1887

Abstract

The pathogenicity island (PAI) has been reported to be the major virulence determinant in -related diseases. In the present study, the diversity of the gene and the integrity of the PAI in 158 strains from Varanasi (North India) and Hyderabad (South India) were studied by amplifying the gene (∼3.5 kb), followed by PCR-RFLP analysis. The results revealed significant differences in the gene and the integrity of the PAI between North and South Indian isolates. Of 158 isolates, 40 (34.8 %) from Varanasi and 20 (46.5 %) from Hyderabad were found to carry an intact PAI. A partially deleted PAI was present in 75 (65.2 %) isolates from Varanasi and 23 (53.5 %) from Hyderabad. None of the isolates showed complete deletion of the PAI. Differences in the 5′ and 3′ regions were also noted, and 11 isolates (8 from Varanasi and 3 from Hyderabad) that were negative with primers for the 5′ region turned out to be positive with primers for the 3′ variable region. It is tentatively concluded that the 3′ variable region may be a better marker for typing. The results also showed that the majority of the isolates harboured the Western-type EPIYA motif. PCR-RFLP analysis of the gene showed 29 distinguishable digestion patterns, and cluster analysis of RFLP types from a random selection of 32 isolates placed all of the isolates into 5 groups. These results demonstrate that significant differences in the PAI occur among isolates from North and South India, and that RFLP of could be employed for elucidating genetic variations among various isolates of .

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2010-01-01
2019-11-18
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