1887

Abstract

Protozoan parasites of the genus are the causative agents of life-threatening visceral as well as cutaneous and mucocutaneous leishmaniasis. First-line drugs are antimonials, but toxicity and resistance in some endemic areas cause serious problems. In the current study, the antileishmanial activity of the weak oxidant -chlorotaurine (NCT) was investigated. NCT is a derivative of the amino acid taurine produced by granulocytes and monocytes during oxidative burst, but can also be synthesized chemically and used topically as an antiseptic at a concentration of 1 % (55 mM) . NCT susceptibility tests were performed with promastigotes and amastigotes of and . As NHCl is known to increase the activity of NCT by the formation of monochloramine (NHCl), co-treatment assays were included in the study. Mean EC values after 1 h of treatment were 5.94 mM for and 9.8 mM for promastigotes. Co-treatment with 5.5 mM NCT plus 19 mM NHCl led to complete killing of promastigotes of both strains within 15 min. Amastigotes were inactivated by treatment with 2 mM NCT alone. The results of this study indicate a high potential of NCT against species.

Keyword(s): NCT, N-chlorotaurine
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2009-10-01
2019-12-06
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References

  1. Arnitz, R., Sarg, B., Ott, H. W., Neher, A., Lindner, H. & Nagl, M. ( 2006; ). Protein sites of attack of N-chlorotaurine in Escherichia coli. Proteomics 6, 865–869.[CrossRef]
    [Google Scholar]
  2. Ashutosh, Sundar, S. & Goyal, N. ( 2007; ). Molecular mechanisms of antimony resistance in Leishmania. J Med Microbiol 56, 143–153.[CrossRef]
    [Google Scholar]
  3. Beach, D. H., Holz, G. G., Jr & Anekwe, G. E. ( 1979; ). Lipids of Leishmania promastigotes. J Parasitol 65, 201–216.
    [Google Scholar]
  4. Collin, S., Davidson, R., Ritmeijer, K., Keus, K., Melaku, Y., Kipngetich, S. & Davies, C. ( 2004; ). Conflict and kala-azar: determinants of adverse outcomes of kala-azar among patients in southern Sudan. Clin Infect Dis 38, 612–619.[CrossRef]
    [Google Scholar]
  5. Croft, S. L., Sundar, S. & Fairlamb, A. H. ( 2006; ). Drug resistance in leishmaniasis. Clin Microbiol Rev 19, 111–126.[CrossRef]
    [Google Scholar]
  6. Eibl, H. ( 2000; ). Tödliche Tropenkrankheit jetzt heilbar. http://www.mpibpc.gwdg.de/abteilungen/293/PR/00_01/leish.html (accessed 12 February 2009).
  7. Escobar, P., Matu, S., Marques, C. & Croft, S. L. ( 2002; ). Sensitivities of Leishmania species to hexadecylphosphocholine (miltefosine), ET-18-OCH3 (edelfosine) and amphotericin B. Acta Trop 81, 151–157.[CrossRef]
    [Google Scholar]
  8. Fürnkranz, U., Nagl, M., Gottardi, W., Köhsler, M., Aspöck, H. & Walochnik, J. ( 2008; ). Cytotoxic activity of N-chlorotaurine on Acanthamoeba spp. Antimicrob Agents Chemother 52, 470–476.[CrossRef]
    [Google Scholar]
  9. Gebre-Hiwot, A., Tadesse, G., Croft, S. L. & Frommel, D. ( 1992; ). An in vitro model for screening antileishmanial drugs: the human leukaemia monocyte cell line, THP-1. Acta Trop 51, 237–245.[CrossRef]
    [Google Scholar]
  10. Gottardi, W. & Nagl, M. ( 2002; ). Chemical properties of N-chlorotaurine sodium, a key compound in the human defence system. Arch Pharm (Weinheim) 335, 411–421.[CrossRef]
    [Google Scholar]
  11. Gottardi, W. & Nagl, M. ( 2005; ). Chlorine covers on living bacteria: the initial step in antimicrobial action of active chlorine compounds. J Antimicrob Chemother 55, 475–482.[CrossRef]
    [Google Scholar]
  12. Gottardi, W., Hagleitner, M. & Nagl, M. ( 2001; ). The influence of plasma on the disinfecting activity of the new antimicrobial agent N-chlorotaurine-sodium in comparison with chloramine T. J Pharm Pharmacol 53, 689–697.[CrossRef]
    [Google Scholar]
  13. Gottardi, W., Arnitz, R. & Nagl, M. ( 2007; ). N-Chlorotaurine and ammonium chloride: an antiseptic preparation with strong bactericidal activity. Int J Pharm 335, 32–40.[CrossRef]
    [Google Scholar]
  14. Grimm, F., Brun, R. & Jenni, L. ( 1991; ). Promastigote infectivity in Leishmania infantum. Parasitol Res 77, 185–191.[CrossRef]
    [Google Scholar]
  15. Hofer, E., Neher, A., Gunkel, A. R. & Nagl, M. ( 2003; ). In vitro study on the influence of N-chlorotaurine on the ciliary beat frequency of nasal mucosa. Am J Rhinol 17, 149–152.
    [Google Scholar]
