of nontuberculous mycobacteria isolated from respiratory specimens in Korea. Chest 129:341–348 [CrossRef]
    [Google Scholar]
  15. Kumar S., Dudley J., Nei M., Tamura K. 2008; mega: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 9:299–306 [CrossRef]
    [Google Scholar]
  16. Lee H., Park H. J., Cho S. N., Bai G. H., Kim S. J. 2000; Species identification of mycobacteria by PCR-restriction fragment length polymorphism of the rpoB gene. J Clin Microbiol 38:2966–2971
    [Google Scholar]
  17. Mijs W., de Haas P., Rossau R., Van der Laan T., Rigouts L., Portaels F., van Soolingen D. 2002; Molecular evidence to support a proposal to reserve the designation Mycobacterium avium subsp. avium for bird-type isolates and ‘ M.avium subsp. hominissuis ’ for the human/porcine type of M. avium . Int J Syst Evol Microbiol 52:1505–1518 [CrossRef]
    [Google Scholar]
  18. Mun H. S., Kim H. J., Oh E. J., Kim H., Park Y. G., Bai G. H., Do J., Cha C. Y., Kook Y. H., Kim B. J. 2007; Direct application of Ava II PCR restriction fragment length polymorphism analysis ( Ava II PRA) targeting 644 bp heat shock protein 65 ( hsp65 ) gene to sputum samples. Microbiol Immunol 51:105–110 [CrossRef]
    [Google Scholar]
  19. Murcia M. I., Tortoli E., Menendez M. C., Palenque E., Garcia M. J. 2006; Mycobacterium colombiense sp. nov., a novel member of the Mycobacterium avium complex and description of MAC-X as a new ITS genetic variant. Int J Syst Evol Microbiol 56:2049–2054 [CrossRef]
    [Google Scholar]
  20. Ryoo S. W., Shin S., Shim M. S., Park Y. S., Lew W. J., Park S. N., Park Y. K., Kang S. 2008; Spread of nontuberculous mycobacteria from 1993 to 2006 in Koreans. J Clin Lab Anal 22:415–420 [CrossRef]
    [Google Scholar]
  21. Schweickert B., Goldenberg O., Richter E., Göbel U. B., Petrich A., Buchholz P., Moter A. 2008; Occurrence and clinical relevance of Mycobacterium chimaera sp. nov., Germany. Emerg Infect Dis 14:1443–1446 [CrossRef]
    [Google Scholar]
  22. Semret M., Turenne C. Y., de Haas P., Collins D. M., Behr M. A. 2006; Differentiating host-associated variants of Mycobacterium avium by PCR for detection of large sequence polymorphisms. J Clin Microbiol 44:881–887 [CrossRef]
    [Google Scholar]
  23. Stout J. E., Hopkins G. W., McDonald J. R., Quinn A., Hamilton C. D., Reller L. B., Frothingham R. 2008; Association between 16S–23S internal transcribed spacer sequence groups of Mycobacterium avium complex and pulmonary disease. J Clin Microbiol 46:2790–2793 [CrossRef]
    [Google Scholar]
  24. Tortoli E., Rindi L., Garcia M. J., Chiaradonna P., Dei R., Garzelli C., Kroppenstedt R. M., Lari N., Mattei R. other authors 2004; Proposal to elevate the genetic variant MAC-A, included in the Mycobacterium avium complex, to species rank as Mycobacterium chimaera sp. nov. Int J Syst Evol Microbiol 54:1277–1285 [CrossRef]
    [Google Scholar]
  25. Turenne C. Y., Semret M., Cousins D. V., Collins D. M., Behr M. A. 2006; Sequencing of hsp65 distinguishes among subsets of the Mycobacterium avium complex. J Clin Microbiol 44:433–440 [CrossRef]
    [Google Scholar]
  26. Turenne C. Y., Wallace R. Jr, Behr M. A. 2007; Mycobacterium avium in the postgenomic era. Clin Microbiol Rev 20:205–229 [CrossRef]
    [Google Scholar]
  27. Turenne C. Y., Collins D. M., Alexander D. C., Behr M. A. 2008; Mycobacterium avium subsp. paratuberculosis and M. avium subsp. avium are independently evolved pathogenic clones of a much broader group of M. avium organisms. J Bacteriol 190:2479–2487 [CrossRef]
    [Google Scholar]
  28. van Ingen J., Boeree M. J., Kösters K., Wieland A., Tortoli E., Dekhuijzen P. N., van Soolingen D. 2009; Proposal to elevate Mycobacterium avium complex ITS sequevar MAC-Q to Mycobacterium vulneris sp. nov. Int J Syst Evol Microbiol 59:2277–2282 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.020727-0
Loading
/content/journal/jmm/10.1099/jmm.0.020727-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error