1887

Abstract

In this study the role of the gene in fungal drug susceptibility was investigated by disrupting and overexpressing the gene in . MIC determination and a spot assay showed that a Δ/Δ null mutant (strain T2bc) was more resistant to the antifungals tested than the wild-type (strain CAI4). Real-time RT-PCR and rhodamine 6G efflux examination showed that 2 did not influence the activity of drug efflux pumps. Sterol analysis with GC/high-resolution MS indicated that the intracellular ergosterol composition of the Δ/Δ mutant was significantly increased. Subsequently, fluorescence polarization measurements also revealed that Top2-deprived cells displayed a decrease in membrane fluidity, resulting in enhanced passive diffusion of the drugs. Quantitative real-time RT-PCR analysis further confirmed that the gene, an essential gene in ergosterol biosynthesis, was upregulated. These results demonstrate a close relationship between the gene and drug susceptibility in .

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2010-07-01
2020-07-10
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