  16. Hofman, V., Marty, P., Perrin, C., Saint-Paul, M. C., Le Fichoux, Y., Michiels, J. F., Glaichenhaus, N., Pratlong, F. & Hofman, P. ( 2000; ). The histological spectrum of visceral leishmaniasis caused by Leishmania infantum MON-1 in acquired immune deficiency syndrome. Hum Pathol 31, 75–84.[CrossRef]
    [Google Scholar]
  17. Kontny, E., Grabowska, A., Kowalczewski, J., Kurowska, M., Janicka, I., Marcinkiewicz, J. & Maśliński, W. ( 1999; ). Taurine chloramine inhibition of cell proliferation and cytokine production by rheumatoid arthritis fibroblast-like synoviocytes. Arthritis Rheum 42, 2552–2560.[CrossRef]
    [Google Scholar]
  18. Marcinkiewicz, J., Grabowska, A., Bereta, J. & Stelmaszynska, T. ( 1995; ). Taurine chloramine, a product of activated neutrophils, inhibits in vitro the generation of nitric oxide and other macrophage inflammatory mediators. J Leukoc Biol 58, 667–674.
    [Google Scholar]
  19. Mathur, P., Samantaray, J. C., Vajpayee, M. & Samanta, P. ( 2006; ). Visceral leishmaniasis/human immunodeficiency virus co-infection in India: the focus of two epidemics. J Med Microbiol 55, 919–922.[CrossRef]
    [Google Scholar]
  20. Nagl, M. & Gottardi, W. ( 1996; ). Enhancement of the bactericidal efficacy of N-chlorotaurine by inflammation samples and selected N-H compounds. Hyg Med 21, 597–605.
    [Google Scholar]
  21. Nagl, M., Larcher, C. & Gottardi, W. ( 1998a; ). Activity of N-chlorotaurine against herpes simplex- and adenoviruses. Antiviral Res 38, 25–30.[CrossRef]
    [Google Scholar]
  22. Nagl, M., Miller, B., Daxecker, F., Ulmer, H. & Gottardi, W. ( 1998b; ). Tolerance of N-chlorotaurine, an endogenous antimicrobial agent, in the rabbit and human eye – a phase I clinical study. J Ocul Pharmacol Ther 14, 283–290.[CrossRef]
    [Google Scholar]
  23. Nagl, M., Pfausler, B., Schmutzhard, E., Fille, M. & Gottardi, W. ( 1998c; ). Tolerance and bactericidal action of N-chlorotaurine in a urinary tract infection by an omniresistant Pseudomonas aeruginosa. Zentralbl Bakteriol 288, 217–223.[CrossRef]
    [Google Scholar]
  24. Nagl, M., Hess, M. W., Pfaller, K., Hengster, P. & Gottardi, W. ( 2000; ). Bactericidal activity of micromolar N-chlorotaurine: evidence for its antimicrobial function in the human defense system. Antimicrob Agents Chemother 44, 2507–2513.[CrossRef]
    [Google Scholar]
  25. Nagl, M., Lass-Floerl, C., Neher, A., Gunkel, A. R. & Gottardi, W. ( 2001; ). Enhanced fungicidal activity of N-chlorotaurine in nasal secretion. J Antimicrob Chemother 47, 871–874.[CrossRef]
    [Google Scholar]
  26. Nagl, M., Nguyen, V. A., Gottardi, W., Ulmer, H. & Höpfl, R. ( 2003; ). Tolerability and efficacy of N-chlorotaurine in comparison with chloramine T for the treatment of chronic leg ulcers with a purulent coating: a randomized phase II study. Br J Dermatol 149, 590–597.[CrossRef]
    [Google Scholar]
  27. Park, E., Schuller-Levis, G. & Quinn, M. R. ( 1995; ). Taurine chloramine inhibits production of nitric oxide and TNF-α in activated RAW 264.7 cells by mechanisms that involve transcriptional and translational events. J Immunol 154, 4778–4784.
    [Google Scholar]
  28. Reeves, E. P., Nagl, M., O'Keeffe, J., Kelly, J. & Kavanagh, K. ( 2006; ). Effect of N-chlorotaurine on Aspergillus, with particular reference to destruction of secreted gliotoxin. J Med Microbiol 55, 913–918.[CrossRef]
    [Google Scholar]
  29. Singh, S. P., Reddy, D. C., Rai, M. & Sundar, S. ( 2006; ). Serious underreporting of visceral leishmaniasis through passive case reporting in Bihar, India. Trop Med Int Health 11, 899–905.[CrossRef]
    [Google Scholar]
  30. Sundar, S. & Rai, M. ( 2005; ). Treatment of visceral leishmaniasis. Expert Opin Pharmacother 6, 2821–2829.[CrossRef]
    [Google Scholar]
  31. Teuchner, B., Nagl, M., Schidlbauer, A., Ishiko, H., Dragosits, E., Ulmer, H., Aoki, K., Ohno, S., Mizuki, N. & other authors ( 2005; ). Tolerability and efficacy of N-chlorotaurine in epidemic keratoconjunctivitis – a double-blind, randomized, phase-2 clinical trial. J Ocul Pharmacol Ther 21, 157–165.[CrossRef]
    [Google Scholar]
  32. Yardley, V., Croft, S. L., De Doncker, S., Dujardin, J. C., Koirala, S., Rijal, S., Miranda, C., Llanos-Cuentas, A. & Chappuis, F. ( 2005; ). The sensitivity of clinical isolates of Leishmania from Peru and Nepal to miltefosine. Am J Trop Med Hyg 73, 272–275.
    [Google Scholar]
  33. Yazdanbakhsh, M., Eckmann, C. M. & Roos, D. ( 1987; ). Killing of schistosomula by taurine chloramine and taurine bromamine. Am J Trop Med Hyg 37, 106–110.
    [Google Scholar]
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