The virulence factor genotypes of a large cohort of clinical, nosocomial environment and community environment isolates (184 in total) of Pseudomonas aeruginosa from Tasmania, Australia, were determined by PCR. The virulence factor genotype of the majority of isolates was highly conserved, with the exception of the virulence gene exoU, which demonstrated low prevalence (33 isolates; 18 %) in the population tested. Isolates collected from the environment of intensive therapy wards (intensive care unit and neurosurgical units) of the major tertiary referral hospital in Tasmania were found to be more likely (P<0.001 and P<0.05, respectively) to possess the virulence factor gene exoU than all other isolates. Adult cystic fibrosis isolates showed a decreased prevalence of the exoU gene (P<0.01) when compared to other clinical isolates (P<0.01), which may indicate decreased virulence. No specific virulence factor genotype was associated with the cystic fibrosis epidemic strains tested.
EngelJ. N.2003; Molecular pathogenesis of acute Pseudomonas aeruginosa infections. In Severe Infections Caused by Pseudomonas aeruginosa pp 201–229 Edited by
HauserA. R.,
RelloJ.
Dordrecht: Kluwer Academic Publishers;
FeltmanH.,
SchulertG.,
KhanS.,
JainM.,
PetersonL.,
HauserA. R.2001; Prevalence of type III secretion genes in clinical and environmental isolates of Pseudomonas aeruginosa
. Microbiology 147:2659–2669
JainM.,
RamirezD.,
SeshadriR.,
CullinaJ. F.,
PowersC. A.,
SchulertG. S.,
Bar-MeirM.,
SullivanC. L.,
McColleyS. A.,
HauserA. R.2004; Type III secretion phenotypes of Pseudomonas aeruginosa strains change during infection of individuals with cystic fibrosis. J Clin Microbiol 42:5229–5237[CrossRef]
KulasekaraB. R.,
KulasekaraH. D.,
WolfgangM. C.,
StevensL.,
FrankD. W.,
LoryS.2006; Acquisition and evolution of the exoU locus in Pseudomonas aeruginosa
. J Bacteriol 188:4037–4050[CrossRef]
LanotteP.,
WattS.,
MereghettiL.,
DartiguelongueL.,
Rastegar-LariA.,
GoudeauA.,
QuentinR.2004; Genetic features of Pseudomonas aeruginosa isolates from cystic fibrosis patients compared with those of isolates from other origins. J Med Microbiol 53:73–81[CrossRef]
LeeV. T.,
SmithR. S.,
TümmlerB.,
LoryS.2005; Activities of Pseudomonas aeruginosa effectors secreted by the type III secretion system in vitro and during infection. Infect Immun 73:1695–1705[CrossRef]
LinH. H.,
HuangS. P.,
TengH. C.,
JiD. D.,
ChenY. S.,
ChenY. L.2006; Presence of exoU gene of Pseudomonas aeruginosa is correlated with cytotoxicity in MDCK cells but not with colonisation in BALB/c mice. J Clin Microbiol 44:4596–4597[CrossRef]
LomholtJ. A.,
PoulsonK.,
KilianM.2001; Epidemic population structure of Pseudomonas aeruginosa : evidence for a clone that is pathogenic to the eye and that has a distinct combination of virulence factors. Infect Immun 69:6284–6295[CrossRef]
MavrodiD. V.,
BonsallR. F.,
DelaneyS. M.,
SouleM. J.,
PhillipsG.,
ThomashowL. S.2001; Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboximide from Pseudomonas aeruginosa PA01. J Bacteriol 183:6454–6466[CrossRef]
MiyataS.,
CaseyM.,
FrankD. W.,
AusubelF. M.,
DrenkardE.2003; Use of Galleria mellonella caterpillar as a model host to study the role of the type III secretion system in Pseudomonas aeruginosa pathogenesis. Infect Immun 71:2404–2413[CrossRef]
Roy-BurmanA.,
SavelR. H.,
RacineS.,
SwansonB. L.,
RevadigarN. S.,
FujimotoJ.,
SawaT.,
FrankD. W.,
Wiener-KronishJ. P.2001; Type III protein secretion is associated with death in lower respiratory and systemic Pseudomonas aeruginosa infections. J Infect Dis 183:1767–1774[CrossRef]
SchulertG. S.,
FeltmanH.,
RabinS. D.,
MartinG. C.,
BattleS. E.,
RelloJ.,
HauserA. R.2003; Secretion of the toxin ExoU is a marker for highly virulent Pseudomonas aeruginosa isolates obtained from patients with hospital-acquired pneumonia. J Infect Dis 188:1695–1706[CrossRef]
ShafikhaniS. H.,
MoralesC.,
EngelJ.2008; The Pseudomonas aeruginosa type III secreted toxin ExoT is necessary and sufficient to induce apoptosis in epithelial cells. Cell Microbiol 10:994–1007[CrossRef]
ShaverC. M.,
HauserA. R.2004; Relative contributions of Pseudomonas aeruginosa ExoU, ExoS and ExoT to virulence in the lung. Infect Immun 72:6969–6977[CrossRef]
SpilkerT.,
CoenyeT.,
VandammeP.,
LiPumaJ. J.2004; PCR-based assay for differentiation of Pseudomonas aeruginosa from other Pseudomonas species recovered from cystic fibrosis patients. J Clin Microbiol 42:2074–2079[CrossRef]
VanceR. E.,
RietschA.,
MekalanosJ. J.2005; Role of the type III secreted exoenzymes S, T and Y in systemic spread of Pseudomonas aeruginosa PA01 in vivo
. Infect Immun 73:1706–1713[CrossRef]
WarehamD. W.,
CurtisM. A.2007; A genotypic and phenotypic comparison of type III secretion profiles of Pseudomonas aeruginosa cystic fibrosis and bacteraemia isolates. Int J Med Microbiol 297:227–234[CrossRef]
Wong-BeringerR.,
Wiener-KronishJ.,
LynchS.,
FlanaganJ.2008; Comparison of type III secretion system virulence among fluoroquinolone-susceptible and -resistant clinical isolates of Pseudomonas aeruginosa
. Clin Microbiol Infect 14:330–336[CrossRef]