1887

Abstract

Herein we describe a real-time quantitative PCR assay for evaluating the adherence of to differentiated human intestinal Caco-2 cells. Our investigations demonstrated that the method, employing the -specific triose-phosphate isomerase gene, is as reliable but less time-consuming than counting c.f.u. We conclude that the method will be useful for evaluating the role of host cell adherence in the pathogenesis of infection.

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2010-08-01
2019-11-19
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