1887

Abstract

is the major cause of invasive aspergillosis (IA), a disease associated with high rates of morbidity and mortality in patients undergoing treatment for haematological malignancies. This study investigated growth and in a murine model of IA in order to provide insights into the dynamics of extracellular DNA and galactomannan (GM) release and their relevance to early diagnosis of IA. Following inoculation of whole blood with 20 conidia ml, DNA that corresponded to the inoculum could be detected by PCR but GM was not detected in plasma separated from the blood sample, indicating that the fungus did not grow in whole blood. The quantities of DNA detected by PCR, and GM, were proportional to the amount of fungal biomass present . Fungal DNA could be detected in the sera of mice experimentally infected with with maximum detection in cyclophosphamide-treated mice.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.017418-0
2010-04-01
2019-11-21
Loading full text...

Full text loading...

/deliver/fulltext/jmm/59/4/408.html?itemId=/content/journal/jmm/10.1099/jmm.0.017418-0&mimeType=html&fmt=ahah

References

  1. Alexander, B. D. ( 2002; ). Diagnosis offungal infection: new technologies for the mycology laboratory. Transpl Infect Dis 4 (Suppl.3), 32–37.[CrossRef]
    [Google Scholar]
  2. Botterel, F., Farrugia, C., Ichai, P., Costa, J. M., Saliba,F. & Bretagne, S. ( 2008; ). Real-time PCR on thefirst galactomannan-positive serum sample for diagnosing invasive aspergillosisin liver transplant recipients. Transpl Infect Dis 10, 333–338.[CrossRef]
    [Google Scholar]
  3. Bowman, J. C., Abruzzo, G. K., Anderson, J. W., Flattery, A.M., Gill, C. J., Pikounis, V. B., Schmatz, D. M., Liberator, P. A. & Douglas,C. M. ( 2001; ). Quantitative PCR assay to measure Aspergillus fumigatus burden in a murine model of disseminated aspergillosis:demonstration of efficacy of caspofungin acetate. Antimicrob AgentsChemother 45, 3474–3481.
    [Google Scholar]
  4. Cuenca-Estrella, M., Meije, Y., Diaz-Pedroche, C., Gomez-Lopez,A., Buitrago, M. J., Bernal-Martinez, L., Grande, C., Juan, R. S., Lizasoain,M. & other authors ( 2009; ). Value of serial quantificationof fungal DNA by a real-time PCR-based technique for early diagnosis of invasiveaspergillosis in patients with febrile neutropenia. J Clin Microbiol 47, 379–384.[CrossRef]
    [Google Scholar]
  5. de Pauw, B., Walsh, T. J., Donnelly, J. P., Stevens, D. A.,Edwards, J. E., Calandra, T., Pappas, P. G., Maertens, J., Lortholary, O. &other authors ( 2008; ). Revised definitions of invasivefungal disease from the European Organization for Research and Treatment ofCancer/Invasive Fungal Infections Cooperative Group and the National Instituteof Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG)Consensus Group. Clin Infect Dis 46, 1813–1821.[CrossRef]
    [Google Scholar]
  6. Gifford, A. H., Klippenstein, J. R. & Moore, M. M. ( 2002; ). Serum stimulates growth of and proteinase secretionby Aspergillus fumigatus. Infect Immun 70, 19–26.[CrossRef]
    [Google Scholar]
  7. Izano, E. A., Amarante, M. A., Kher, W. B. & Kaplan, J.B. ( 2008; ). Differential roles of poly-N-acetylglucosaminesurface polysaccharide and extracellular DNA in Staphylococcus aureus and Staphylococcus epidermidis biofilms. Appl EnvironMicrobiol 74, 470–476.
    [Google Scholar]
  8. Kasai, M., Francesconi, A., Petraitiene, R., Petraitis, V.,Kelaher, A. M., Kim, H. S., Meletiadis, J., Sein, T., Bacher, J. & Walsh,T. J. ( 2006; ). Use of quantitative real-time PCR tostudy the kinetics of extracellular DNA released from Candida albicans, with implications for diagnosis of invasive candidiasis. J Clin Microbiol 44, 143–150.[CrossRef]
    [Google Scholar]
  9. Lin, J. S., Yang, C. W., Wang, D. W. & Wu-Hsieh, B. A. ( 2005; ). Dendritic cells cross-present exogenous fungal antigensto stimulate a protective CD8 T cell response in infection by Histoplasmacapsulatum. J Immunol 174, 6282–6291.[CrossRef]
