1887

Abstract

Atypical O1 strains – hybrid strains (strains that cannot be classified either as El Tor or classical biotype) and altered strains (El Tor biotype strains that produce classical cholera toxin) – are currently prevalent in Asia and Africa. A total of 74 hybrid and altered strains that harboured classical cholera toxin were investigated by multilocus variable-number tandem repeat analysis (MLVA). The results showed that the hybrid/altered strains could be categorized into three groups and that they were distant from the El Tor strain responsible for the seventh cholera pandemic. Hybrid/altered strains with a tandem repeat of the classical CTX prophage on the small chromosome were divided into two MLVA groups (group I: Mozambique/Bangladesh group; group III: Vietnam group), and altered strains with the RS1–CTX prophage containing the El Tor type and classical on the large chromosome were placed in two MLVA groups (group II: India/Bangladesh group; group III: India/Vietnam group).

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2010-07-01
2024-03-28
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References

  1. Chatterjee S., Patra T., Ghosh K., Raychoudhuri A., Pazhani G. P., Das M., Sarkar B., Bhadra R. K., Mukhopadhyay A. K. other authors 2009; Vibrio cholerae O1 clinical strains isolated in 1992 in Kolkata with progenitor traits of the 2004 Mozambique variant. J Med Microbiol 58:239–247 [CrossRef]
    [Google Scholar]
  2. Danin-Poleg Y., Cohen L. A., Gancz H., Broza Y. Y., Goldshmidt H., Malul E., Valinsky L., Lerner L., Broza M. other authors 2007; Vibrio cholerae strain typing and phylogeny study based on simple sequence repeats. J Clin Microbiol 45:736–746 [CrossRef]
    [Google Scholar]
  3. Faruque S. M., Tam V. C., Chowdhury N., Diraphat P., Dziejman M., Heidelberg J. F., Clemens J. D., Mekalanos J. J., Nair G. B. 2007; Genomic analysis of the Mozambique strain of Vibrio cholerae O1 reveals the origin of El Tor strains carrying classical CTX prophage. Proc Natl Acad Sci U S A 104:5151–5156 [CrossRef]
    [Google Scholar]
  4. Ghosh R., Nair G. B., Tang L., Morris J. G., Sharma N. C., Ballal M., Garg P., Ramamurthy T., Stine O. C. 2008; Epidemiological study of Vibrio cholerae using variable number of tandem repeats. FEMS Microbiol Lett 288:196–201 [CrossRef]
    [Google Scholar]
  5. Goel A. K., Jain M., Kumar P., Bhadauria S., Kmboj D. V., Singh L. 2008; A new variant of Vibrio cholerae O1 El Tor causing cholera in India. J Infect 57:280–281 [CrossRef]
    [Google Scholar]
  6. Iwanaga M., Yamamoto K., Higa N., Ichinose Y., Nakasone N., Tanabe M. 1986; Culture conditions for stimulating cholera toxin production by Vibrio cholerae O1 El Tor. Microbiol Immunol 30:1075–1083 [CrossRef]
    [Google Scholar]
  7. Jeon Y. S., Chung H., Park S., Hur I., Lee J. H., Chun J. 2005; jPHYDIT: a JAVA-based integrated environment for molecular phylogeny of ribosomal RNA sequences. Bioinformatics 21:3171–3173 [CrossRef]
    [Google Scholar]
  8. Kaper J. B., Morris J. G. Jr, Levine M. M. 1995; Cholera. Clin Microbiol Rev 8:48–86
    [Google Scholar]
  9. Lee J. H., Han K. H., Choi S. Y., Lucas M. E., Mondlane C., Ansaruzzaman M., Nair G. B., Sack D. A., von Seidlein L. other authors 2006; Multilocus sequence typing (MLST) analysis of Vibrio cholerae O1 El Tor isolates from Mozambique that harbour the classical CTX prophage. J Med Microbiol 55:165–170 [CrossRef]
    [Google Scholar]
  10. Lee J. H., Choi S. Y., Jeon Y. S., Lee H. R., Kim E. J., Nguyen B. M., Hien N. T., Ansaruzzaman M., Islam M. S. other authors 2009; Classification of hybrid and altered Vibrio cholerae strains by CTX prophage and RS1 element structure. J Microbiol 47:783–788 [CrossRef]
    [Google Scholar]
  11. Nair G. B., Faruque S. M., Bhuiyan N. A., Kamruzzaman M., Siddique A. K., Sack D. A. 2002; New variants of Vibrio cholerae O1 biotype El Tor with attributes of the classical biotype from hospitalized patients with acute diarrhea in Bangladesh. J Clin Microbiol 40:3296–3299 [CrossRef]
    [Google Scholar]
  12. Nair G. B., Qadri F., Holmgren J., Svennerholm A. M., Safa A., Bhuiyan N. A., Ahmad Q. S., Faruque S. M., Faruque A. S. other authors 2006; Cholera due to altered El Tor strains of Vibrio cholerae O1 in Bangladesh. J Clin Microbiol 44:4211–4213 [CrossRef]
    [Google Scholar]
  13. Nguyen B. M., Lee J. H., Cuong N. T., Choi S. Y., Hien N. T., Anh D. D., Lee H. R., Ansaruzzaman M., Endtz H. P. other authors 2009; Cholera outbreaks caused by an altered Vibrio cholerae O1 El Tor biotype strain producing classical cholera toxin B in Vietnam in 2007 to 2008. J Clin Microbiol 47:1568–1571 [CrossRef]
    [Google Scholar]
  14. Raychoudhuri A., Mukhopadhyay A. K., Ramamurthy T., Nandy R. K., Takeda Y., Nair G. B. 2008; Biotyping of Vibrio cholerae O1: time to redefine the scheme. Indian J Med Res 128:695–698
    [Google Scholar]
  15. Roychowdhury A., Pan A., Dutta D., Mukhopadhyay A. K., Ramamurthy T., Nandy R. K., Bhattacharya S. K., Bhattacharya M. K. 2008; Emergence of tetracycline-resistant Vibrio cholerae O1 serotype Inaba, in Kolkata, India. Jpn J Infect Dis 61:128–129
    [Google Scholar]
  16. Sack D. A., Sack R. B., Nair G. B., Siddique A. K. 2004; Cholera. Lancet 363:223–233 [CrossRef]
    [Google Scholar]
  17. Safa A., Bhuyian N. A., Nusrin S., Ansaruzzaman M., Alam M., Hamabata T., Takeda Y., Sack D. A., Nair G. B. 2006; Genetic characteristics of Matlab variants of Vibrio cholerae O1 that are hybrids between classical and El Tor biotypes. J Med Microbiol 55:1563–1569 [CrossRef]
    [Google Scholar]
  18. Safa A., Nair G. B., Kong R. Y. 2010; Evolution of new variants of Vibrio cholerae O1. Trends Microbiol 18:46–54 [CrossRef]
    [Google Scholar]
  19. Stine O. C., Alam M., Tang L., Nair G. B., Siddique A. K., Faruque S. M., Huq A., Colwell R., Sack R. B. other authors 2008; Seasonal cholera from multiple small outbreaks, rural Bangladesh. Emerg Infect Dis 14:831–833 [CrossRef]
    [Google Scholar]
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