1887

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Volume 59, Issue 6

Characterization of isolates from patients in Japan according to serogroups, monoclonal antibody subgroups and sequence types Free

Abstract

We collected 86 unrelated clinical strains that were isolated in Japan during the period 1980–2008. Most (80.2 %) belonged to serogroup 1, followed by serogroups 5, 3 and 2. Interestingly, the patients with serogroup 1 had a significantly higher male-to-female ratio (12.4) than the patients with other serogroups (2.0) (OR, 10.5; 95 % CI, 2.5–44.5). When the serogroup 1 strains were analysed by monoclonal antibody (mAb) typing, the most prevalent subgroup was Benidorm (34.9 % of all isolates). Moreover, 79.7 % of the serogroup 1 isolates were bound by mAb 3/1, which recognizes the virulence-associated epitope. When all 86 isolates were subjected to sequence-based typing (SBT) using seven loci, they could be divided into 53 sequence types (STs). The ST with the most isolates (seven) was ST1, to which most isolates from patients and environments around the world belong. However, six of the seven ST1 isolates were isolated before 1994. Other major STs were ST306 (=6), ST120 (=5) and ST138 (=5). All ST306 and ST138 isolates, except for one isolate (ST306), were suspected or confirmed to be derived from bath water, which suggests that these strains prefer bath habitats. The sources of all ST1 and ST120 isolates remain unclear. By combining the SBT and mAb data, the 86 isolates could be divided into 59 types (discrimination index, 0.984). This confirms the usefulness of this combination in epidemiological studies.

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Keyword(s): EWGLI, European Working Group on Legionella Infections , SBT, sequence-based typing and ST, sequence type
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2010-06-01
2024-04-25
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References

