1887

Abstract

The slow growth of hepatitis A virus (HAV) in cell culture is one of the primary pitfalls in the development of sensitive and rapid methods for the detection and quantification of HAV and associated neutralizing antibodies. Currently, assays frequently require 8 days or more to detect and quantify the presence of HAV neutralizing antibodies. This study describes a rapid immunoassay that allowed the detection of anti-HAV neutralizing antibodies in only 3 days. This microplate-based enzymic assay may be applicable in virological diagnostics, in evaluating the immunogenicity of HAV vaccines and in quantifying neutralizing antibodies during the course of HAV infection.

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2009-11-01
2019-10-15
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References

  1. Beales, L. P., Wood, D. J., Minor, P. D. & Saldanha, J. A. ( 1996; ). A novel cytopathic microtitre plate assay for hepatitis A virus and anti-hepatitis A neutralizing antibodies. J Virol Methods 59, 147–154.[CrossRef]
    [Google Scholar]
  2. Bishop, N. E. & Anderson, D. A. ( 1997; ). Early interactions of hepatitis A virus with cultured cells: viral elution and the effect of pH and calcium ions. Arch Virol 142, 2161–2178.[CrossRef]
    [Google Scholar]
  3. Bordignon, J., Comin, F., Ferreira, S. C., Caporale, G. M., Lima Filho, J. H. & Zanetti, C. R. ( 2002a; ). Calculating rabies virus neutralizing antibodies titres by flow cytometry. Rev Inst Med Trop Sao Paulo 44, 151–154.[CrossRef]
    [Google Scholar]
  4. Bordignon, J., Pires Ferreira, S. C., Medeiros Caporale, G. M., Carrieri, M. L., Kotait, I., Lima, H. C. & Zanetti, C. R. ( 2002b; ). Flow cytometry assay for intracellular rabies virus detection. J Virol Methods 105, 181–186.[CrossRef]
    [Google Scholar]
  5. Brack, K., Frings, W., Dotzauer, A. & Vallbracht, A. ( 1998; ). A cytopathogenic, apoptosis-inducing variant of hepatitis A virus. J Virol 72, 3370–3376.
    [Google Scholar]
  6. Brown, F. ( 1989; ). The classification and nomenclature of viruses: summary of results of meetings of the International Committee on Taxonomy of Viruses in Edmonton, Canada 1987. Intervirology 30, 181–186.
    [Google Scholar]
  7. Cao, J., Meng, S., Li, C., Ji, Y., Meng, Q., Zhang, Q., Liu, F., Li, J., Bi, S. & other authors ( 2008; ). Efficient neutralizing activity of cocktailed recombinant human antibodies against hepatitis A virus infection in vitro and in vivo. J Med Virol 80, 1171–1180.[CrossRef]
    [Google Scholar]
  8. Chen, H. & Cantrell, C. R. ( 2006; ). Prevalence and factors associated with self-reported vaccination rates among US adults at high risk of vaccine-preventable hepatitis. Curr Med Res Opin 22, 2489–2496.[CrossRef]
    [Google Scholar]
  9. Emerson, S. U., Huang, Y. K. & Purcell, R. H. ( 1993; ). 2B and 2C mutations are essential but mutations throughout the genome of HAV contribute to adaptation to cell culture. Virology 194, 475–480.[CrossRef]
    [Google Scholar]
  10. Endo, K., Takahashi, M., Masuko, K., Inoue, K., Akahane, Y. & Okamoto, H. ( 2007; ). Full-length sequences of subgenotype IIIA and IIIB hepatitis A virus isolates: characterization of genotype III HAV genomes. Virus Res 126, 116–127.[CrossRef]
    [Google Scholar]
  11. Gauss-Muller, V., Lottspeich, F. & Deinhardt, F. ( 1986; ). Characterization of hepatitis A virus structural proteins. Virology 155, 732–736.[CrossRef]
