1887

Abstract

putative virulence factors can undergo a continuously evolving mechanism as an approach to bacterial adaptation to the host changing environment during chronic infection. , and genetic diversity among isolates from multiple biopsies (niches) from the antrum and corpus of 40 patients was investigated. A set of 229 isolates was examined. Direct DNA sequence analysis of amplified fragments was used to study ‘on/off’ expression status as well as the presence of C or T insertion in that originates a continuous () gene. alleles were identified by multiplex PCR. Different inter-niches CT repeat patterns were observed in nine patients; in six of these, ‘on’ and ‘off’ mixed patterns were found. In three of these nine patients, different alleles were also observed in a single host. Inter-niche differences involved the absence and presence of and/or or mutations in , including those that may originate a non-functional gene, and they were also present in two patients with mixed CT patterns and in another seven patients. Evidence of mixed infection was observed in two patients only. In conclusion, and genes showed similar inter-niche variability, occurring in approximately 1/4 patients. Conversely, allele microevolution seemed to be a less common event, occurring in approximately 1/10 patients, probably due to the mechanism that this gene evolves ‘’.

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2010-01-01
2019-11-22
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References

  1. Alvi, A., Devi, S. M., Ahmed, I., Hussain, M. A., Rizwan, M., Lamouliatte, H., Mégraud, F. & Ahmed, N. ( 2007; ). Microevolution of Helicobacter pylori type IV secretion systems in an ulcer disease patient over a ten-year period. J Clin Microbiol 45, 4039–4043.[CrossRef]
    [Google Scholar]
  2. Arachchi, H. S., Kalra, V., Lal, B., Bhatia, V., Baba, C. S., Chakravarthy, S., Rohatgi, S., Sarma, P., Mishra, M. V. & other authors ( 2007; ). Prevalence of duodenal ulcer-promoting gene (dupA) of Helicobacter pylori in patients with duodenal ulcer in North Indian population. Helicobacter 12, 591–597.[CrossRef]
    [Google Scholar]
  3. Aviles-Jimenez, F., Letley, D. P., Gonzalez-Valencia, G., Salama, N., Torres, J. & Atherton, J. C. ( 2004; ). Evolution of the Helicobacter pylori vacuolating cytotoxin in a human stomach. J Bacteriol 186, 5182–5185.[CrossRef]
    [Google Scholar]
  4. Carroll, I. M., Ahmed, N., Beesley, S. M., Khan, A. A., Ghousunnissa, S., Moráin, C. A., Habibullah, C. M. & Smyth, C. J. ( 2004; ). Microevolution between paired antral and paired antrum and corpus Helicobacter pylori isolates recovered from individual patients. J Med Microbiol 53, 669–677.[CrossRef]
    [Google Scholar]
  5. Chiarini, A., Calà, C., Bonura, C., Gullo, A., Giuliana, G., Peralta, S., D'Arpa, F. & Giammanco, A. ( 2008; ). Prevalence of virulence-associated genotypes of Helicobacter pylori and correlation with severity of gastric pathology in patients from western Sicily, Italy. Eur J Clin Microbiol Infect Dis 28, 437–446.
    [Google Scholar]
  6. Cooke, C. L., Huff, J. L. & Solnick, J. V. ( 2005; ). The role of genome diversity and immune evasion in persistent infection with Helicobacter pylori. FEMS Immunol Med Microbiol 45, 11–23.[CrossRef]
    [Google Scholar]
  7. Dossumbekova, A., Prinz, C., Mages, J., Lang, R., Kusters, J. G., Van Vliet, A. H., Reindl, W., Backert, S., Saur, D. & other authors ( 2006; ). Helicobacter pylori HopH (OipA) and bacterial pathogenicity: genetic and functional genomic analysis of hopH gene polymorphisms. J Infect Dis 194, 1346–1355.[CrossRef]
    [Google Scholar]
  8. Douraghi, M., Mohammadi, M., Oghalaie, A., Abdirad, A., Mohagheghi, M. A., Hosseini, M. E., Zeraati, H., Ghasemi, A., Esmaieli, M. & Mohajerani, N. ( 2008; ). dupA as a risk determinant in Helicobacter pylori infection. J Med Microbiol 57, 554–562.[CrossRef]
    [Google Scholar]
  9. Erzin, Y., Koksal, V., Altun, S., Dobrucali, A., Aslan, M., Erdamar, S., Dirican, A. & Kocazeybek, B. ( 2006; ). Prevalence of Helicobacter pylori vacA, cagA, cagE, iceA, babA2 genotypes and correlation with clinical outcome in Turkish patients with dyspepsia. Helicobacter 11, 574–580.[CrossRef]
    [Google Scholar]
  10. Falush, D., Kraft, C., Taylor, N. S., Correa, P., Fox, J. G., Achtman, M. & Suerbaum, S. ( 2001; ). Recombination and mutation during long-term gastric colonization by Helicobacter pylori: estimates of clock rates, recombination size, and minimal age. Proc Natl Acad Sci U S A 98, 15056–15061.[CrossRef]
    [Google Scholar]
