@article{mbs:/content/journal/jmm/10.1099/jmm.0.020339-0, author = "Cardoso, Karen and Gandra, Rinaldo Ferreira and Wisniewski, Edirlene Sara and Osaku, Clarice Aoki and Kadowaki, Marina Kimiko and Felipach-Neto, Vicente and Haus, Leandro Fávero Aby-Ázar and Simão, Rita de Cássia Garcia", title = "DnaK and GroEL are induced in response to antibiotic and heat shock in Acinetobacter baumannii", journal= "Journal of Medical Microbiology", year = "2010", volume = "59", number = "9", pages = "1061-1068", doi = "https://doi.org/10.1099/jmm.0.020339-0", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.020339-0", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", keywords = "HSP, heat-shock protein", abstract = "We studied the expression of DnaK and GroEL in Acinetobacter baumannii cells (strains ATCC 19606 and RS4) under stress caused by heat shock or antibiotics. A Western blot assay showed that DnaK and GroEL levels increased transiently more than 2-fold after exposure of bacterial cells to heat shock for 20 min at 50 °C. Heat induction of DnaK and GroEL was blocked completely when an inhibitor of transcription, rifampicin, was added 1 min before a temperature upshift to 50 °C, suggesting that the induction of these chaperones depends on transcription. A. baumannii cells pretreated at 45 °C for 30 min were better able to survive at 50 °C for 60 min than cells pretreated at 37 °C, indicating that A. baumannii is able to acquire thermotolerance. DnaK and GroEL were successfully induced in cells pre-incubated with a subinhibitory concentration of streptomycin. Moreover, bacterial cells pretreated for 30 min at 45 °C were better able to survive streptomycin exposure than cells pretreated at physiological temperatures. DnaK expression was upregulated in a multidrug-resistant strain of A. baumannii (RS4) in the presence of different antimicrobials (ampicillin+sulbactam, cefepime, meropenem and sulphamethoxazole+trimethoprim). This study is to the best of our knowledge the first to show that A. baumannii DnaK and GroEL could play an important role in the stress response induced by antibiotics.", }