1887

Abstract

Studies based on the analysis of housekeeping genes indicate that and all species, except for type 13, belong to a single species. This study analysed the phenotypicand genotypic characteristics of 23 . strains isolatedin different countries from faecal specimens taken from children with diarrhoea.Strains were identified using the VITEK system and typed with rabbit seraobtained against 186 somatic and 53 flagellar antigens andagainst 45 somatic antigens. Biochemical analysis of thesestrains showed a typical profile with a defined reactionagainst both . O179 and . 16 somatic antisera. Agglutination assays for flagellar antigens showed aresponse against H2 in 7 (30 %) strains, H10 in 2 (9 %)strains, H32 in 12 (52 %) strains and H34 in 2 (9 %)strains, demonstrating 4 serotypes associated with this new somatic antigen64474. A serum against one of these . strains (64474)was prepared. Absorption assays of . 16 and . 64474 antisera with O179 antigen removedthe agglutination response against this O179 antigen completely, while theagglutination titres against both . 16 and 64474 remained the same. Four (17 %) . strains showed antimicrobial resistance to piperacillinonly, one (4 %) to piperacillin and trimethoprim/sulfamethoxazole,one (4 %) to ciprofloxacin, nitrofurantoin and piperacillin,and two (9 %) strains were resistant to ciprofloxacin,norfloxacin, ofloxacin, piperacillin and trimethoprim/sulfamethoxazole.With regards to PCR assays, one (4 %) of the strainswas positive for gene , one (4 %)for , two (9 %) for , one (4 %)for , two (9 %) for andthree (13 %) for . The genecluster in the strains was analysed by RFLP and comparedwith the gene cluster obtained from 16. The -RFLPpatterns for all 23 . strains were similar to thoseobtained for . 16. The results from PCR tests todetect genes (encoding O unit flippase)and (encoding polymerase) belonging to a cluster relatedto the biosynthesis of the 16-specific O antigen were positivein 21 (91 %) and 22 (96 %)of the strains, respectively. PCR assays to detect . virulence genes were also performed. These assays detected enterotoxigenic . genes in 12 of the strains (52 %), in 4 (17 %), 1 in 6 (26 %), 1 in 1 (4 %), 3 in 3 (13 %), 13 in 9 (39 %) and 14 in 5 (22 %)of the strains. Results from the PFGE analyses confirmed the wide geographicaldistribution of these strains suggesting that 64474 : H2, 64474 : H10,64474 : H32 and 64474 : H34 are new serotypesof . strains with a defined virulence capacity, andshare a common O antigen with 16.

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2010-04-01
2024-03-19
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