1887

Abstract

Forty carbapenem-resistant isolates were recovered from 28 patients from various sites in an intensive care unit in Zhejiang Provincial People’s Hospital, China, over a 6 month period. PFGE analysis indicated that the 40 strains were all closely related. The MICs of carbapenems varied from 16 to >256 µg ml. Conjugation studies with resulted in the transfer of reduced carbapenem susceptibility from the original isolates. All and transconjugants produced carbapenemase 2 (KPC-2), and most of them produced TEM, SHV and CTX-M. Additionally, 27 isolates and 27 transconjugants carried the gene (25 were and 2 were ). harboured several plasmids, and was located on a 55 kb plasmid. SDS-PAGE and gene sequence analysis of OMPs suggested that porins in are expressed normally. The MICs of the carbapenems did not change in the presence of CCCP. Thus, production of KPC-2 appears to play an important role in resistance to carbapenems, although other mechanisms may be involved. The gene is associated with several antibiotic-resistance genes, such as , , and .

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2011-07-01
2019-12-11
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vol. , part 7, pp. 977 - 982

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Clinical details and resistance genes of carbapenem-resistant isolates.

Susceptibility results for isolates and transconjugants.



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