  1. Amemura-Maekawa J., Kura F., Chang B., Watanabe H. 2005; Legionella pneumophila serogroup 1 isolates from cooling towers in Japan form a distinct genetic cluster. Microbiol Immunol 49:1027–1033 [CrossRef]
     [Google Scholar]
  2. Amemura-Maekawa J., Kura F., Chang B., Suzuki-Hashimoto A., Ichinose M., Endo T., Watanabe H. 2008; Distinct difference of flaA genotypes of Legionella pneumophila between isolates from bath water and cooling tower water. Microbiol Immunol 52:460–464 [CrossRef]
     [Google Scholar]
  3. Borchardt J., Helbig J. H., Lück P. C. 2008; Occurrence and distribution of sequence types among Legionella pneumophila strains isolated from patients in Germany: common features and differences to other regions of the world. Eur J Clin Microbiol Infect Dis 27:29–36
     [Google Scholar]
  4. Brenner D. J., Steigerwalt A. G., Epple P., Bibb W. F., McKinney R. M., Starnes R. W., Colville J. M., Selander R. K., Edelstein P. H., Moss C. W. 1988; Legionella pneumophila serogroup Lansing 3 isolated from a patient with fatal pneumonia, and descriptions of L.pneumophila subsp. pneumophila subsp. nov., L. pneumophila subsp. fraseri subsp. nov., and L. pneumophila subsp. pascullei subsp. nov. J Clin Microbiol 26:1695–1703
     [Google Scholar]
  5. Cazalet C., Jarraud S., Ghavi-Helm Y., Kunst F., Glaser P., Etienne J., Buchrieser C. 2008; Multigenome analysis identifies a worldwide distributed epidemic Legionella pneumophila clone that emerged within a highly diverse species. Genome Res 18:431–441 [CrossRef]
     [Google Scholar]
  6. Fisman D. N., Lim S., Wellenius G. A., Johnson C., Britz P., Gaskins M., Maher J., Mittleman M. A., Spain C. V. other authors 2005; It's not the heat, it's the humidity: wet weather increases legionellosis risk in the greater Philadelphia metropolitan area. J Infect Dis 192:2066–2073 [CrossRef]
     [Google Scholar]
  7. Gaia V., Fry N. K., Afshar B., Lück P. C., Meugnier H., Etienne J., Peduzzi R., Harrison T. G. 2005; Consensus sequence-based scheme for epidemiological typing of clinical and environmental isolates of Legionella pneumophila . J Clin Microbiol 43:2047–2052 [CrossRef]
     [Google Scholar]
  8. Harrison T. G., Afshar B., Doshi N., Fry N. K., Lee J. V. 2009; Distribution of Legionella pneumophila serogroups, monoclonal antibody subgroups and DNA sequence types in recent clinical and environmental isolates from England and Wales (2000–2008). Eur J Clin Microbiol Infect Dis 28:781–791 [CrossRef]
     [Google Scholar]
  9. Helbig J. H., Kurtz J. B., Pastoris M. C., Pelaz C., Lück P. C. 1997; Antigenic lipopolysaccharide components of Legionella pneumophila recognized by monoclonal antibodies: possibilities and limitations for division of the species into serogroups. J Clin Microbiol 35:2841–2845
     [Google Scholar]
  10. Helbig J. H., Bernander S., Castellani Pastoris M., Etienne J., Gaia V., Lauwers S., Lindsay D., Lück P. C., Marques T. other authors 2002; Pan-European study on culture-proven Legionnaires' disease: distribution of Legionella pneumophila serogroups and monoclonal subgroups. Eur J Clin Microbiol Infect Dis 21:710–716 [CrossRef]
     [Google Scholar]
  11. Hunter P. R., Gaston M. A. 1988; Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity. J Clin Microbiol 26:2465–2466
     [Google Scholar]
  12. Infectious Disease Surveillance Center 2000 Legionellosis April 1999-July 2000 IASR 21:186–187
     [Google Scholar]
  13. Infectious Disease Surveillance Center 2003; Legionellosis; April 1999-December 2002; Japan. IASR 24:27–28
     [Google Scholar]
  14. Infectious Disease Surveillance Center 2008; Legionellosis; January 2003-September 2008; Japan. IASR 29:327–328
     [Google Scholar]
  15. Isozumi R., Ito Y., Ito I., Osawa M., Hirai T., Takakura S., Iinuma Y., Ichiyama S., Tateda K. other authors 2005; An outbreak of Legionella pneumonia originating from a cooling tower. Scand J Infect Dis 37:709–711 [CrossRef]
     [Google Scholar]
  16. Kozak N. A., Benson R. F., Brown E., Alexander N. T., Taylor T. H. Jr, Shelton B. G., Fields B. S. 2009; Distribution of lag-1 alleles and sequence-based types among Legionella pneumophila serogroup 1 clinical and environmental isolates in the United States. J Clin Microbiol 47:2525–2535 [CrossRef]
     [Google Scholar]
  17. Kura F., Amemura-Maekawa J., Yagita K., Endo T., Ikeno M., Tsuji H., Taguchi M., Kobayashi K., Ishii E., Watanabe H. 2006; Outbreak of Legionnaires' disease on a cruise ship linked to spa-bath filter stones contaminated with Legionella pneumophila serogroup 5. Epidemiol Infect 134:385–391
     [Google Scholar]
