1887

Abstract

Enterococci are important nosocomial pathogens with multiple intrinsic and acquired resistances to antibiotics. In the past, the majority of infections were caused by ; however, an increase in clinical isolates has been observed in recent years. The enterococcal surface protein (Esp) is expressed on the surface of most clinical isolates and has been shown to be involved in biofilm formation. Here, E1162 and its previously created insertion-deletion mutant of the gene, E1162Δ, were compared in a mouse bacteraemia model. Anti-Esp serum was tested for its capacity to mediate opsonophagocytic killing of E1162 and to protect against bacteraemia. The inactivation of attenuated virulence with reduced numbers of bacteria recovered from the kidneys in animals infected with the mutant compared to the wild-type strain (=0.035). Passive immunization with rabbit polyclonal serum raised against the recombinant N-terminal Esp protein did not protect mice against bacteraemia (>0.05). In contrast, mice passively immunized with polyclonal antiserum raised against lipoteichoic acid (LTA) from had lower numbers of E1162 in the blood compared to mice immunized with normal rabbit serum. These results suggest that Esp contributes to persistence in the host. However, in contrast to LTA, Esp does not seem to be a target for protective antibodies in strain E1162 in mouse bacteraemia.

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2010-09-01
2019-10-18
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References

  1. Arias, C. A. & Murray, B. E. ( 2009; ). Antibiotic-resistant bugs in the 21st century – a clinical super-challenge. N Engl J Med 360, 439–443.[CrossRef]
    [Google Scholar]
  2. Baldassarri, L., Bertuccini, L., Ammendolia, M. G., Gherardi, G. & Creti, R. ( 2001; ). Variant esp gene in vancomycin-sensitive Enterococcus faecium. Lancet 357, 1802
    [Google Scholar]
  3. Burnie, J., Carter, T., Rigg, G., Hodgetts, S., Donohoe, M. & Matthews, R. ( 2002; ). Identification of ABC transporters in vancomycin-resistant Enterococcus faecium as potential targets for antibody therapy. FEMS Immunol Med Microbiol 33, 179–189.[CrossRef]
    [Google Scholar]
  4. Heikens, E., Bonten, M. J. & Willems, R. J. ( 2007; ). Enterococcal surface protein Esp is important for biofilm formation of Enterococcus faecium E1162. J Bacteriol 189, 8233–8240.[CrossRef]
    [Google Scholar]
  5. Heikens, E., Leendertse, M., Wijnands, L., van Luit-Asbroek, M., Bonten, M. J., Van Der Poll, T. & Willems, R. J. ( 2009; ). Enterococcal surface protein Esp is not essential for cell adhesion and intestinal colonization of Enterococcus faecium in mice. BMC Microbiol 9, 19 [CrossRef]
    [Google Scholar]
  6. Huebner, J., Wang, Y., Krueger, W. A., Madoff, L. C., Martirosian, G., Boisot, S., Goldmann, D. A., Kasper, D. L., Tzianabos, A. O. & other authors ( 1999; ). Isolation and chemical characterization of a capsular polysaccharide antigen shared by clinical isolates of Enterococcus faecalis and vancomycin-resistant Enterococcus faecium. Infect Immun 67, 1213–1219.
    [Google Scholar]
  7. Huebner, J., Quaas, A., Krueger, W. A., Goldmann, D. A. & Pier, G. B. ( 2000; ). Prophylactic and therapeutic efficacy of antibodies to a capsular polysaccharide shared among vancomycin-sensitive and -resistant enterococci. Infect Immun 68, 4631–4636.[CrossRef]
    [Google Scholar]
  8. Hufnagel, M., Koch, S., Creti, R., Baldassarri, L. & Huebner, J. ( 2004; ). A putative sugar-binding transcriptional regulator in a novel gene locus in Enterococcus faecalis contributes to production of biofilm and prolonged bacteremia in mice. J Infect Dis 189, 420–430.[CrossRef]
    [Google Scholar]
  9. Jett, B. D., Huycke, M. M. & Gilmore, M. S. ( 1994; ). Virulence of enterococci. Clin Microbiol Rev 7, 462–478.
    [Google Scholar]
  10. Koch, S., Hufnagel, M., Theilacker, C. & Huebner, J. ( 2004; ). Enterococcal infections: host response, therapeutic, and prophylactic possibilities. Vaccine 22, 822–830.[CrossRef]
    [Google Scholar]
  11. Kristich, C. J., Li, Y. H., Cvitkovitch, D. G. & Dunny, G. M. ( 2004; ). Esp-independent biofilm formation by Enterococcus faecalis. J Bacteriol 186, 154–163.[CrossRef]
    [Google Scholar]
  12. Lachenauer, C. S. & Madoff, L. C. ( 1996; ). A protective surface protein from type V group B streptococci shares N-terminal sequence homology with the alpha C protein. Infect Immun 64, 4255–4260.
    [Google Scholar]
  13. Leavis, H., Top, J., Shankar, N., Borgen, K., Bonten, M., van Embden, J. & Willems, R. J. ( 2004; ). A novel putative enterococcal pathogenicity island linked to the esp virulence gene of Enterococcus faecium and associated with epidemicity. J Bacteriol 186, 672–682.[CrossRef]
    [Google Scholar]
