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Two cases involving polymicrobial culture-negative samples were investigatedby 16S rRNA gene sequencing, with analysis of mixed chromatograms. Fusobacteriumnecrophorum, Prevotella intermedia and Streptococcus constellatus were identified from pleural fluid in a patient with Lemierre'ssyndrome and Neisseria meningitidis and Escherichia coliwere identified from a petechia in a patient with meningococcal disease.
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