1887

Abstract

Macrolide resistance in 169 [group B streptococcus (GBS)] isolates originating from pregnant carriers was investigated. Using multiplex PCR the presence of genes encoding erythromycin resistance and capsular polysaccharides, as well as surface proteins, was determined. Random amplification of polymorphic DNA (RAPD) and PFGE were used to characterize specific clones among the isolates. In the examined population of women, erythromycin-resistant strains were found in 4.5 % of patients, whereas clindamycin-resistant strains were found in 3 % of patients, which was 16 % of strains resistant to erythromycin and 10 % of strains resistant to clindamycin among GBS isolates, respectively. Among the isolates, the largest percentage was represented by the constitutive macrolide–lincosamide–streptogramin B (cMLS) phenotype (63 %), then the inductive macrolide–lincosamide–streptogramin B (iMLS) phenotype (26 %) and the macrolide resistance (M) phenotype (11 %). The gene was indicated in all isolates with the cMLS phenotype and V serotype, whereas genes were found in isolates with the M phenotype and Ia serotype. Among resistance isolates, serotype V was predominant (67 %), followed by serotypes II (15 %), Ia (11 %) and III (7 %). The most common surface protein encoding genes were (70 %), then (11 %), (7.5 %), (7.5 %) and (4 %). A statistically significant relationship between macrolide resistance, serotype V and the gene was demonstrated. PFGE, in comparison to the RAPD method, gave better genetic discrimination of GBS isolates. A relatively high genetic diversity among investigated strains was shown. In addition, the largest genetic homogeneity was found in serotype V.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.018176-0
2010-07-01
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/jmm/59/7/780.html?itemId=/content/journal/jmm/10.1099/jmm.0.018176-0&mimeType=html&fmt=ahah

