1887

Abstract

A total of 95 isolates, belonging to 33 species of five dermatophyte genera, i.e. (15 species), (two), (one), (three) and (12), were studied using internal transcribed spacer 2 (ITS2)-PCR-RFLP analysis (ITS2-RFLP), consisting of amplification of the ITS2 region, restriction digestion with UI (CG/CG) and restriction fragment length determination by capillary electrophoresis. ITS2-RFLP analysis proved to be most useful for identification of species of the genera , and , but could not distinguish between several species. The identification results are in agreement with established and recent taxonomical insights into the dermatophytes; for example, highly related species also had closely related and sometimes difficult-to-discriminate ITS2-RFLP patterns. In some cases, several ITS2-RFLP groups could be distinguished within species, again mostly in agreement with the taxonomic delineations of subspecies and/or genomovars, confirming the relevance of ITS2-RFLP analysis as an identification technique and as a useful taxonomic approach.

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2010-01-01
2019-11-21
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References

  1. Arabatzis, M., Xylouri, E., Frangiadaki, I., Tzimogianni, A., Milioni, A., Arsenis, G. & Velegraki, A. ( 2006; ). Rapid detection of Arthroderma vanbreuseghemii in rabbit skin specimens by PCR-RFLP. Vet Dermatol 17, 322–326.[CrossRef]
    [Google Scholar]
  2. Arabatzis, M., Bruijnesteijn van Coppenraet, L. E., Kuijper, E. J., de Hoog, G. S., Lavrijsen, A. P., Templeton, K., van der Raaij-Helmer, E. M., Velegraki, A., Gräser, Y. & Summerbell, R. C. ( 2007; ). Diagnosis of common dermatophyte infections by a novel multiplex real-time polymerase chain reaction detection/identification scheme. Br J Dermatol 157, 681–689.[CrossRef]
    [Google Scholar]
  3. Baele, M., Storms, V., Haesebrouck, F., Devriese, L. A., Gillis, M., Verschraegen, G., de Baere, T. & Vaneechoutte, M. ( 2001; ). Application and evaluation of the interlaboratory reproducibility of tRNA intergenic length polymorphism analysis (tDNA-PCR) for identification of Streptococcus species. J Clin Microbiol 39, 1436–1442.[CrossRef]
    [Google Scholar]
  4. De Baere, T., Claeys, G., Swinne, D., Verschraegen, G., Muylaert, A., Massonet, C. & Vaneechoutte, M. ( 2002; ). Identification of cultured isolates of clinically important yeast species using fluorescent fragment length analysis of the amplified internally transcribed rRNA spacer 2 region (ITS2). BMC Microbiol 2, 21 [CrossRef]
    [Google Scholar]
  5. De Baere, T., Van Keerberghen, A., Van Hauwe, P., De Beenhouwer, H., Boel, A., Verschraegen, G., Claeys, G. & Vaneechoutte, M. ( 2005; ). An interlaboratory comparison of ITS2-PCR for the identification of yeasts, using the ABI Prism 310 and CEQ8000 capillary electrophoresis systems. BMC Microbiol 5, 14 [CrossRef]
    [Google Scholar]
  6. Erhard, M., Hipler, U. C., Burmester, A., Brakhage, A. A. & Wostemeyer, J. ( 2008; ). Identification of dermatophyte species causing onychomycosis and tinea pedis by MALDI-TOF mass spectrometry. Exp Dermatol 17, 356–361.[CrossRef]
    [Google Scholar]
  7. Gemmer, C. M., DeAngelis, Y. M., Theelen, B., Boekhout, T. & Dawson, T. L., Jr ( 2002; ). Fast, noninvasive method for molecular detection and differentiation of Malassezia yeast species on human skin and application of the method to dandruff microbiology. J Clin Microbiol 40, 3350–3357.[CrossRef]
    [Google Scholar]
  8. Gräser, Y., El Fari, M., Vilgalys, R., Kuijpers, A. F., De Hoog, G. S., Presber, W. & Tietz, H. ( 1999; ). Phylogeny and taxonomy of the family Arthrodermataceae (dermatophytes) using sequence analysis of the ribosomal ITS region. Med Mycol 37, 105–114.[CrossRef]
    [Google Scholar]
  9. Gräser, Y., Kuijpers, A. F., Presber, W. & de Hoog, G. S. ( 2000; ). Molecular taxonomy of the Trichophyton rubrum complex. J Clin Microbiol 38, 3329–3336.
    [Google Scholar]
  10. Nenoff, P., Herrmann, J. & Gräser, Y. ( 2007; ). Trichophyton mentagrophytes sive interdigitale? A dermatophyte in the course of time. J Dtsch Dermatol Ges 5, 198–202.[CrossRef]
    [Google Scholar]
  11. O'Donnell, K., Cigelnik, E., Weber, N. S. & Trappe, J. M. ( 1997; ). Phylogenetic relationships among ascomycetous truffles and the true and false morels inferred from 18S and 28S ribosomal DNA sequence analysis. Mycologia 89, 48–65.[CrossRef]
    [Google Scholar]
  12. Summerbell, R. C., Haugland, R. A., Li, A. & Gupta, A. K. ( 1999; ). rRNA gene internal transcribed spacer 1 and 2 sequences of asexual, anthropophilic dermatophytes related to Trichophyton rubrum. J Clin Microbiol 37, 4005–4011.
    [Google Scholar]
  13. Summerbell, R. C., Moore, M. K., Starink-Willemse, M. & Van Iperen, A. ( 2007; ). ITS barcodes for Trichophyton tonsurans and T. equinum. Med Mycol 45, 193–200.[CrossRef]
    [Google Scholar]
  14. Turenne, C. Y., Sanche, S. E., Hoban, D. J., Karlowsky, J. A. & Kabani, A. M. ( 1999; ). Rapid identification of fungi by using the ITS2 genetic region and an automated fluorescent capillary electrophoresis system. J Clin Microbiol 37, 1846–1851.
    [Google Scholar]
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