1887

Abstract

The objective of this study was to determine the distribution of staphylococcal cassette chromosome (SCC) elements in meticillin-resistant coagulase-negative staphylococci (MR-CoNS) isolated from a tertiary-care hospital in Mexico and to examine the relationship to drug resistance. Fifty selected MR-CoNS isolates collected from catheters (=15), blood (=15), bone (=9), bronchial lavage (=2) and urine (=2) and one isolate each from an abscess, cerebrospinal fluid, eye, pleural effusion, synovial fluid, tracheal aspirate and wound secretion were examined. Susceptibility testing was performed by the broth microdilution method. SCC types were determined by multiplex PCR and PFGE was carried out as described previously for . Among the MR-CoNS strains studied, the most frequently isolated species were (=26) and (=13). (=5), (=3), (=1), (=1) and the recently described species (=1) were also identified. The most frequent MR-CoNS genotype identified was SCC type IVa in isolates, which also showed a high diversity in their PFGE patterns. A clone was found that amplified both SCC III and V elements in five isolates examined. The single MR isolate harboured SCC type IVd and the single MR isolate harboured SCC type I. The carriage of SCC type III was associated with resistance or intermediate resistance to meropenem ( <0.05). These results confirm the high prevalence of SCC IVa and the high genetic diversity among MR-CoNS strains. As far as is known, this is the first report describing the newly identified possessing SCC IVd and harbouring SCC type I. MR-CoNS harbouring SCC type III were found to be more resistant to meropenem.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.015800-0
2010-03-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/jmm/59/3/323.html?itemId=/content/journal/jmm/10.1099/jmm.0.015800-0&mimeType=html&fmt=ahah

References

  1. Andrews, J. & Working Party for the British Society for Antimicrobial Chemotherapy ( 2008; ). BSAC Methods for Antimicrobial Susceptibility Testing. http://www.bsac.org.uk/_db/_documents/version_7.pdf.
  2. Baba, T., Kuwahara-Arai, K., Uchiyama, I., Takeuchi, F., Ito, T. & Hiramatsu, K. ( 2009; ). Complete genome sequence of Macrococcus caseolyticus strain JCSCS5402, [corrected] reflecting the ancestral genome of the human-pathogenic staphylococci. J Bacteriol 191, 1180–1190.[CrossRef]
    [Google Scholar]
  3. Bannerman, T. L. & Peacock, S. J. ( 2007; ). Staphylococcus, Micrococcus and other catalase-positive cocci. In Manual of Clinical Microbiology, 9th edn, pp. 390–404. Edited by P.R. Murray, E. J. Baron, J. H. Jorgensen, M. A. Pfaller & M. L. Landry. Washington, DC: American Society for Microbiology.
  4. Berglund, C. & Söderquist, B. ( 2008; ). The origin of a methicillin-resistant Staphylococcus aureus isolate at a neonatal ward in Sweden – possible horizontal transfer of a staphylococcal cassette chromosome mec between methicillin-resistant Staphylococcus haemolyticus and Staphylococcus aureus. Clin Microbiol Infect 14, 1048–1056.[CrossRef]
    [Google Scholar]
  5. Chongtrakool, P., Ito, T., Ma, X. X., Kondo, Y., Trakulsomboon, S., Tiensasitorn, C., Jamklang, M., Chavalit, T., Song, J. H. & Hiramatsu, K. ( 2006; ). Staphylococcal cassette chromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus strains isolated in 11 Asian countries: a proposal for a new nomenclature for SCCmec elements. Antimicrob Agents Chemother 50, 1001–1012.[CrossRef]
    [Google Scholar]
  6. CLSI ( 2009; ). Performance Standards for Antimicrobial Susceptibility Testing; Eighth Informational Supplement, CLSI document M100-S19. Wayne, PA: Clinical and Laboratory Standards Institute.
