@article{mbs:/content/journal/jmm/10.1099/jmm.0.015503-0, author = "Poppert, Sven and Riecker, Melanie and Wellinghausen, Nele and Frickmann, Hagen and Essig, Andreas", title = "Accelerated identification of Staphylococcus aureus from blood cultures by a modified fluorescence in situ hybridization procedure", journal= "Journal of Medical Microbiology", year = "2010", volume = "59", number = "1", pages = "65-68", doi = "https://doi.org/10.1099/jmm.0.015503-0", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.015503-0", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", keywords = "PNA, peptide nucleic acid", keywords = "CoNS, coagulase-negative staphylococci", keywords = "FISH, fluorescence in situ hybridization", abstract = "This study evaluated fluorescence in situ hybridization (FISH) for rapid identification of Staphylococcus aureus and coagulase-negative staphylococci (CoNS) directly from blood cultures. Initially, 360 blood cultures containing Gram-positive cocci were investigated by a previously described microwave-FISH procedure: 44/49 (89.8 %) S. aureus and 298/299 (99.7 %) CoNS were correctly identified. Because FISH proved useful and reliable but handling was found to be inconvenient, the method was modified by employing a recently developed slide chamber. This reduced the time required from 60 to 30 min. The simplified execution allowed integration of the method into the workflow of a routine laboratory without difficulty. The modified method proved to be highly reliable, identifying 37/37 (100 %) S. aureus and 169/172 (98.2 %) CoNS directly from blood cultures.", }