  18. Kuroki H., Miyamoto H., Fukuda K., Iihara H., Kawamura Y., Ogawa M., Wang Y., Ezaki T., Taniguchi H. 2007; Legionella impletisoli sp. nov. and Legionella yabuuchiae sp. nov., isolated from soils contaminated with industrial wastes in Japan. Syst Appl Microbiol 30:273–279 [CrossRef]
     [Google Scholar]
  19. Kuroki T., Ishihara T., Ito K., Kura F. 2009; Bathwater-associated cases of legionellosis in Japan, with a special focus on Legionella concentrations in water. Jpn J Infect Dis 62:201–205
     [Google Scholar]
  20. Maesaki S., Kohno S., Koga H., Kaku M., Yoshitomi Y., Yamada H., Matsuda H., Higashiyama Y., Hara K. other authors 1992; An outbreak of Legionnaires' pneumonia in a nursing home. Intern Med 31:508–512 [CrossRef]
     [Google Scholar]
  21. Nakamura H., Yagyu H., Kishi K., Tsuchida F., Oh-Ishi S., Yamaguchi K., Matsuoka T. 2003; A large outbreak of Legionnaires' disease due to an inadequate circulating and filtration system for bath water – epidemiologic manifestations. Intern Med 42:806–811 [CrossRef]
     [Google Scholar]
  22. Neil K., Berkelman R. 2008; Increasing incidence of legionellosis in the United States, 1990–2005: changing epidemiologic trends. Clin Infect Dis 47:591–599 [CrossRef]
     [Google Scholar]
  23. Ng V., Tang P., Jamieson F., Drews S. J., Brown S., Low D. E., Johnson C. C., Fisman D. N. 2008; Going with the flow: legionellosis risk in Toronto, Canada is strongly associated with local watershed hydrology. EcoHealth 5:482–490 [CrossRef]
     [Google Scholar]
  24. Okada M., Kawano K., Kura F., Amemura-Maekawa J., Watanabe H., Yagita K., Endo T., Suzuki S. 2005; The largest outbreak of legionellosis in Japan associated with spa baths: epidemic curve and environmental investigation. Kansenshogaku Zasshi 79:365–374 (in Japanese [CrossRef]
     [Google Scholar]
  25. Ratzow S., Gaia V., Helbig J. H., Fry N. K., Lück P. C. 2007; Addition of neuA , the gene encoding N -acylneuraminate cytidylyl transferase, increases the discriminatory ability of the consensus sequence-based scheme for typing Legionella pneumophila serogroup 1 strains. J Clin Microbiol 45:1965–1968 [CrossRef]
     [Google Scholar]
  26. Reimer A. R., Au S., Schindle S., Bernard K. A. 2010; Legionella pneumophila monoclonal antibody subgroups and DNA sequence types isolated in Canada between 1981 and 2009. Laboratory Component of National. Eur J Clin Microbiol Infect Dis 29:191–205 [CrossRef]
     [Google Scholar]
  27. Ricketts K. D., Joseph C. A. European Working Group for Legionella Infections 2007; Legionnaires disease in Europe: 2005–2006. Euro Surveill 12:E7–E8
     [Google Scholar]
  28. Sakamoto R., Ohno A., Nakahara T., Satomura K., Iwanaga S., Kouyama Y., Kura F., Kato N., Matsubayashi K. & other authors (2009a). Is a puddle on asphalt roads on rainy days a risk for Legionnaires' disease?. Emerg Infect Dis 15:1295–1297 [CrossRef]
     [Google Scholar]
  29. Sakamoto R., Ohno A., Nakahara T., Satomura K., Iwanaga S., Kouyama Y., Kura F., Noami M., Kusaka K. other authors 2009b; Is driving a car a risk for Legionnaires' disease?. Epidemiol Infect 137:1615–1622 [CrossRef]
     [Google Scholar]
  30. Tijet N., Tang P., Romilowych M., Duncan C., Ng V., Fisman D. N., Jamieson F., Low D. E., Guyard C. 2010; New endemic Legionella pneumophila serogroup I clones, Ontario, Canada. Emerg Infect Dis 16:447–454 [CrossRef]
     [Google Scholar]
  31. Underwood A., Bellamy W., Afshar B., Fry N., Harrison T. 2006; Development of an online tool for the European Working Group for Legionella Infections sequence-based typing, including automatic quality assessment and data submission. In Legionella: State of the Art 30 Years After Its Recognition . pp 163–166 Edited by Cianciotto N. P., Abu Kwaik Y., Edelstein P. H., Fields B. S., Geary D. F., Harrison T. G., Joseph C. A., Ratcliff R. M., Stout J. E., Swanson M. S. Washington, DC: American Society for Microbiology;
  32. Yu V. L., Plouffe J. F., Pastoris M. C., Stout J. E., Schousboe M., Widmer A., Summersgill J., File T., Heath C. M. other authors 2002; Distribution of Legionella species and serogroups isolated by culture in patients with sporadic community-acquired legionellosis: an international collaborative survey. J Infect Dis 186:127–128 [CrossRef]
     [Google Scholar]
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Characterization of Legionella pneumophila isolates from patients in Japan according to serogroups, monoclonal antibody subgroups and sequence types
J. Med. Microbiol. 59, 653 (2010); https://doi.org/10.1099/jmm.0.017509-0
/content/journal/jmm/10.1099/jmm.0.017509-0
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