1887

Abstract

Phylogenetic analyses of 56 type species of were conducted using the partial nucleotide sequences of the gyrase B–encoding gene (). The interspecies similarities of the gene for the 56 type species were 82.4–99.9 %, which corresponded to 270–2 nt differences in the partial gene sequences of approximately 1200 nt. In comparison with phylogenetic relationships, gene sequence information was generally consistent with that of 16S rRNA gene sequences with minor exceptions. However, the degree of divergence of the gene sequences was approximately 3.6 times greater than those of the 16S rRNA gene, suggesting a higher discriminative power of sequence information compared with 16S rRNA gene sequences for species. The type species were clustered based on sequence similarity values of 93.5 % and above. Among the 56 type species, 38 were distributed in 13 clusters, each comprising 2 to 7 species. The remaining 18 species were classified into an independent cluster, in which the similarity between each species and the other 55 species was less than 93.5 %. Among the eight mycolic acid-containing actinomycete genera in the suborder , was clearly differentiated from the other genera, such as , by gene analyses (similarity values of sequences for and were 75–85 %), indicating that the gene is a useful alternative to the 16S rRNA gene for the determination of phylogenetic relationships between the genus and the seven other actinomycete genera.

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2010-02-01
2019-11-22
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