  11. Faundez, G., Troncoso, M. & Figueroa, G. ( 2002; ). cagA and vacA in strains of Helicobacter pylori from ulcer and non-ulcerative dyspepsia patients. BMC Gastroenterol 2, 20–24.[CrossRef]
    [Google Scholar]
  12. Go, M. F., Kapur, V., Graham, D. Y. & Musser, J. M. ( 1996; ). Population genetic analysis of Helicobacter pylori by multilocus enzyme electrophoresis: extensive allelic diversity and recombinational population structure. J Bacteriol 178, 3934–3938.
    [Google Scholar]
  13. Gomes, L. I., Rocha, G. A., Rocha, A. M., Soares, T. F., Oliveira, C. A., Bittencourt, P. F. & Queiroz, D. M. ( 2008; ). Lack of association between Helicobacter pylori infection with dupA-positive strains and gastroduodenal diseases in Brazilian patients. Int J Med Microbiol 298, 223–230.[CrossRef]
    [Google Scholar]
  14. Höcker, M. & Hohenberger, P. ( 2003; ). Helicobacter pylori virulence factors – one part of a big picture. Lancet 362, 1231–1233.[CrossRef]
    [Google Scholar]
  15. Israel, D. A., Salama, N. R., Krishna, U., Rieger, M., Atherton, J. C., Falkow, S. & Peek, R. M., Jr ( 2001; ). Helicobacter pylori genetic diversity within the gastric niche of a single human host. Proc Natl Acad Sci U S A 98, 14625–14630.[CrossRef]
    [Google Scholar]
  16. Kim, Y. S., Kim, N., Kim, J. M., Kim, M. S., Park, J. H., Lee, M. K., Lee, D. H., Kim, J. S., Jung, H. C. & Song, I. S. ( 2009; ). Helicobacter pylori genotyping findings from multiple cultured isolates and mucosal biopsy specimens: strain diversities of Helicobacter pylori isolates in individual hosts. Eur J Gastroenterol Hepatol 21, 522–528.[CrossRef]
    [Google Scholar]
  17. Kuipers, E. J., Israel, D. A., Kusters, J. G., Gerrits, M. M., Weel, J., van der Ende, A., van der Hulst, R. W., Wirth, H. P., Hook-Nikanne, J. & other authors ( 2000; ). Quasispecies development of Helicobacter pylori observed in paired isolates obtained years apart from the same host. J Infect Dis 181, 273–282.[CrossRef]
    [Google Scholar]
  18. Leanza, A. G., Matteo, M. J., Crespo, O., Antelo, P., Olmos, J. & Catalano, M. ( 2004; ). Genetic characterisation of Helicobacter pylori isolates from an Argentinean adult population based on cag pathogenicity island right-end motifs, lspA-glmM polymorphism and iceA and vacA genotypes. Clin Microbiol Infect 10, 811–819.[CrossRef]
    [Google Scholar]
  19. Lu, H., Graham, P. I., Yang, D. & Yamaoka, Y. ( 2005a; ). Duodenal ulcer promoting gene of Helicobacter pylori. Gastroenterology 128, 833–848.[CrossRef]
    [Google Scholar]
  20. Lu, H., Yamaoka, Y. & Graham, D. Y. ( 2005b; ). Helicobacter pylori virulence factors: facts and fantasies. Curr Opin Gastroenterol 21, 653–659.[CrossRef]
    [Google Scholar]
  21. Marshall, D. G., Dundon, W. S., Beesley, S. M. & Smyth, C. J. ( 1998; ). Helicobacter pylori – a conundrum of genetic diversity. Microbiology 144, 2925–2939.[CrossRef]
    [Google Scholar]
  22. Mattar, R., dos Santos, A. F., Eisig, J. N., Rodrigues, T. N., Silva, F. M., Lupinacci, R. M., Iriya, K. & Carrilho, F. J. ( 2005; ). No correlation of babA2 with vacA and cagA genotypes of Helicobacter pylori and grading of gastritis from peptic ulcer disease patients in Brazil. Helicobacter 10, 601–608.[CrossRef]
    [Google Scholar]
  23. Matteo, M. J., Granados, G., Pérez, C. V., Olmos, M., Sanchez, C. & Catalano, M. ( 2007; ). Helicobacter pylori cag pathogenicity island genotype diversity within the gastric niche of a single host. J Med Microbiol 56, 664–669.[CrossRef]
    [Google Scholar]
  24. Prouzet-Mauléon, V., Hussain, M. A., Lamouliatte, H., Kauser, F., Megraud, F. & Ahmed, N. ( 2005; ). Pathogen evolution in vivo: genome dynamics of two isolates obtained 9 years apart from a duodenal ulcer patient infected with a single Helicobacter pylori strain. J Clin Microbiol 43, 4237–4241.[CrossRef]
    [Google Scholar]
  25. Salama, N. R., Gonzalez-Valencia, G., Deatherage, G. B., Aviles-Jimenez, F., Atherton, J. C., Graham, D. Y. & Torres, J. ( 2007; ). Genetic analysis of Helicobacter pylori strain populations colonizing the stomach at different times postinfection. J Bacteriol 189, 3834–3845.[CrossRef]
    [Google Scholar]
  26. Yamaoka, Y. ( 2008; ). Roles of the plasticity regions of Helicobacter pylori in gastroduodenal pathogenesis. J Med Microbiol 57, 545–553.[CrossRef]
    [Google Scholar]
  27. Yamaoka, Y., Kwon, D. H. & Graham, D. Y. ( 2000; ). A M r 34,000 proinflammatory outer membrane protein (oipA) of Helicobacter pylori. Proc Natl Acad Sci U S A 97, 7533–7538.[CrossRef]
    [Google Scholar]
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