1887

Abstract

In order to identify the source of infections caused by dermatophytes, as well as the pathogen transmission pathway, there is a need to determine methods that allow detailed genetic differentiation of the strains within the dermatophyte genera. In this work, a PCR melting profile (PCR-MP) technique based on the ligation of adaptors and the difference in melting temperatures of DNA restriction fragments was used for the first time for intraspecies genotyping of dermatophytes. Clinical isolates and reference strains of dermatophytes isolated from skin, scalp, toenails and fingernails were used for this study. PCR-MP and random amplification of polymorphic DNA (RAPD) were used to type 11 isolates of , 40 isolates of and 14 isolates of . The results distinguished five types (containing one subtype) characteristic for and seven types characteristic for using the PCR-MP technique. Analysis conducted using RAPD revealed five types for and four types for isolates. No differentiation was observed for the isolates with either method. These results demonstrate that PCR-MP is a reliable method for the differentiation of and strains and yields a discriminatory power that is at least equal to that of RAPD.

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2010-02-01
2019-10-16
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