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Volume 59, Issue 6

Genotypic detection and molecular epidemiology of extended-spectrum -lactamase-producing and in a regional hospital in central Taiwan Free

Abstract

This study was conducted to detect the genes encoding extended-spectrum -lactamases (ESBLs) and determine the epidemiological relatedness of 69 and 33 isolates collected from a regional hospital in central Taiwan, mostly from inpatients ( 87.0 %; 88.0 %). The phenotypes of these isolates were examined according to the combination disc method recommended by the Clinical and Laboratory Standards Institute. Most of the ESBL-producing and isolates (98.6 % and 97 %, respectively) could be detected using cefotaxime discs with and without clavulanate. Genotyping was performed by PCR with type-specific primers. CTX-M-14 type (53.6 %) was the most prevalent ESBL among isolates while SHV type (57.6 %) was the most dominant among isolates. Six and three isolates did not carry genes encoding ESBLs of types TEM, SHV, CTX-M-3, CTX-M-14, CMY-2 and DHA-1. The co-existence of two or more kinds of ESBL in a single isolate was common, occurring in 40.6 % and 72.7 % of and isolates, respectively. PFGE analysis revealed that ESBL producers isolated in this setting were genetically divergent.

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Keyword(s): CAZ, ceftazidime , CLA, clavulanate , CTX, cefotaxime and ESBL, extended-spectrum β-lactamase
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2010-06-01
2024-04-20
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Genotypic detection and molecular epidemiology of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae in a regional hospital in central Taiwan
J. Med. Microbiol. 59, 665 (2010); https://doi.org/10.1099/jmm.0.015818-0
/content/journal/jmm/10.1099/jmm.0.015818-0
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