References

  1. Acikgoz, Z. C., Almayanlar, E., Gamberzade, S. & Gocer, S. ( 2004; ). Macrolide resistance determinants of invasive and noninvasive group B streptococci in a Turkish hospital. Antimicrob Agents Chemother 48, 1410–1412.[CrossRef]
    [Google Scholar]
  2. Brzychczy-Włoch, M., Strus, M., Pawlik, D., Gosiewski, T., Rytlewski, K., Drzewiecki, A., Lauterbach, R. & Heczko, P. B. ( 2008a; ). Studies on the possible application of molecular methods in diagnosing carriers and in similarity analysis of group B streptococci. Med Dosw Mikrobiol 60, 91–99.
    [Google Scholar]
  3. Brzychczy-Włoch, M., Strus, M., Pawlik, D., Machlarz, H., Gosiewski, T., Drzewiecki, A., Rytlewski, K., Lauterbach, R. & Heczko, P. B. ( 2008b; ). Increasing Streptococcus agalactiae colonization of pregnant women and newborns in south-eastern region of Poland. Med Dosw Mikrobiol 60, 5–12.
    [Google Scholar]
  4. Chohan, L., Hollier, L. M., Bishop, K. & Kilpatrick, C. C. ( 2006; ). Patterns of antibiotic resistance among group B Streptococcus isolates: 2001–2004. Infect Dis Obstet Gynecol 2006, 57492
    [Google Scholar]
  5. CLSI ( 2009; ). Performance Standards for Antimicrobial Disk Susceptibility Tests, approved standard, 10th edn, M02–A10, vol. 29, no. 1. Wayne, PA: Clinical and Laboratory Standards Insititute.
  6. Creti, R., Fabretti, F., Orefici, G. & Von Hunolstein, C. ( 2004; ). Multiplex PCR assay for direct identification of group B streptococcal alpha-protein-like protein genes. J Clin Microbiol 42, 1326–1329.[CrossRef]
    [Google Scholar]
  7. De Mouy, D., Cavallo, J.-D., Leclercq, R. & Fabre, R., the AFORCOPI-BIO Network ( 2001; ). Antibiotic susceptibility and mechanisms of erythromycin resistance in clinical isolates of Streptococcus agalactiae: French multicenter study. Antimicrob Agents Chemother 45, 2400–2402.[CrossRef]
    [Google Scholar]
  8. Diekema, D. J., Andrews, J. I., Huynh, H., Rhomberg, P. R., Doktor, S. R., Beyer, J., Shortride, V. D., Flamm, R. K., Jones, R. N. & other authors ( 2003; ). Molecular epidemiology of macrolide resistance in neonatal bloodstream isolates of group B streptococci. J Clin Microbiol 41, 2659–2661.[CrossRef]
    [Google Scholar]
  9. Domelier, A. S., van der Mee-Marquet, N., Arnault, L., Mereghetti, L., Lanotte, P., Rosenau, A., Lartigue, M. F. & Quentin, R. ( 2008; ). Molecular characterization of erythromycin-resistant Streptococcus agalactiae strains. J Antimicrob Chemother 62, 1227–1233.[CrossRef]
    [Google Scholar]
  10. Gherardi, G., Imperi, M., Baldassarri, L., Pataracchia, M., Alfarone, G., Recchia, S., Orefici, G., Dicuonzo, G. & Creti, R. ( 2007; ). Molecular epidemiology and distribution of serotypes, surface proteins, and antibiotic resistance among group B streptococci in Italy. J Clin Microbiol 45, 2909–2916.[CrossRef]
    [Google Scholar]
  11. Heczko, P. B., Niemiec, T., Lauterbach, R., Przondo-Mordarska, H., Strus, M., Drzewiecki, A. & Brzychczy-Włoch, M. ( 2008; ). Recommendations for the detection of group B streptococcus (GBS) carriage in pregnant women and for prevention of neonatal infections caused by this pathogen. Zakazenia 2, 87–96.
    [Google Scholar]
  12. Hsueh, P. R., Teng, L. J., Lee, L. N., Ho, S. W., Yang, P. C. & Luh, K. T. ( 2001; ). High incidence of erythromycin resistance among clinical isolates of Streptococcus agalactiae in Taiwan. Antimicrob Agents Chemother 45, 3205–3208.[CrossRef]
    [Google Scholar]
  13. Martinez, G., Harel, J., Higgins, R., Lacouture, S., Daignault, D. & Gottschalk, M. ( 2000; ). Characterization of Streptococcus agalactiae isolates of bovine and human origin by randomly amplified polymorphic DNA analysis. J Clin Microbiol 38, 71–78.
    [Google Scholar]
  14. Persson, E., Berg, S., Trollfors, B., Larsson, P., Ek, E., Backhaus, E., Claesson, B. E., Jonsson, L., Rådberg, G. & other authors ( 2004; ). Serotypes and clinical manifestations of invasive group B streptococcal infections in western Sweden 1998–2001. Clin Microbiol Infect 10, 791–796.[CrossRef]
    [Google Scholar]
  15. Persson, E., Berg, S., Bevanger, L., Bergh, K., Valso-Lyng, R. & Trollfors, B. ( 2008; ). Characterization of invasive group B streptococci based on investigation of surface proteins and genes encoding surface proteins. Clin Microbiol Infect 14, 66–73.[CrossRef]
    [Google Scholar]
  16. Poyart, C., Tazi, A., Réglier-Poupet, H., Billoet, A., Tavares, N., Raymond, J. & Trieu-Cuot, P. ( 2007; ). Multiplex PCR assay for rapid and accurate capsular typing of group B streptococci. J Clin Microbiol 45, 1985–1988.[CrossRef]
    [Google Scholar]
  17. Schoening, T. E., Wagner, J. & Arvand, M. ( 2005; ). Prevalence of erythromycin and clindamycin resistance among Streptococcus agalactiae isolates in Germany. Clin Microbiol Infect 11, 579–582.[CrossRef]
    [Google Scholar]
  18. Schrag, S., Gorwitz, R., Fultz-Butts, K. & Schuchat, A. ( 2002; ). Prevention of perinatal group B streptococcal disease. Revised guidelines from CDC. MMWR Recomm Rep 51, 1–22.
    [Google Scholar]
  19. Skjaervold, N. K., Bergh, K. & Bevanger, L. ( 2004; ). Distribution of PFGE types of invasive Norwegian group B streptococci in relation to serotypes. Indian J Med Res 119, 201–204.
    [Google Scholar]
  20. Slotved, H. C., Kong, F., Lambertsen, L., Sauer, S. & Gilbert, G. L. ( 2007; ). Serotype IX a proposed new Streptococcus agalactiae serotype. J Clin Microbiol 45, 2929–2936.[CrossRef]
    [Google Scholar]
  21. Strus, M., Pawlik, D., Brzychczy-Włoch, M., Gosiewski, T., Rytlewski, K., Lauterbach, R. & Heczko, P. B. ( 2009; ). Group B streptococcus colonization of pregnant women and their children observed on obstetric and neonatal wards of the University Hospital in Krakow, Poland. J Med Microbiol 58, 228–233.[CrossRef]
    [Google Scholar]
  22. Sutcliffe, J., Grebe, T., Tait-Kamradt, A. & Wondrack, L. ( 1996; ). Detection of erythromycin-resistant determinants by PCR. Antimicrob Agents Chemother 40, 2562–2566.
    [Google Scholar]
  23. Tynkkynen, S., Satokari, R., Saarela, M., Mattila-Sandholm, T. & Saxelin, M. ( 1999; ). Comparison of ribotyping, randomly amplified polymorphic DNA analysis, and pulsed-field gel electrophoresis in typing of Lactobacillus rhamnosus and Lactobacillus casei strains. Appl Environ Microbiol 65, 3908–3914.
    [Google Scholar]
  24. Uh, Y., Kim, H. Y., Jang, I. H., Hwang, G. Y. & Yoon, K. J. ( 2005; ). Correlation of serotypes and genotypes of macrolide-resistant Streptococcus agalactiae. Yonsei Med J 46, 480–483.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.018176-0
Loading
/content/journal/jmm/10.1099/jmm.0.018176-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error