  14. Leendertse, M., Heikens, E., Wijnands, L. M., van Luit-Asbroek, M., Teske, G. J., Roelofs, J. J., Bonten, M. J., van der Poll, T. & Willems, R. J. ( 2009; ). Enterococcal surface protein transiently aggravates Enterococcus faecium-induced urinary tract infection in mice. J Infect Dis 200, 1162–1165.[CrossRef]
    [Google Scholar]
  15. Pultz, N. J., Shankar, N., Baghdayan, A. S. & Donskey, C. J. ( 2005; ). Enterococcal surface protein Esp does not facilitate intestinal colonization or translocation of Enterococcus faecalis in clindamycin-treated mice. FEMS Microbiol Lett 242, 217–219.[CrossRef]
    [Google Scholar]
  16. Shankar, N., Lockatell, C. V., Baghdayan, A. S., Drachenberg, C., Gilmore, M. S. & Johnson, D. E. ( 2001; ). Role of Enterococcus faecalis surface protein Esp in the pathogenesis of ascending urinary tract infection. Infect Immun 69, 4366–4372.[CrossRef]
    [Google Scholar]
  17. Shankar, N., Baghdayan, A. S. & Gilmore, M. S. ( 2002; ). Modulation of virulence within a pathogenicity island in vancomycin-resistant Enterococcus faecalis. Nature 417, 746–750.[CrossRef]
    [Google Scholar]
  18. Shankar, V., Baghdayan, A. S., Huycke, M. M., Lindahl, G. & Gilmore, M. S. ( 1999; ). Infection-derived Enterococcus faecalis strains are enriched in esp, a gene encoding a novel surface protein. Infect Immun 67, 193–200.
    [Google Scholar]
  19. Singh, K. V., Nallapareddy, S. R., Sillanpaa, J. & Murray, B. E. ( 2010; ). Importance of the collagen adhesin Ace in pathogenesis and protection against Enterococcus faecalis experimental endocarditis. PLoS Pathog 6, e1000716 [CrossRef]
    [Google Scholar]
  20. Stalhammar-Carlemalm, M., Stenberg, L. & Lindahl, G. ( 1993; ). Protein rib: a novel group B streptococcal cell surface protein that confers protective immunity and is expressed by most strains causing invasive infections. J Exp Med 177, 1593–1603.[CrossRef]
    [Google Scholar]
  21. Tendolkar, P. M., Baghdayan, A. S., Gilmore, M. S. & Shankar, N. ( 2004; ). Enterococcal surface protein, Esp, enhances biofilm formation by Enterococcus faecalis. Infect Immun 72, 6032–6039.[CrossRef]
    [Google Scholar]
  22. Tendolkar, P. M., Baghdayan, A. S. & Shankar, N. ( 2005; ). The N-terminal domain of enterococcal surface protein, Esp, is sufficient for Esp-mediated biofilm enhancement in Enterococcus faecalis. J Bacteriol 187, 6213–6222.[CrossRef]
    [Google Scholar]
  23. Teng, F., Nannini, E. C. & Murray, B. E. ( 2005; ). Importance of gls24 in virulence and stress response of Enterococcus faecalis and use of the Gls24 protein as a possible immunotherapy target. J Infect Dis 191, 472–480.[CrossRef]
    [Google Scholar]
  24. Theilacker, C., Kaczynski, Z., Kropec, A., Fabretti, F., Sange, T., Holst, O. & Huebner, J. ( 2006; ). Opsonic antibodies to Enterococcus faecalis strain 12030 are directed against lipoteichoic acid. Infect Immun 74, 5703–5712.[CrossRef]
    [Google Scholar]
  25. Toledo-Arana, A., Valle, J., Solano, C., Arrizubieta, M. J., Cucarella, C., Lamata, M., Amorena, B., Leiva, J., Penades, J. R. & other authors ( 2001; ). The enterococcal surface protein, Esp, is involved in Enterococcus faecalis biofilm formation. Appl Environ Microbiol 67, 4538–4545.[CrossRef]
    [Google Scholar]
  26. Top, J., Willems, R. & Bonten, M. ( 2008; ). Emergence of CC17 Enterococcus faecium: from commensal to hospital-adapted pathogen. FEMS Immunol Med Microbiol 52, 297–308.
    [Google Scholar]
  27. van den Bogaard, A. E., Mertens, P., London, N. H. & Stobberingh, E. E. ( 1997; ). High prevalence of colonization with vancomycin- and pristinamycin-resistant enterococci in healthy humans and pigs in The Netherlands: is the addition of antibiotics to animal feeds to blame? J Antimicrob Chemother 40, 454–456.[CrossRef]
    [Google Scholar]
  28. Van Wamel, W. J., Hendrickx, A. P., Bonten, M. J., Top, J., Posthuma, G. & Willems, R. J. ( 2007; ). Growth condition-dependent Esp expression by Enterococcus faecium affects initial adherence and biofilm formation. Infect Immun 75, 924–931.[CrossRef]
    [Google Scholar]
  29. Werner, G., Coque, T. M., Hammerum, A. M., Hope, R., Hryniewicz, W., Johnson, A., Klare, I., Kristinsson, K. G., Leclercq, R. & other authors ( 2008; ). Emergence and spread of vancomycin resistance among enterococci in Europe. Euro Surveill 13, pii=19046
    [Google Scholar]
  30. Willems, R. J., Homan, W., Top, J., van Santen-Verheuvel, M., Tribe, D., Manzioros, X., Gaillard, C., Vandenbroucke-Grauls, C. M., Mascini, E. M. & other authors ( 2001; ). Variant esp gene as a marker of a distinct genetic lineage of vancomycin-resistant Enterococcus faecium spreading in hospitals. Lancet 357, 853–855.[CrossRef]
    [Google Scholar]
  31. Willems, R. J., Top, J., van Santen, M., Robinson, D. A., Coque, T. M., Baquero, F., Grundmann, H. & Bonten, M. J. ( 2005; ). Global spread of vancomycin-resistant Enterococcus faecium from distinct nosocomial genetic complex. Emerg Infect Dis 11, 821–828.[CrossRef]
    [Google Scholar]
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