    [Google Scholar]
  12. Gosert, R., Egger, D. & Bienz, K. ( 2000; ). A cytopathic and a cell culture adapted hepatitis A virus strain differ in cell killing but not in intracellular membrane rearrangements. Virology 266, 157–169.[CrossRef]
    [Google Scholar]
  13. Jelic, O., Fornet-Sapcevski, J., Kovacevic, L., Pandak, N. & Jelic, D. ( 1990; ). Recurrences of viral hepatitis A. Acta Med Iugosl 44, 565–576.
    [Google Scholar]
  14. Kim, S. J., Jang, M. H., Stapleton, J. T., Yoon, S. O., Kim, K. S., Jeon, E. S. & Hong, H. J. ( 2004; ). Neutralizing human monoclonal antibodies to hepatitis A virus recovered by phage display. Virology 318, 598–607.[CrossRef]
    [Google Scholar]
  15. Konduru, K., Virata-Theimer, M. L., Yu, M. Y. & Kaplan, G. G. ( 2008; ). A simple and rapid hepatitis A virus (HAV) titration assay based on antibiotic resistance of infected cells: evaluation of the HAV neutralization potency of human immune globulin preparations. Virol J 5, 155 [CrossRef]
    [Google Scholar]
  16. Lemon, S. M., Murphy, P. C., Shields, P. A., Ping, L. H., Feinstone, S. M., Cromeans, T. & Jansen, R. W. ( 1991; ). Antigenic and genetic variation in cytopathic hepatitis A virus variants arising during persistent infection: evidence for genetic recombination. J Virol 65, 2056–2065.
    [Google Scholar]
  17. Lemon, S. M., Jansen, R. W. & Brown, E. A. ( 1992; ). Genetic, antigenic and biological differences between strains of hepatitis A virus. Vaccine 10, S40–S44.[CrossRef]
    [Google Scholar]
  18. Nainan, O. V., Xia, G., Vaughan, G. & Margolis, H. S. ( 2006; ). Diagnosis of hepatitis A virus infection: a molecular approach. Clin Microbiol Rev 19, 63–79.[CrossRef]
    [Google Scholar]
  19. Pinto, R. M., Aragones, L., Costafreda, M. I., Ribes, E. & Bosch, A. ( 2007; ). Codon usage and replicative strategies of hepatitis A virus. Virus Res 127, 158–163.[CrossRef]
    [Google Scholar]
  20. Sanchez, G., Populaire, S., Butot, S., Putallaz, T. & Joosten, H. ( 2006; ). Detection and differentiation of human hepatitis A strains by commercial quantitative real-time RT-PCR tests. J Virol Methods 132, 160–165.[CrossRef]
    [Google Scholar]
  21. Shouval, D., Ashur, Y., Adler, R., Lewis, J. A., Armstrong, M. E., Davide, J. P., McGuire, B., Kuter, B., Brown, L. & other authors ( 1993; ). Single and booster dose responses to an inactivated hepatitis A virus vaccine: comparison with immune serum globulin prophylaxis. Vaccine 11, S9–S14.[CrossRef]
    [Google Scholar]
  22. Siegl, G., Dechastonay, J. & Kronauer, G. ( 1984; ). Propagation and assay of hepatitis A virus in vitro. J Virol Methods 9, 53–67.[CrossRef]
    [Google Scholar]
  23. Stapleton, J. T., Raina, V., Winokur, P. L., Walters, K., Klinzman, D., Rosen, E. & McLinden, J. H. ( 1993; ). Antigenic and immunogenic properties of recombinant hepatitis A virus 14S and 70S subviral particles. J Virol 67, 1080–1085.
    [Google Scholar]
  24. Yeh, H. Y., Hwang, Y. C., Yates, M. V., Mulchandani, A. & Chen, W. ( 2008; ). Detection of hepatitis A virus by using a combined cell culture–molecular beacon assay. Appl Environ Microbiol 74, 2239–2243.[CrossRef]
    [Google Scholar]
  25. Zahn, J., Vallbracht, A. & Flehmig, B. ( 1984; ). Hepatitis A-virus in cell culture. V. Neutralizing antibodies against hepatitis A-virus. Med Microbiol Immunol 173, 9–17.[CrossRef]
    [Google Scholar]
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