  7. Curtis, C. & Shetty, N. ( 2008; ). Recent trends and prevention of infection in the neonatal intensive care unit. Curr Opin Infect Dis 21, 350–356.[CrossRef]
    [Google Scholar]
  8. Falcone, M., Campanile, F., Giannella, M., Borbone, S., Stefani, S. & Venditti, M. ( 2007; ). Staphylococcus haemolyticus endocarditis: clinical and microbiologic analysis of 4 cases. Diagn Microbiol Infect Dis 57, 325–331.[CrossRef]
    [Google Scholar]
  9. Hanssen, A. M. & Ericson Sollid, J. U. ( 2006; ). SCCmec in staphylococci: genes on the move. FEMS Immunol Med Microbiol 46, 8–20.[CrossRef]
    [Google Scholar]
  10. Ibrahem, S., Salmenlinna, S., Virolainen, A., Kerttula, A. M., Lyytikäinen, O., Jägerroos, H., Broas, M. & Vuopio-Varkila, J. ( 2009; ). Carriage of methicillin-resistant staphylococci and their SCCmec types in a long-term-care facility. J Clin Microbiol 47, 32–37.[CrossRef]
    [Google Scholar]
  11. Ito, T., Katayama, Y., Asada, K., Mori, N., Tsutsumimoto, K., Tiensasitorn, C. & Hiramatsu, K. ( 2001; ). Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 45, 1323–1336.[CrossRef]
    [Google Scholar]
  12. Jamaluddin, T. Z., Kuwahara-Arai, K., Hisata, K., Terasawa, M., Cui, L., Baba, T., Sotozono, C., Kinoshita, S., Ito, T. & Hiramatsu, K. ( 2008; ). Extreme genetic diversity of methicillin-resistant Staphylococcus epidermidis strains disseminated among healthy Japanese children. J Clin Microbiol 46, 3778–3783.[CrossRef]
    [Google Scholar]
  13. Katayama, Y., Ito, T. & Hiramatsu, K. ( 2000; ). A new class of genetic element, staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus. Antimicrob Agents Chemother 44, 1549–1555.[CrossRef]
    [Google Scholar]
  14. Loïez, C., Wallet, F., Pischedda, P., Renaux, E., Senneville, E., Mehdi, N. & Courcol, R. J. ( 2007; ). First case of osteomyelitis caused by “Staphylococcus pettenkoferi”. J Clin Microbiol 45, 1069–1071.[CrossRef]
    [Google Scholar]
  15. Lyytikäinen, O., Lumio, J., Sarkkinen, H., Kolho, E., Kostiala, A., Ruutu, P. & Hospital Infection Surveillance Team ( 2002; ). Nosocomial bloodstream infections in Finnish hospitals during 1999–2000. Clin Infect Dis 35, e14–e19.[CrossRef]
    [Google Scholar]
  16. Ma, X. X., Ito, T., Tiensasitorn, C., Jamklang, M., Chongtrakool, P., Boyle-Vavra, S., Daum, R. S. & Hiramatsu, K. ( 2002; ). Novel type of staphylococcal cassette chromosome mec identified in community-acquired methicillin-resistant Staphylococcus aureus strains. Antimicrob Agents Chemother 46, 1147–1152.[CrossRef]
    [Google Scholar]
  17. Mombach Pinheiro Machado, A. B., Reiter, K. C., Paiva, R. M. & Barth, A. L. ( 2007; ). Distribution of staphylococcal cassette chromosome mec (SCCmec) types I, II, III and IV in coagulase-negative staphylococci from patients attending a tertiary hospital in southern Brazil. J Med Microbiol 56, 1328–1333.[CrossRef]
    [Google Scholar]
  18. Murchan, S., Kaufmann, M. E., Deplano, A., de Ryck, R., Struelens, M., Zinn, C. E., Fussing, V., Salmenlinna, S., Vuopio-Varkila, J. & other authors ( 2003; ). Harmonization of pulsed-field gel electrophoresis protocols for epidemiological typing of strains of methicillin-resistant Staphylococcus aureus, a single approach developed by consensus in 10 European laboratories and its application for tracing the spread of related strains. J Clin Microbiol 41, 1574–1585.[CrossRef]
    [Google Scholar]
  19. Musser, J. M. & Kapur, V. ( 1992; ). Clonal analysis of methicillin-resistant Staphylococcus aureus strains from intercontinental sources: association of the mec gene with divergent phylogenetic lineages implies dissemination by horizontal transfer and recombination. J Clin Microbiol 30, 2058–2063.
