1887

Abstract

Acute lung injuries due to acute lung infections remain a major cause ofmortality. Thus a combination of an antibiotic and a compound with immunomodulatoryand anti-inflammatory activities can help to overcome acute lung infection-inducedinjuries. Curcumin derived from the rhizome of turmeric has been used fordecades and it exhibits anti-inflammatory, anti-carcinogenic, immunomodulatoryproperties by downregulation of various inflammatory mediators. Keeping theseproperties in mind, we investigated the anti-inflammatory properties of curcuminin a mouse model of acute inflammation by introducing B5055 into BALB/c mice via the intranasal route. Intranasal instillationof bacteria in this mouse model of acute pneumonia-induced inflammation resultedin a significant increase in neutrophil infiltration in the lungs along withincreased production of various inflammatory mediators [i.e. malondialdehyde (MDA),myeloperoxidase (MPO), nitric oxide (NO), tumour necrosisfactor (TNF)-] in the lung tissue. The animalsthat received curcumin alone orally or in combination with augmentin, 15 daysprior to bacterial instillation into the lungs via the intranasal route, showeda significant ( <0.05) decrease in neutrophil influxinto the lungs and a significant ( <0.05) decreasein the production of MDA, NO, MPO activity and TNF- levels.Augmentin treatment alone did not decrease the MDA, MPO, NO and TNF- levels significantly ( >0.05) as compared tothe control group. We therefore conclude that curcumin ameliorates lung inflammationinduced by B5055 without significantly ( <0.05) decreasing the bacterial load in the lung tissue whereasaugmentin takes care of bacterial proliferation. Hence, curcumin can be usedas an adjunct therapy along with antibiotics as an anti-inflammatory or animmunomodulatory agent in the case of acute lung infection.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.016873-0
2010-04-01
2020-07-13
Loading full text...

Full text loading...

/deliver/fulltext/jmm/59/4/429.html?itemId=/content/journal/jmm/10.1099/jmm.0.016873-0&mimeType=html&fmt=ahah

