1887

Abstract

, the aetiological agent of pneumonia and non-gonococcal urethritis, shares a high degree of DNA sequence identity with the viridans group of streptococci, particularly and . Although their clinical and pathological manifestations are different, discrimination between and its close viridans cocci relatives is still quite difficult. Suppression subtractive hybridization was performed to identify the genomic differences between and . Thirty-four resulting -specific clones were examined by sequence determination and comparative DNA sequence analysis using . -specific primers were subsequently designed from one of the clonal DNA sequences containing the gene (coding for capsular polysaccharide biosynthesis). The primer specificities were evaluated using 49 viridans streptococci including 26 , 54 other streptococci, 14 species, 14 species and three species, and compared with the specificities of previously described autolysin (), pneumolysin (), Spn9802 and Spn9828 primers. The newly designed -specific primer set was highly specific to and was even better than the existing primers. These findings may help improve the rapid identification and differentiation of from closely related members of the viridans group streptococci.

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2010-10-01
2019-10-22
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