1887

Abstract

, carried by ticks, is one of the most significant human pathogens, causing Lyme disease. As there is no standardized PCR method for detection and identification of spirochaete DNA, we carried out a comparative analysis using a set of complementary primers for three regions in the genomic DNA of these bacteria (genes and and the non-coding region). DNA extracted from 579 ticks was subjected to nested PCR. DNA of the examined spirochaetes was detected in 43 (7.4 %) lysates when the gene was used as a molecular marker, in 7 (1.2 %) lysates when using primers complementary to the gene, and in 12 (2.1 %) lysates using primers complementary to the non-coding sequence. RFLP analysis based on the gene helped identify species from the complex ( , , , ), detect co-infections, and also identify . Therefore, the gene is the most sensitive and specific molecular marker for the detection and identification of spirochaetes in

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2010-03-01
2019-10-20
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