References

  1. Aggarwal B. B., Sung B. 2008).Pharmacological; basis for the role of curcumin in chronic diseases: an age-oldspice with modern targets. Trends Pharmacol Sci 30:85–94
    [Google Scholar]
  2. Ammon H. P. T., Wahl M. A. 1991; Pharmacology of Curcuma longa . Planta Med 57:1–7 [CrossRef]
    [Google Scholar]
  3. Baron R. M., Carvajal I. M., Liu X., Okabe R. O., Fredenburgh L. E., Macias A. A., Chen Y. H., Ejima K., Layne M. D., Perrella M. A. 2004; Reduction of nitric oxide synthase 2expression by distamycin A improves survival from endotoxemia. J Immunol 173:4147–4153 [CrossRef]
    [Google Scholar]
  4. Bhaumik S., Jyothi M. D., Khar A. 2000; Differential modulation of nitric oxide production by curcuminin host macrophages and NK cells. FEBS Lett 483:78–82 [CrossRef]
    [Google Scholar]
  5. Brouet I., Ohshima H. 1995).Curcumin; an anti-tumor promoter and anti-inflammatory agent, inhibits inductionof nitric oxide synthase in activated macrophages. Biochem BiophysRes Commun 206:533–540
    [Google Scholar]
  6. Cheng A. L., Hsu C. H., Lin J. K., Hsu M. M., Ho Y. F., Shen T. S., Ko J. Y., Lin J. T., Lin B. R. other authors 2001; Phase I clinical trial of curcumin, a chemopreventiveagent, in patients with high-risk or pre-malignant lesions. AnticancerRes 21:2895–2900
    [Google Scholar]
  7. Cobb J. P., Natanson C., Hoffman W. D., Lodato R. F., Banks S., Koev C. A., Solomon M. A., Elin R. J., Hosseini J. M., Danner R. L. 1992; N -amino-l-arginine,an inhibitor of nitric oxide synthase, raises vascular resistance but increasesmortality rates in awake canines challenged with endotoxin. J ExpMed 176:1175–1182
    [Google Scholar]
  8. Cortes G., Borrell N., de Astroza B., Gomez C., Sauleda J., Alberti S. 2002; Molecular analysis ofthe contribution of the capsular polysaccharide and the lipopolysaccharideside chain to the virulence of Klebsiella pneumoniae in a murinemodel of pneumonia. Infect Immun 70:2583–2590 [CrossRef]
    [Google Scholar]
  9. Doran K. S., Chang J. C., Benoit V. M., Eckmann L, Nizet V. 2002; Group B streptococcal beta-hemolysin/cytolysinpromotes invasion of human lung epithelial cells and the release of interleukin-8. J Infect Dis 185:196–203 [CrossRef]
    [Google Scholar]
  10. Greenberger M. J., Strieter R. M., Kunkel S. L., Danforth J. M., Goodman R. E., Standiford T. J. 1995; Neutralization of IL-10 increases survival in a murine model of Klebsiella pneumoniae . J Immunol 155:722–729
    [Google Scholar]
  11. Held T. K., Mielke M. E. A., Chedid M., Unger M., Trautman M., Huhn D., Cross A. S. 1998; Granulocytecolony-stimulating factor worsens the outcome of the experimental Klebsiellapneumoniae pneumonia through direct interaction with the bacteria. Blood 91:2525–2533
    [Google Scholar]
  12. Huang M. T., Lou Y. R., Ma W., Newmark H. L., Reuhl K. R. 1994; Inhibitory effects of dietary curcuminon forestomach, duodenal and colon carcinogenesis in mice. CancerRes 54:5841–5847
    [Google Scholar]
  13. Huang M. T., Newmark H. L., Frenkel K. 1997; Inhibitory effects of curcumin on tumorigenesis in mice. J Cell Biochem Suppl 27:26–34
    [Google Scholar]
  14. Hunter P. A., Coleman H. K., Fisher J., Taylor D. 1980; In vitro synergistic properties of clavulanate withamoxicillin. J Antimicrob Chemother 6:455–470 [CrossRef]
    [Google Scholar]
  15. Kaur G, Tirkey N, Bharrhan S, Chanana V, Rishi P, Chopra K. 2006; Inhibition of oxidative stressand cytokine activity by curcumin in amelioration of endotoxin-induced experimentalhepatoxicity in rodents. Clin Exp Immunol 145:313–321 [CrossRef]
    [Google Scholar]
  16. Kawamori T., Lubet R., Steele V. E., Kelloff G. J., Kaskey R. B., Rao C. V. 1999; Chemopreventive effectof curcumin, a naturally occurring anti-inflammatory agent, during the promotion/progressionstages of colon cancer. Cancer Res 59:597–601
    [Google Scholar]
  17. Kooguchi K., Hashimoto S., Kobayashi A., Kitamura Y., Kudoh I., Wiener-Kronish J., Sawa T. 1998; Roleof alveolar macrophages in initiation and regulation of inflammation in Pseudomonas aeruginosa pneumonia. Infect Immun 66:3164–3169
    [Google Scholar]
  18. Kooy N. W., Royall J. A., Ye Y. Z., Kelly D. R., Beckman J. S. 1995; Evidence for in vivo peroxynitrite productionin human acute lung injury. Am J Respir Crit Care Med 151:1250–1254
    [Google Scholar]
  19. Kristof A. S., Goldberg P., Laubach V., Hussaiu S.N. 1998; Role of inducible nitric oxide synthasein endotoxin-induced acute lung injury. Am J Respir Crit Care Med 158:1883–1889 [CrossRef]
    [Google Scholar]
  20. Kumar V., Chhibber S. 2008).Anti-inflammatory; effect of thalidomide alone or in combination with augmentinin Klebsiella pneumoniae B5055 induced acute lung infection in BALB/cmice. Eur J Pharmacol 592:146–150 [CrossRef]
    [Google Scholar]
  21. Legnani D. 1997; Role of oral antibioticsin treatment of community-acquired lower respiratory tract infections. Diagn Microbiol Infect Dis 27:41–47 [CrossRef]
    [Google Scholar]
  22. Literat A., Su F., Norwicki M., Durand M., Ramanathan R., Jones C. A., Minoo P., Kwong K. Y. (2001).Regulation of pro-inflammatory cytokine expression by curcumin in hyalinemembrane disease (HMD). Life Sci 70:253–256 [CrossRef]
    [Google Scholar]
  23. Marcho Z., White J. E., Higgins P. J., Tsan M. F. 1991; Tumor necrosis factor enhances endothelial cell susceptibilityto oxygen toxicity: role of glutathione. Am J Respir Cell Mol Biol 5:556–562 [CrossRef]
