1887

Abstract

Molecular diagnostic methods are of potential value in identifying tuberculosis (TB) and drug resistance where tissue specimens have been submitted for histology but not for microbiological culture. All paraffin-embedded tissue (PET) specimens (=60) referred to a single national centre over a 42 month study period were analysed using the INNO-LiPA Rif.TB assay; 29/60 patients had been reported to the UK Enhanced Tuberculosis Surveillance database with a diagnosis of TB based on clinical, radiological and histological evidence. (MTB) DNA was detectable in 5 out of 29 reported TB cases (17.2 %); 12 out of 29 of the reported TB cases had a positive MTB culture from a secondary clinical specimen and MTB DNA was detectable in 2 of the 12 (16.7 %) laboratory-confirmed TB cases. Referring clinicians should be aware of the limitations of this assay on PETs, and should request molecular testing only in patients with a high clinical probability of TB and when acid-fast bacilli are seen in tissue specimens.

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2010-05-01
2019-11-18
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References

  1. Alvarado-Esquivel, C., García-Corral, N., Carrero-Dominguez, D., Enciso-Moreno, J. A., Gurrola-Morales, T., Portillo-Gómez, L., Rossau, R. & Mijs, W. ( 2009; ). Molecular analysis of Mycobacterium isolates from extrapulmonary specimens obtained from patients in Mexico. BMC Clin Pathol 9, 1 [CrossRef]
    [Google Scholar]
  2. De Beenhouwer, H., Lhiang, Z., Jannes, W., Nfijs, L., Machtelinckx, L., Rossau, R., Traore, H. & Portaels, F. ( 1995; ). Rapid detection of rifampin resistance in sputum and biopsy specimens from tuberculosis patients by PCR and line probe assay. Tuber Lung Dis 76, 425–430.[CrossRef]
    [Google Scholar]
  3. Greer, C. E., Peterson, S. L., Kiviat, N. B. & Manos, M. M. ( 1991; ). PCR amplification from paraffin-embedded tissues – effects of fixative and fixation time. Am J Clin Pathol 95, 117–124.
    [Google Scholar]
  4. HPA ( 2004; ). Annual Report on Tuberculosis Cases Reported in 2001 in England, Wales and Northern Ireland. London: Health Protection Agency. http://www.hpa.org.uk/web/HPAwebFile/HPAweb_C/1194947356221
  5. Honoré-Bouakline, S., Vincensini, J. P., Giacuzzo, V., Lagrange, P. H. & Herrman, J. L. ( 2003; ). Rapid diagnosis of extrapulmonary tuberculosis by PCR: impact of sample preparation and DNA extraction. J Clin Microbiol 41, 2323–2329.[CrossRef]
    [Google Scholar]
  6. NICE ( 2006; ). Tuberculosis: Clinical Diagnosis and Management of Tuberculosis, and Measures for its Prevention and Control. London: National Institute for Health and Clinical Excellence. http://www.nice.org.uk/nicemedia/pdf/CG033niceguideline.pdf
  7. Pfyffer, G. E., Kissling, P., Jahn, E. M., Welscher, H. M., Salfinger, M. & Weber, R. ( 1996; ). Diagnostic performance of amplified Mycobacterium tuberculosis direct test with cerebrospinal fluid, other nonrespiratory, and respiratory specimens. J Clin Microbiol 34, 834–841.
    [Google Scholar]
  8. Popper, H. H., Winter, E. & Hofler, G. ( 1994; ). DNA of Mycobacterium tuberculosis in formalin-fixed, paraffin-embedded tissue in tuberculosis and sarcoidosis detected by polymerase chain reaction. Am J Clin Pathol 101, 738–741.
    [Google Scholar]
  9. Rish, J. A., Eisenach, K. D., Cave, M. D., Reddy, M. V., Gangadharam, P. R. J. & Bates, J. H. ( 1996; ). Polymerase chain reaction of Mycobacterium tuberculosis in formalin-fixed tissue. Am J Respir Crit Care Med 153, 1419–1423.[CrossRef]
    [Google Scholar]
  10. Rossau, R., Traore, H., de Beenhouwer, H., Mijs, W., Jannes, G., de Rijk, P. & Portaels, F. ( 1997; ). Evaluation of the INNO-LiPA Rif. TB assay, a reverse hybridization assay for the simultaneous detection of Mycobacterium tuberculosis complex and its resistance to rifampin. Antimicrob Agents Chemother 41, 2093–2098.
    [Google Scholar]
  11. Sam, I. C., Drobniewski, F., More, P., Kemp, M. & Brown, T. ( 2006; ). Mycobacterium tuberculosis and rifampin resistance, in the United Kingdom. Emerg Infect Dis 12, 752–759.[CrossRef]
    [Google Scholar]
  12. Shibata, D. ( 1994; ). Extraction of DNA from paraffin-embedded tissue for analysis by polymerase chain reaction: new tricks from an old friend. Hum Pathol 25, 561–563.[CrossRef]
    [Google Scholar]
  13. Skenders, G., Fry, A. M., Prokopovica, I., Greckoseja, S., Broka, L., Metchock, B, Holtz, T. H., Wells, C. D. & Leimane, V. ( 2005; ). Multidrug-resistant tuberculosis detection, Latvia. Emerg Infect Dis 11, 1461–1463.[CrossRef]
    [Google Scholar]
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