    [Google Scholar]
  10. Mengoli, C., Cruciani, M., Barnes, R. A., Loeffler, J. &Donnelly, J. P. ( 2009; ). Use of PCR for diagnosis ofinvasive aspergillosis: systematic review and meta-analysis. LancetInfect Dis 9, 89–96.
    [Google Scholar]
  11. Mennink-Kersten, M. A., Ruegebrink, D., Wasei, N., Melchers,W. J. & Verweij, P. E. ( 2006; ). In vitro releaseby Aspergillus fumigatus of galactofuranose antigens, 1,3-beta-d-glucan, and DNA, surrogate markers used for diagnosis of invasiveaspergillosis. J Clin Microbiol 44, 1711–1718.[CrossRef]
    [Google Scholar]
  12. Miyazato, A., Nakamura, K., Yamamoto, N., Mora-Montes, H. M.,Tanaka, M., Abe, Y., Tanno, D., Inden, K., Gang, X. & other authors ( 2009; ). Toll-like receptor 9-dependent activation of myeloiddendritic cells by Deoxynucleic acids from Candida albicans. Infect Immun 77, 3056–3064.[CrossRef]
    [Google Scholar]
  13. Mousavi, S. A. & Robson, G. D. ( 2003; ). Entry into the stationary phase is associated with a rapid loss of viabilityand an apoptotic-like phenotype in the opportunistic pathogen Aspergillusfumigatus. Fungal Genet Biol 39, 221–229.[CrossRef]
    [Google Scholar]
  14. Mowat, E., Williams, C., Jones, B., McChlery, S. & Ramage,G. ( 2009; ). The characteristics of Aspergillusfumigatus mycetoma development: is this a biofilm? Med Mycol 47 (Suppl. 1), S120–S126.[CrossRef]
    [Google Scholar]
  15. Neofytos, D., Horn, D., Anaissie, E., Steinbach, W., Olyaei,A., Fishman, J., Pfaller, M., Chang, C., Webster, K. & Marr, K. ( 2009; ). Epidemiology and outcome of invasive fungal infectionin adult hematopoietic stem cell transplant recipients: analysis of MulticenterProspective Antifungal Therapy (PATH) Alliance registry. Clin Infect Dis 48, 265–273.[CrossRef]
    [Google Scholar]
  16. Perlin, D. S. & Zhao, Y. ( 2009; ).Molecular diagnostic platforms for detecting Aspergillus. Med Mycol 47 (Suppl. 1), S223–S232.[CrossRef]
    [Google Scholar]
  17. Romani, L., Fallarino, F., De Luca, A., Montagnoli, C., D'Angelo,C., Zelante, T., Vacca, C., Bistoni, F., Fioretti, M. C. & other authors ( 2008; ). Defective tryptophan catabolism underliesinflammation in mouse chronic granulomatous disease. Nature 451, 211–215.[CrossRef]
    [Google Scholar]
  18. Schmidt, B., Weickmann, S., Witt, C. & Fleischhacker, M. ( 2005; ). Improved method for isolating cell-free DNA. Clin Chem 51, 1561–1563.[CrossRef]
    [Google Scholar]
  19. Stephens-Romero, S. D., Mednick, A. J. & Feldmesser, M. ( 2005; ). The pathogenesis of fatal outcome in murinepulmonary aspergillosis depends on the neutrophil depletion strategy. Infect Immun 73, 114–125.[CrossRef]
    [Google Scholar]
  20. Suarez, F., Lortholary, O., Buland, S., Rubio, M. T., Ghez,D., Mahé, V., Quesne, G., Poirée, S., Buzyn, A. & otherauthors ( 2008; ). Detection of circulating Aspergillusfumigatus DNA by real-time PCR assay of large serum volumes improvesearly diagnosis of invasive aspergillosis in high-risk adult patients underhematologic surveillance. J Clin Microbiol 46, 3772–3777.[CrossRef]
    [Google Scholar]
  21. White, P. L. & Barnes, R. A. ( 2009; ). Aspergillus PCR. In Aspergillus fumigatus and Aspergillosis, pp. 373–388. Edited by J. Latge & W. Steinbach. Washington,DC: American Society for Microbiology.
  22. White, P. L., Perry, M. D. & Barnes, R. A. ( 2009; ). An update on the molecular diagnosis of invasive fungaldisease. FEMS Microbiol Lett 296, 1–10.[CrossRef]
    [Google Scholar]
  23. Zarember, K. A., Sugui, J. A., Chang, Y. C., Kwon-Chung, K.J. & Gallin, J. I. ( 2007; ). Human polymorphonuclearleukocytes inhibit Aspergillus fumigatus conidial growth by lactoferrin-mediatediron depletion. J Immunol 178, 6367–6373.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.017418-0
Loading
/content/journal/jmm/10.1099/jmm.0.017418-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error