    [Google Scholar]
  24. Maus U, Huwe J, Ermert L, Ermert M, Seeger W, Lohmeyer J. 2002; Molecular pathways of monocyteemigration into the alveolar air space of intact mice. Am J RespirCrit Care Med 165:95–98
    [Google Scholar]
  25. Maus U. A., Waelsch K., Kuzeil W. A., Delbeck T., Mack M., Blackwell T. S. 2003; Monocytes are potentfacilitators of alveolar neutrophil emigration during lung inflammation: roleof CCL2–CCR2 axis. J Immunol 170:3273–3278 [CrossRef]
    [Google Scholar]
  26. Mikamo H, Johri A. K., Paoletti L. C., Madoff L. C., Onderdonk A. B. 2004; Adherence to, invasion by,and cytokine production in response to serotype VIII group B streptococci. Infect Immun 72:4716–4722 [CrossRef]
    [Google Scholar]
  27. Minnard E. A., Shou J, Naama H, Cech A, Gallagher H, Daly J. M. 1994; Inhibition of nitric oxide synthesisis detrimental during endotoxemia. Arch Surg 129:142–148 [CrossRef]
    [Google Scholar]
  28. Murdoch C., Read R. C., Zhang Q., Finn A. 2002; Choline binding protein A of Streptococcus pneumoniae elicits chemokine production and expression of intercellular adhesionmolecule 1 (CD54) by human alveolar epithelial cells. J Infect Dis 186:1253–1260 [CrossRef]
    [Google Scholar]
  29. Nava E., Palmer R. M., Moncada S. 1991; Inhibition of nitric oxide synthesis in septic shock: how muchis beneficial?. Lancet 338:1555–1557 [CrossRef]
    [Google Scholar]
  30. Ohene-Abuakwa Y., Pignatelli M. 2000; Adhesion molecules in cancer biology. Adv Exp Med Biol 465:115–126
    [Google Scholar]
  31. Ohkawa H., Ohishi N., Yagi K. 1979; Assay of lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358 [CrossRef]
    [Google Scholar]
  32. Robson R. L., McLoughlin R. M., Witowski J., Loetschler P., Wilkinson T. S., Jones S. A., Topley N. 2001; Differential regulation of chemokine production in human peritoneal mesothelialcells: IFN- γ controls neutrophil migration across the mesotheliumin vitro and in vivo. J Immunol 167:1028–1031 [CrossRef]
    [Google Scholar]
  33. Rosseau S., Hammerl P., Maus U., Walmrath H. D., Schutte H., Grimminger F., Seeger W., Lohmeyer J. 2000; Phenotypic characterization of alveolar monocyte recruitment in acuterespiratory distress syndrome. Am J Physiol Lung Cell Mol Physiol 279:L25–L30
    [Google Scholar]
  34. Saint S., Chenoweth C. E. 2003; Biofilms and catheter-associated urinary tract infections. Infect Dis Clin North Am 17:411–432 [CrossRef]
    [Google Scholar]
  35. Shalaby M. R., Aggarwal B. B., Rindeknecht E., Svedersky L. P., Finkle B. S., Palladino M. A. (1985).Activation of human polymorphonuclear neutrophil functions by interferon-gammaand tumor necrosis factors. J Immunol 135:2069–2073
    [Google Scholar]
  36. Sharma R. A., Gescher A. J., Steward W. P. 2005; Curcumin: the story so far. Eur J Cancer 41:1955–1968 [CrossRef]
    [Google Scholar]
  37. Sittipunt C., Steinberg K. P., Ruzinski J. T., Myles C., Zhu S., Goodman R. B., Hudson L. D., Matalon S., Martin T. R. 2001; Nitric oxide and nitrotyrosine in the lungs of patientswith acute respiratory distress syndrome. Am J Respir Crit CareMed 163:503–510
    [Google Scholar]
  38. Strimpakos A. S., Sharma R. A. 2008; Curcumin: preventive and therapeutic properties in laboratory studiesand clinical trials. Antioxid Redox Signal 10:511–545 [CrossRef]
    [Google Scholar]
  39. Struve C., Krogfelt K. A. 2004; Pathogenic potential of environmental Klebsiella pneumoniae isolates. Environ Microbiol 6:584–590 [CrossRef]
    [Google Scholar]
  40. Tasaka S., Hasegawa N., Ishizaka A. 2002; Pharmacology of acute lung injury. Pulm PharmacolTher 15:83–95
    [Google Scholar]
  41. Tsai W. C., Strieter R. M., Zisman D. A., Wilkowski J. M., Bucknell K. A., Chen G., Standiford T. J. 1997; Nitric oxide is required for effective innate immunity against Klebsiellapneumoniae . Infect Immun 65:1870–1875
    [Google Scholar]
  42. Tsan M. F., White J. E., Michelsen P. B., Wong G. H. 1995; Pulmonary O2 toxicity: role ofendogenous tumor necrosis factor. Exp Lung Res 21:589–597 [CrossRef]
    [Google Scholar]
  43. Ukil A., Maity S., Karmakar S., Datta N., Vedasiromoni J. R., Das P. K. 2003; Curcumin, the majorcomponent of food flavour turmeric, reduces mucosal injury in trinitrobenzenesulphonic acid-induced colitis. Br J Pharmacol 139:209–218 [CrossRef]
    [Google Scholar]
  44. Wang L. F., Patel M., Razavi H. M., Weicker S., Joseph M. G., McCormack D. G., Mehta S. 2002; Roleof inducible nitric oxide synthase in pulmonary microvascular protein leakin murine sepsis. Am J Respir Crit Care Med 165:1634–1639 [CrossRef]
    [Google Scholar]
  45. Woo P. C. Y., Pau S. K. P., Yuen K.-Y. 2002; Macrolides as immunomodulatory agents. Curr MedChem Anti Inflamm Anti Allergy Agents 1:131–141
    [Google Scholar]
  46. Yadav V., Sharma S., Harjai K., Mohan H., Chhibber S. 2003; Induction and resolution of lobar pneumoniafollowing intranasal instillation with Klebsiella pneumoniae in mice. Indian J Med Res 118:47–52
    [Google Scholar]
  47. Yang J., Hooper W. C., Phillips D. J., Talkington D.F. 2002; Regulation of proinflammatory cytokinesin human lung epithelial cells infected with Mycoplasma pneumoniae . Infect Immun 70:3649–3655 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.016873-0
Loading
/content/journal/jmm/10.1099/jmm.0.016873-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error