    [Google Scholar]
  20. Pei, Z., Bini, E. J., Yang, L., Zhou, M., Francois, F. & Blaser, M. J. ( 2004; ). Bacterial biota in the human distal esophagus. Proc Natl Acad Sci U S A 101, 4250–4255.[CrossRef]
    [Google Scholar]
  21. Ruppé, E., Barbier, F., Mesli, Y., Maiga, A., Cojocaru, R., Benkhalfat, M., Benchouk, S., Hassaine, H., Maiga, I. & other authors ( 2009; ). Diversity of staphylococcal cassette chromosome mec structures in methicillin-resistant Staphylococcus epidermidis and Staphylococcus haemolyticus strains among outpatients from four countries. Antimicrob Agents Chemother 53, 442–449.[CrossRef]
    [Google Scholar]
  22. Savini, V., Catavitello, C., Pompetti, F., Passeri, C., Di Zacomo, S., Esattore, F., Iacone, A. & D’Antonio, D. ( 2008; ). Contamination of a donated platelet unit by Staphylococcus pasteuri. J Infect 57, 494–496.[CrossRef]
    [Google Scholar]
  23. Savini, V., Catavitello, C., Carlino, D., Pompilio, A., Balbinot, A., Piccolomini, R., Di Bonaventura, G. & D’Antonio, D. ( 2009a; ). Staphylococcus pasteuri bacteraemia in patient with leukaemia. J Clin Pathol 62, 957–958.[CrossRef]
    [Google Scholar]
  24. Savini, V., Bianco, A., Catavitello, C., Balbinot, A., Pompilio, A., Piccolomini, R., di Bonaventura, G. & D’Antonio, D. ( 2009b; ). Meticillin-heteroresistant Staphylococcus pasteuri from an apheresis platelet product. J Med Microbiol 58, 1527–1528.[CrossRef]
    [Google Scholar]
  25. Song, S. H., Park, J. S., Kwon, H. R., Kim, S. H., Kim, H. B., Chang, H. E., Park, K. U., Song, J. & Kim, E. C. ( 2009; ). Human bloodstream infection caused by Staphylococcus pettenkoferi. J Med Microbiol 58, 270–272.[CrossRef]
    [Google Scholar]
  26. Tenover, F. C., Arbeit, R. D., Goering, R. V., Mickelsen, P. A., Murray, B. E., Persing, D. H. & Swaminathan, B. ( 1995; ). Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33, 2233–2239.
    [Google Scholar]
  27. Trülzsch, K., Rinder, H., Trcek, J., Bader, L., Wilhelm, U. & Heesemann, J. ( 2002; ). Staphylococcus pettenkoferi,” a novel staphylococcal species isolated from clinical specimens. Diagn Microbiol Infect Dis 43, 175–182.[CrossRef]
    [Google Scholar]
  28. Trülzsch, K., Grabein, B., Schumann, P., Mellmann, A., Antonenka, U., Heesemann, J. & Becker, K. ( 2007; ). Staphylococcus pettenkoferi sp. nov., a novel coagulase-negative staphylococcal species isolated from human clinical specimens. Int J Syst Evol Microbiol 57, 1543–1548.[CrossRef]
    [Google Scholar]
  29. Vannuffel, P., Gigi, J., Ezzedine, H., Vandercam, B., Delmee, M., Wauters, G. & Gala, J. L. ( 1995; ). Specific detection of methicillin-resistant Staphylococcus species by multiplex PCR. J Clin Microbiol 33, 2864–2867.
    [Google Scholar]
  30. Viale, P. & Stefani, S. ( 2006; ). Vascular catheter-associated infections: a microbiological and therapeutic update. J Chemother 18, 235–249.[CrossRef]
    [Google Scholar]
  31. Wisplinghoff, H., Rosato, A. E., Enright, M. C., Noto, M., Craig, W. & Archer, G. L. ( 2003; ). Related clones containing SCCmec type IV predominate among clinically significant Staphylococcus epidermidis isolates. Antimicrob Agents Chemother 47, 3574–3579.[CrossRef]
    [Google Scholar]
  32. Zhang, K., McClure, J. A., Elsayed, S., Louie, T. & Conly, J. M. ( 2005; ). Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. J Clin Microbiol 43, 5026–5033.[CrossRef]
    [Google Scholar]
  33. Zhang, K., McClure, J. A., Elsayed, S. & Conly, J. M. ( 2009; ). Novel staphylococcal cassette chromosome mec type, tentatively designated type VIII, harboring class A mec and type 4 ccr gene complexes in a Canadian epidemic strain of methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 53, 531–540.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.015800-0
Loading
/content/journal/jmm/10.1099/jmm.0.015800-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error